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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The EIN2-C native antibody was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) with 1% BSA for 3 h with gentle rotation at 4 °C and incubated at 4 °C after cleared cell fractionation addition with gentle rocking overnight for Co-IP ...
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bioRxiv - Microbiology 2024Quote: ... or 2 μg of Flag Tag antibody (mouse) conjugated with Dynabeads Protein G Magnetic Beads (Invitrogen, 10004D, USA), or anti-HA magnetic Beads (Abmart ...
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bioRxiv - Cancer Biology 2024Quote: ... or with Protein A agarose beads incubated with 2 μg anti-LONP1 antibody (ThermoFisher Scientific 15440-1-AP) overnight (16 hours ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoprecipitation was done using 1 µg of anti-GFP antibody (MBL) bound to Dynabeads protein A (Thermo Fisher). After washing and elution ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by horseradish peroxidase-conjugated secondary antibody using SuperSignal West Pico PLUS chemiluminescent substrate (ThermoFisher Scientific) and imaged with an Invitrogen iBright FL1500 imaging system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... immunoprecipitation of Hfq-RNA complexes with an anti-Flag primary antibody using DynabeadsTM Protein A (Thermo Fisher, 10002D) overnight at 4 ℃ ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... Final antibody concentrations were determined by measuring the A562 using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at −80°C prior to use.
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bioRxiv - Neuroscience 2022Quote: Solubilisates were directly incubated with 7.5 µg Kv4.3 antibodies (Alomone, #APC-017) immobilized on Protein A Dynabeads (Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-chromatin complexes were pulled-down using Protein A/G magnetic beads (Dy-10001D and Dy-10003D, Invitrogen), washed and then eluted ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Plant Biology 2023Quote: ... Beads that were made specific for DEAP2 by attaching the antibody to Protein G-coupled beads (Dynabeads, ThermoFisher) according to protocol ...
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bioRxiv - Microbiology 2024Quote: ... cell lysates were incubated with protein A beads (Cytiva Life Sciences) and anti-myc antibodies (132500, ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... the antibodies were conjugated with the beads by first washing 25ul of Protein A Dynabeads (Invitrogen, Cat.N: 10002D) with cold 0.5% BSA in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were detected by SDS-PAGE and Western immunoblotting using anti-His antibodies (Invitrogen Canada Inc., Burlington, ON). Fractions were monitored for alkaline phosphatase activity by assay with 2 mM p-nitrophenyl phosphate ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies were purified from the collected sera using the NAb Protein A Plus Spin Kit (Thermo Scientific 89948) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... IPs were incubated with 6µg antibody overnight at 4°C and purified using Protein A magnetic beads (Invitrogen). After washing and eluting purified IP ...
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bioRxiv - Cell Biology 2024Quote: ... VIT-2::GFP proteins were visualized by western blotting using an anti-GFP antibody (A-11122, Thermo Fisher) at a dilution of 1:2500.
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Microbiology 2021Quote: 293T cells at 30% density in each well of 96 well plates were reverse transfected with 0.60 µg of plasmid in complex with 0.15 µL of lipofectamine 2000 (Life Technologies, Cat. No. 11668019). Twenty-four hours after transfection ...
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bioRxiv - Biophysics 2020Quote: ... while hNAA25 contained a Tobacco-etch virus (TEV)-cleavable N-terminal 6xHis-tag. Human NatB complex (hNAA201-163/hNAA25FL) was obtained by coexpressing these two plasmids in Sf9 (S. frugiperda) cells (ThermoFisher, cat# 12659017), and purified as described previously[35] ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNP complex was combined in 20 μl Opti-MEM at a final concentration of 2 μM (ThermoFisher Scientific catalog number 31985070) along with 5 μM of repair template (custom Ultramer ssDNA ...
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bioRxiv - Biochemistry 2022Quote: ... Data for DNMT1:H3Ub2:DNAmCG/fCG ternary complex was collected at the University of Tokyo on a 300 kV Titan Krios electron microscope (Thermo Fisher Scientific) with a K3 direct electron detector (Gatan ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: The knockouts were performed using the Alt-R CRISPR-Cas9 system (IDT) and reverse transfection of a Cas9:guideRNA ribonucleoprotein complex using Lipofactamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: The knockouts were performed using the Alt-R CRISPR-Cas9 system (Integrated DNA Technologies) and reverse transfection of a Cas9:guideRNA ribonucleoprotein complex using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... mixed with 20μM of each RNP complex and electroporated (1600 volts, 10 ms, 3 pulses) using a Neon transfection system (Thermo Fisher, Waltham, MA). Cells were transferred into 90 μl TCM pre- warmed to 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... visualized by incubation with avidin-biotin-peroxidase complex (Ultrasensitive and Immunopure ABC Peroxidase staining kits for the striatum and for the SNpc, respectively; Thermo Fisher Scientific) using the VectorSG Peroxidase Substrate Kit (Vector Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... OCI-AML-2 and OCI-AML-3 cells were electroporated with sgRNA/Cas9 complex using the Neon transfection system (Thermo Fisher Scientific) under the condition of 1350 V ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then washed in TBS and incubated for 1 hour in TBS containing streptavidin-biotin-peroxidase complex (ABC 1:1,000 TBS; ThermoFisher Scientific, Product# 32020), washed again in TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of CAK complex were applied to a plasma cleaned grid (Tergeo plasma cleaner, PIE scientific) mounted in a Vitrobot Mark IV (Thermo Fisher Scientific) operated at 5°C and 100% humidity ...
