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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cryo-EM data for the wt PV2-CP17 complex were collected at 300 kV on a Titan Krios (Thermo Fisher Scientific) equipped with a K3 DED and GIF Quantum energy filter (Gatan ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were plated at 8×105 per well in six-well plates and transfected with gRNA complex (crRNA:tracrRNA 10nM) in antibiotic-free medium using Lipofectamine RNA iMAX Reagent (Invitrogen #13778-150), according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... The transfection is performed by adding the pre-mixed complex of siRNA with the reagent in reduced serum media (Opti-MEM; Invitrogen, #31985062) to cells without a media change ...
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bioRxiv - Genomics 2024Quote: ... were delivered in the form of ribonucleoprotein (RNP) complex via Neon Electric Transfection System (10 µl kit; catalogue no.: MPK1096; Thermo Fisher). First ...
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bioRxiv - Cancer Biology 2024Quote: ... washed in PBS and 5 x 105 cells were electroporated in the presence of the crRNA/ tracrRNA.Cas9 complex using the NeonTM Electroporation system (Thermofisher scientific, MPK5000) (4 pulses of 20ms at 1400V) ...
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bioRxiv - Biochemistry 2024Quote: INIP and the TRR complex were labeled on N-termini using AF488 dye (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Before labeling ...
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bioRxiv - Molecular Biology 2024Quote: The 1A/3xFLAG-MYCN complex was desalted into 200 mM ammonium acetate using 75 μl Zeba spin desalting columns (ThermoFisher Scientific). Samples were analysed by nanoelectrospray ionisation MS using a quadrupole-orbitrap MS (Q-Exactive UHMR ...
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bioRxiv - Synthetic Biology 2024Quote: Huh7 cells with p62/SQSTM1 CRISPR–Cas9 knockout were generated through electroporation of Cas9– sgRNA ribonucleoprotein complex using Neon™ Transfection System (Invitrogen). Two Alt-R™ CRISPR-Cas9 crRNA (IDT ...
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bioRxiv - Bioengineering 2024Quote: ... The absorbance of the resulting purple complex was measured at 570 nm using a Multiskan™ microplate reader (Thermo Scientific™). Protein concentrations were determined by comparison to the BSA standard curve.
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bioRxiv - Cancer Biology 2024Quote: ... Hs578T and BT-549 cells were co-transfected with a ribonucleoprotein (RNP) complex comprising Cas9 and guide RNA (gRNA) according to manufacturer’s instructions (Invitrogen™, #CMAX00015). The crRNA and tracrRNA (Integrated DNA Technologies ...
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Cancer Biology 2021Quote: ... or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies). After 4 washes in IP buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-bound proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Waltham, MA, # 34096) and imaged using a FluoroChem M (Protein Simple ...
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bioRxiv - Biochemistry 2021Quote: ... for spike protein or RBD detection and mouse anti-beta tubulin antibody (1:5000; Thermo Fisher; MA5-16308) as a loading control ...
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bioRxiv - Molecular Biology 2021Quote: ... and the proteins analyzed by Western blotting using 1:2,000 goat anti-gp120 polyclonal antibody (Thermo Fisher Scientific) and 1:2,000 HRP-conjugated rabbit anti-goat IgG (Thermo Fisher Scientific ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... The leftover samples were precipitated with anti-FLAG antibody which was prebound to Protein G Dynabeads (Thermo Fisher). The 50 µL of saved chromatin and immunoprecipitated DNA were de-crosslinked in a 65°C water bath overnight ...
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bioRxiv - Plant Biology 2020Quote: ... GSTU7-V5 fusion protein was detected with anti-V5 mouse monoclonal antibody (1:5,000 dilution, from Invitrogen #R96025) and a peroxidase-conjugated anti-mouse secondary antibody (1:10,000 dilution from KPL ...
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bioRxiv - Microbiology 2020Quote: ... PhoU2-HA proteins were detected with an anti-HA rabbit polyclonal antibody (Invitrogen, 71-5500, 1:500 dilution) as primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... The antibody-bound proteins were detected by incubating with Pierce ECL substrate or SuperSignal West Femto substrate (ThermoFisher) and visualize on a AmershamTM Imager 600 system (GE Healthcare) ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were conjugated to 30 uL of Protein G Magnetic Beads (Dynabeads, Thermo Fisher Scientific 10-003-D), per ChIP ...
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bioRxiv - Cell Biology 2021Quote: ... For detection of ubiquitylated proteins, slides were first blocked with antibody blocking buffer (PBS, 0.1% Synthetic Block (Invitrogen)) for 10 min followed by 1.5 hours incubation at 4°C with anti-ubiquitin chain antibody FK2 (1 µg/ml FK2 in antibody blocking buffer) ...
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bioRxiv - Biochemistry 2022Quote: ... With the following exceptions: the nitrocellulose-coated coverslips were incubated with protein or with anti-GST antibody (Invitrogen) for 4 min ...
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bioRxiv - Microbiology 2022Quote: ... Western blots were performed on the immunoprecipitated protein samples using the mouse-anti-HA tag (2-2.2.14) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Western blots were performed on the immunoprecipitated protein samples using the mouse-anti-eGFP (F56-6A.1.2.3) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Fifty μL of 1:3000 diluted mouse anti-SARS-CoV-2 Spike Protein S1 monoclonal antibody (GT623, Invitrogen) in 1% BSA-PBS blocking buffer was added and incubated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2022Quote: AKAP12 was immunoprecipitated from HSCs or hepatocytes using an AKAP12 antibody-conjugated protein A/G column (Thermo Scientific). The AKAP12 beads were submitted to Applied Biomics ...
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bioRxiv - Microbiology 2021Quote: ... Virion lysates were analyzed by SDS-PAGE and western blot using Spike Protein S2 Monoclonal Antibody (1A9) (Invitrogen) (1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with HA antibody (at a conc. of 1:100) pre-bound Protein-G Agarose bead (Invitrogen) or with pre-blocked anti-FLAG M2 beads and rotated overnight at 4□C ...
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bioRxiv - Microbiology 2021Quote: ... Antibody supernatants were passed three times over columns packed with immobilized Protein G resin (Thermo Fisher, catalog #20397) as previously described (Scherer et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Cbf11-TAP protein was verified by western blot with an anti-TAP antibody (Thermo Scientific, CAB1001). Prototrophic cbf11-TAP strain was then prepared by standard crossing and revalidated.
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bioRxiv - Genetics 2022Quote: ... Rad51 protein was detected by incubating the membranes with rabbit polyclonal antibody anti-Rad51 (1:2000, Thermo Fisher Scientific Cat# PA5-34905 ...
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bioRxiv - Plant Biology 2022Quote: ... The antibody used for immunoprecipitation was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) for 3 h with gentle rotation at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (RBD, 1:1,000, Invitrogen cat. no. PA5-114451). All primary antibodies were diluted in PBST + 5% milk and applied to membranes for ≥16 hours at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... 40 µg of Oct4 antibody (R&D, AF1759) was coupled to Dynabeads protein G (Thermo Fisher Scientific, 10009D) for 2 hours at room temperature for each sample ...
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bioRxiv - Genomics 2021Quote: Argonaute IPs were carried out using affinity purified rabbit antibodies pre-bound to Protein A Dynabeads (Fisher Scientific) (5-10 ug of antibody per 100 ul of beads ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... Final antibody concentrations were determined by measuring the A562 using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at −80°C prior to use.
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bioRxiv - Neuroscience 2022Quote: Solubilisates were directly incubated with 7.5 µg Kv4.3 antibodies (Alomone, #APC-017) immobilized on Protein A Dynabeads (Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-chromatin complexes were pulled-down using Protein A/G magnetic beads (Dy-10001D and Dy-10003D, Invitrogen), washed and then eluted ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Neuroscience 2022Quote: ... 50 μg/ml of GPC3core protein was mixed with Alexa568-conjugated anti-hIgG antibody (Thermo Fisher Scientific, A21090) in PBS ...
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bioRxiv - Plant Biology 2024Quote: ... The EIN2-C native antibody was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) with 1% BSA for 3 h with gentle rotation at 4 °C and incubated at 4 °C after cleared cell fractionation addition with gentle rocking overnight for Co-IP ...
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bioRxiv - Neuroscience 2024Quote: ... The presence of the Cas9 protein was tested using antibody against the V5 tag (ref R960-25, Invitrogen). Secondary antibodies used for Western blot were labelled with HRP (Jackson Immuno Research) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by horseradish peroxidase-conjugated secondary antibody using SuperSignal West Pico PLUS chemiluminescent substrate (ThermoFisher Scientific) and imaged with an Invitrogen iBright FL1500 imaging system (ThermoFisher Scientific) ...