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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used for protein visualization were the following: rabbit AF488 (1:1000, Invitrogen), guinea pig Cy3 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... NAb™ Protein L Spin Kit for all Kappa chain antibodies (all from Thermo Scientific). Purified intact antibodies (40 μg IgG ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-23 specific antibody-crosslinked to protein A/G Magnetic-agarose (ThermoFisher Cat. No. 78609) was used ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies against the following proteins were used for Blue Native Page: NDUFB8 (CI, Thermofisher 459210), SDHB (CII ...
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bioRxiv - Developmental Biology 2024Quote: ... antibody:bead complexes of 5ul (1µg/uL) antibody and 25ul (30mg/mL) Protein G DynabeadsTM (Invitrogen) were prepared ...
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bioRxiv - Cell Biology 2024Quote: ... the formed antibody-immunocomplexes were incubated with 100 µL Protein G magnetic DynabeadsTM (Invitrogen, 10004D) for 1 h at room temperature with head-to-tail rotation allowing the complexes to bind the magnetic beads ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μg per antibody per sample) using recombinant biotinylated Protein L (Thermo Fisher Scientific, #29997) for 16–18 h on a rotator (4 °C ...
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bioRxiv - Microbiology 2024Quote: ... anti-CEA rabbit polyclonal antibodies (supplemental table 2) were immobilized onto protein G Dynabeads (Invitrogen) and incubated with culture supernatant at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatants were incubated with primary antibodies and Protein A/G magnetic beads (Thermo Scientific, 88803) overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were purified from the conditioned media using Nab Protein G spin columns (ThermoFisher, 89957) and eluted in Pierce IgG Elution Buffer (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... SNAP-23 specific antibody-crosslinked to protein A/G Magnetic-agarose (ThermoFisher Cat. No. 78609) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the antibody-stained protein signal was amplified and visualized using SuperSignal (ThermoFisher 34577). Blots were imaged with a ChemiDoc MP Imaging system (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-HA or normal mouse IgG antibody was incubated with DynabeadTM Protein G beads (Invitrogen) for 1 hr at 4°C to form the Dynabead-bound antibody complexes ...
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bioRxiv - Genetics 2024Quote: ... and immunoprecipitated with 10 µL of HA tag antibody bounded to Protein A Dynabeads (Invitrogen). Quantitative PCR were performed at given regions using SYBR Green PCR Master Mix (Biorad ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Cell Biology 2022Quote: ... the DNA-lipid complex (1.25 μg DNA: 3.75 μL Lipofectamine 3000 in 250 μL Opti-MEM; Thermo Fisher) was added to 1.62×105 cells as they were seeding onto a 35 mm dish with 2 mL of growth medium ...
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bioRxiv - Biochemistry 2020Quote: ... The complex formed with Pi was measured at 630 nm in a Multiskan GO microplate reader (Thermo Scientific).
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bioRxiv - Biophysics 2021Quote: Cryo-EM data for the T-plastin–actin (–Ca2+) complex were recorded on a Titan Krios (ThermoFisher / FEI) at the Rockefeller University operated at 300 kV equipped with a Gatan K2 Summit camera ...
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bioRxiv - Biochemistry 2021Quote: ... The gel band corresponding to the cross-linked complex was excised and digested with trypsin (Thermo Scientific Pierce) [107] and the resulting tryptic peptides were extracted and desalted using C18 StageTips [108] ...
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bioRxiv - Biochemistry 2020Quote: ... Data for the pre-GAP complex structure was acquired on a Titan Krios electron microscope (Thermo Fisher Scientific) operated at 300 kV ...
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bioRxiv - Biochemistry 2020Quote: ... Data for the post-GAP complex structure was acquired on a Talos Arctica electron microscope (Thermo Fisher Scientific) operated at 200 kV ...
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bioRxiv - Cell Biology 2020Quote: ... cells were immunolabeled on ice for 10 min with Alexa Fluor 647 EGF complex (Molecular Probes, Invitrogen, E35351) in PBS containing 2% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... cells were immunolabeled on ice for 10 min with Alexa Fluor 647 EGF complex (Molecular Probes, Invitrogen, E35351) in PBS containing 2% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... diluted at 1:300 followed by 1hr incubation with avidin-biotin complex (1:1:100; cat# 32020; ThermoFisher) and 1hr incubation with TSA plus Cyanine-3 (1:300 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 times) and immediately incubated for 30 min with streptavidin–peroxidase complex diluted 1:400 in TBS (Invitrogen). The chromogen was 3-30-diaminobenzidine (Vector Laboratories) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the RNP complex was then delivered into 293T cells using Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Invitrogen, CAT# CMAX00008) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Movies of the Ab1485-BG505-8ANC195 complex were collected on a Talos Arctica transmission electron microscope (Thermo Fisher) operating at 200 kV using SerialEM automated data collection software (82 ...
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bioRxiv - Biochemistry 2022Quote: Cryo-EM data set of the FERRY complex was collected on a Titan Krios TEM (Thermo Fisher Scientific), equipped with an Cs-Corrector and in-column energy filter ...
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bioRxiv - Immunology 2023Quote: ... and 40 µL per mL of complex of 1 M formaldehyde prepared from 37 % stock (Thermo Fisher, 10630813). The complex was incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The phosphopeptide enrichment was performed with High-Select TiO2 Phosphopeptide Enrichment for complex samples -kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... The S-GSAS/6P:H18 complex cryo-grid was imaged in a Talos Arctica electron microscope (Thermo Fisher Scientific) operating at 200 kV ...
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bioRxiv - Immunology 2023Quote: ... All constructs were transiently co-transfected into HEK Expi293F cells using ExpiFectamine to produce SIgA complex (Thermo Fisher). Six days after transfection ...
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bioRxiv - Biochemistry 2024Quote: The GPR65-Gs pH 6 complex was concentrated to 11 mg/mL supplemented with 0.05% CHAPS (Thermo Fisher) and 3 µL was applied onto a glow-discharged 300 mesh 1.2/1.3 gold grid covered in a holey gold film (UltrAufoil) ...
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bioRxiv - Biochemistry 2024Quote: The GPR4-Gs pH 6 complex was concentrated to 14 mg/mL supplemented with 0.05% CHAPS (Thermo Fisher) and 3 µL was applied onto a glow-discharged 300 mesh 1.2/1.3 gold grid covered in a holey gold film (UltrAufoil) ...
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bioRxiv - Biophysics 2024Quote: ... Cryo-EM data collection for the COP-1 small complex was performed on a Titan Krios G3i (ThermoFisher) equipped with a Gatan K3 direct electron detector and BioQuantum GIF at the University of Chicago Advanced Electron Microscopy Core Facility (RRID:SCR_019198) ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands were detected using a primary antibody followed by an HRP-conjugated secondary antibody (rabbit, Life technologies #G21234, or mouse, Invitrogen #G21040) and visualized via chemiluminescence using Clarity Western ECL Substrate and the ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Genomics 2021Quote: ... Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody, cat no. PA5-17511, Thermo Fisher Scientific, Rockford, USA) and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 6μg of indicated antibodies or control IgG antibodies were incubated with 70 µL Dynabeads Protein G beads (Thermo Fisher Scientific, Cat#10004D) in IP buffer (2mM EDTA ...
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bioRxiv - Molecular Biology 2021Quote: ... The bTMP-DNA complex in TE was immunoprecipitated using avidin conjugated to magnetic beads (Dynabeads MyOne Streptavidin Invitrogen, 65001) for 2 h at room temperature and then overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... with 20 min lipofectamine/mimic complex formation in Opti-MEM® I Reduced Serum Medium (31985-062, Life Technologies) and cells incubated in DMEM culture as described above with no penicillin or streptomycin added ...
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bioRxiv - Cell Biology 2021Quote: ... The purified FLAG-Atg1 complex was resolved by SDS-PAGE and analyzed by Sypro Ruby staining (Thermo Fisher, S12000).
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bioRxiv - Biophysics 2022Quote: The Cas7-11 ternary complex micrographs were collected using EPU software on the Krios G3i cryo TEM (ThermoFisher Scientific) equipped with Gatan Bioquantum K3 direct electron detector (Gatan ...
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bioRxiv - Genomics 2022Quote: ... The RNP complex was transfected into cells by electroporation with Neon transfection 10 µl kit (Thermo Fisher Scientific, MPK1096). To measure the efficiency of genome editing in each guide RNA ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa or U2OS cells were electroporated with the sgRNA/Cas9 complex using the Neon transfection system (Thermo Fisher Scientific) at 1005 V ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:25∼28 molar ratio for 2 hrs on ice ...
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bioRxiv - Cell Biology 2022Quote: Cryo-EM images of the TFEB-Rag-Ragulator complex were recorded under a Talos Arctica microscope (Thermo Fisher Scientific) operated at 200 kV ...
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bioRxiv - Genomics 2022Quote: ... For each experiment LNCaP-dCas9-KRAB cells were transfected with 30 nM sgRNA complex using Lipofectamine RNAiMAX (Invitrogen, 13778030). 48 hours post-transfection cells were treated with media containing 10 µM enzalutamide or DMSO for a further 24 hours.
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bioRxiv - Neuroscience 2022Quote: ... BODIPY FL C5-Lactosylceramide complex to BSA (Cat# B34402) and BODIPY FL ganglioside(Cat# B13950) were obtained from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 110µl TRAK1-KIF5B complex eluted from the beads were incubated with 1.2mM bis(sulfosuccinimidyl)suberate (BS3) (Thermo Fisher Scientific) for 30min at 4°C ...