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bioRxiv - Genetics 2024Quote: ... S1F/TERT-1 and Mel-ST cell lines were electroporated with ribonucleoprotein complex via Neon™ Electroporation System (Thermofisher Scientific, Massachusetts, USA). To maximize the genome-editing efficacy ...
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bioRxiv - Systems Biology 2023Quote: ... at a 3:1 Fugene volume:DNA mass ratio for transfection complex formation in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific #31985070). Puromycin (Sigma Aldrich #P9620 or #P4512-1MLX10 ...
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bioRxiv - Cell Biology 2022Quote: Cryo-EM images of the Raptor-TFEB-Rag-Ragulator complex and mTORC1-TFEB-Rag-Ragulator megacomplex were recorded under a Titan Krios G3 microscope (Thermo Fisher Scientific) equipped with a Gatan Quantum energy filter (slit width 20 eV ...
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bioRxiv - Genomics 2022Quote: DGs were dissected by removing the whole sting complex of a dry-ice anesthetized bee and placing it in a drop of RNAlater (Invitrogen, Carlsbad, CA) to keep the tissue cold and prevent degradation ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells of F10 sub clone of the KBM7-1-55-S2-24 line were transfected with the Cas9/gRNA complex using electroporation by Neon Transfection system (Thermo Fisher Scientific) according to manufacture recommendations ...
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bioRxiv - Immunology 2023Quote: ... The SA-PE*DL650 complex concentration was calculated by measuring the absorbance of PE at 566nm using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). The SA-PE*DL650 complex was then incubated with 10-fold molar excess of biotinylated HLA-A2-M1 for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were seeded at 8 × 104 cells/ml and transfected with the RNP complex using Lipofectamine CRISPRMAX transfection reagent (Catalog # CMAX00008, Thermo Fisher Scientific). 5-6 days post transfection ...
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bioRxiv - Neuroscience 2024Quote: ... containing the indicated amounts of recombinant or brain-derived assemblies in complex with 1 µl of lipofectamine 2000 (Thermo Fisher Scientific, # 11668019). Cells were incubated at 37 °C for 1 h and the lipofectamine-mediated delivery of the assemblies were stopped by the addition of 100 µl DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: The SM-FP complex tissue from the left ankle joint was immediately infiltrated into RNAlater™ Stabilization Solution (Invitrogen, Waltham, MA, USA) and stored at −20 °C (n=24) ...
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bioRxiv - Immunology 2024Quote: ... LN cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with Live/Dead dye (Aqua, Invitrogen, cat# L34966), anti-human IgM (FITC ...
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bioRxiv - Molecular Biology 2024Quote: The cryo-EM data of the hAgo2 R315V/H316A-guide-target RNA complex were collected on a TALOS Arctica transmission electron microscope (Thermo Fisher Scientific) with a field emission gun operating at an accelerating voltage of 200 kV and equipped with a Falcon 4i Direct Electron Detector ...
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Structural Model of Bacteriophage P22 Scaffolding Protein in a Procapsid by Magic-Angle Spinning NMRbioRxiv - Biochemistry 2024Quote: ... The mixture was incubated at 25°C for 1 hour before sedimentation of the complex at 175,000 g for 20 minutes using a RP80AT rotor (Thermo Scientific, Waltham MA), in a Sorvall RC M120EX ultracentrifuge (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: Cryo-EM images of the mTORC1–Rheb–Rag–Ragulator–4EBP1 complex on liposomes were recorded using a Titan Krios G3 microscope (Thermo Fisher Scientific) equipped with a Gatan Quantum energy filter (slit width 20 eV ...
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bioRxiv - Bioengineering 2024Quote: ... and the eye was disinfected in a 10% w/v iodine solution (polyvinylpyrrolidone-iodine complex [Thermo Fisher Scientific, Roskilde, Denmark] in PBS). The anterior segment of the eye was cut off and blunt forceps were used to remove the vitreous ...
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bioRxiv - Genomics 2020Quote: ... and Protein A or Protein G Dynabeads (Invitrogen). After incubation ...
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bioRxiv - Immunology 2022Quote: ... or with Dynabeads protein A/protein G (Thermofisher) combined with Abatacept ...
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bioRxiv - Biochemistry 2021Quote: ... protein marker (NativeMark Unstained protein standard, LC0725, ThermoFisher) was diluted 50x in the working buffer (40 mM HEPES pH 7.5) ...
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bioRxiv - Immunology 2020Quote: ... protein A and protein G magnetic beads (Invitrogen) were added to capture the antibody-chromatin complexes ...
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bioRxiv - Immunology 2021Quote: ... For protein purification protein G dynabeads (Thermo Fisher), coupled with 2.5 µg of antibody (Table S8 ...
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bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours ...