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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Magentic dynabeads Protein G or Protein A (Invitrogen) were washed with 0.05% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein A and Protein G Dynabeads (Thermofisher Scientific) were washed twice with IP dilution buffer (150 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for two hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and Protein A or Protein G Dynabeads (Invitrogen) at 4 ℃ overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was precipitated using Protein G Dynabeads (Invitrogen), with rabbit anti-IgM IgG linker antibodies for the H2Aubi119 ChIP ...
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bioRxiv - Microbiology 2020Quote: ... and proteins detected using specific primary (diluted at 1:1000) and HRP-secondary antibodies (ThermoFisher, diluted at 1:10,000). Protein bands were detected using Pierce SuperSignal West Pico chemiluminescent substrate kit (Pierce ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Molecular Biology 2020Quote: ... Each half of diluted lysate is incubated with 1/10 volume of antibody coated Protein G Dynabeads™ (Invitrogen) for 40 min at 4°C by end to end rotation ...
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bioRxiv - Neuroscience 2021Quote: ... were used to bind to presynaptic axonal regions and anti-post synaptic density protein 95 (PSD-95) antibodies (Invitrogen, Carlsbad ...
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bioRxiv - Cell Biology 2022Quote: ... The endogenous antibodies were added for 4 °C overnight reaction before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Immunology 2020Quote: ... Antibody concentration was determined by measuring the amount of A280 protein using Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA). Conjugated antibodies were then diluted in PBS-based antibody stabilization solution or LowCross-Buffer (Candor Bioscience GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then initially stained with microtubule-associated protein 2 (MAP2) monoclonal primary antibody (M13) (ThermoFisher Scientific,ON, Canada) at a 1:300 dilution then incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Vps54/scat protein was detected using HRP secondary antibodies with the SuperSignal West Pico ECL chemiluminescent substrate (ThermoFisher 34580) and scanned on a C-DiGit blot scanner ...
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bioRxiv - Microbiology 2020Quote: ... hybridoma cells were pelleted at 1000 x g and the supernatant containing antibody P3.2 was filtered with 0.22 μm filter units (Nalgene) before being loaded onto Protein G resin (Thermo Fisher) previously equilibrated with 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... and immunoprecipitation of the precleared lysate was performed using polyclonal anti-core antibody and protein A sepharose beads (Invitrogen). Immunoprecipitated complexes were washed twice in buffer B (1% Triton X100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed with flow buffer 1X and incubated with protein A-488 secondary antibody conjugate (Invitrogen, P11047) for 20 minutes (1:1000 dilution) ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.6) and subjected to preclearing and overnight incubation with Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific). For silver staining of SDS-gels PierceTM Silver Stain for mass spectrometry (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... MX2-Flag proteins were detected using anti-Flag monoclonal M2 and secondary antibody conjugated to Alexa 594 (Invitrogen, A21203). Endogenous NUP358 was detected using rabbit anti-NUP358 antibody (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with 2.5 µg of a SARS-CoV spike protein polyclonal antibody (PA1-41165, Thermo Fisher Scientific) for 30 min on ice before washing with 2.5 mL of FACS buffer and centrifuging at 1750 RPM for 7 min ...
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bioRxiv - Genomics 2022Quote: ... diluted chromatin was incubated for 2 h with antibodies (2.5 μg/100 μL) coupled to magnetic Dynabeads Protein A (Invitrogen). ChIP samples were washed 4 times in ice-cold RIPA buffer ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Microbiology 2021Quote: ... Horse-radish peroxidase-conjugated secondary antibodies were used to visualize proteins using Pierce ECL Western Blot Substrate (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Cy5 or Alexa 647 secondary antibody pre-absorbed against a range of mammalian serum proteins (Jackson Immunoresearch and ThermoFisher). This protocol was modified for FISH as described (Radford and McKim ...
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bioRxiv - Developmental Biology 2020Quote: ... Untagged SMO (Fig. S5C) was captured using SMO antibody covalently conjugated to Protein A Dynabeads (Thermo Fisher Scientific, Invitrogen). Immunoprecipitates were washed once with Ubiquitination Wash Buffer A (Ubiquitination Lysis Buffer B + 0.5% SDS) ...
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bioRxiv - Developmental Biology 2020Quote: ... Untagged SMO (Fig. S5C) was captured using SMO antibody covalently conjugated to Protein A Dynabeads (Thermo Fisher Scientific, Invitrogen). Immunoprecipitates were washed once with Ubiquitination Wash Buffer A (Ubiquitination Lysis Buffer B + 0.5% SDS) ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... mCherry-fused Piezo1 protein was probed with rat monoclonal anti-mCherry antibody (Clone 16D7, ThermoFisher Scientific; 1:1,000 dilution); the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G magnetic beads were incubated with anti-HA antibody before being cross-linked with DSS (Thermo Scientific #88805). They were then incubated with cell lysate overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... blots were incubated with an anti-SARS-CoV spike protein polyclonal antibodies (S1, Sino Biological #40591-T62; S2, Invitrogen #PA1-41165 ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Developmental Biology 2020Quote: ... Anti-mouse VE-cadherin (VC) antibody was biotinylated in-house using FluoReporter Mini-Biotin-XX Protein labeling kit (Invitrogen). Cell populations were sorted using a FACSAria-II sorter (BD ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with either horseradish peroxidase (HRP)-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) or HRP-conjugated anti-GFP antibody (1:5,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by secondary antibody for 1 hour and processed for protein detection using SupersignalTM West Pico PLUS (Thermo Scientific) or Immobilon Western chemiluminescent substrate (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with anti-green fluorescent protein rabbit IgG primary antibody (1:1000; Molecular Probes, OR, USA) and anti-Tyrosine Hydroxylase (TH ...
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bioRxiv - Cancer Biology 2023Quote: ... and MNase treated and antibody bound chromatin collected by adding 25 μl of Dynabeads Protein G (Thermo Fisher Scientific) and rotating for 1 hour at 4°C as previously described (Heinäniemi ...
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bioRxiv - Bioengineering 2023Quote: ... The antibodies were diluted to 0.12 mg/mL and then mixed with Protein Thermal Shift Dye (Applied Biosystems, 4461164) to achieve a final concentration of 1x in final volume of 20 µL ...
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bioRxiv - Immunology 2023Quote: ... Protein detection was performed by incubation with a 6x-His Tag mouse monoclonal antibody (clone: HIS.H8; Thermo Fisher Scientific), followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N ...
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bioRxiv - Neuroscience 2022Quote: ... Sheared chromatin was incubated with anti-CEBPG or anti-rabbit IgG control antibody attached to protein A Dynabeads (Invitrogen) and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin was incubated with antibodies overnight at 4°C and equal amounts of Protein A and G Dynabeads (Invitrogen), pre-blocked with BSA (1 mg/ml) ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The immuno-detection of proteins was carried out using mouse anti-HA monoclonal antibody (2-2.2.14, #26183, ThermoFisher Sci) and secondary anti-mouse IgG antibodies conjugated with horseradish peroxidase (#A9044 ...
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bioRxiv - Bioengineering 2024Quote: ... and the mouse monoclonal antibody (17TF2-1H4) specific for the bacterial maltose-binding protein (anti-MBP, #MA3045 Fisher Scientific) were purified as described,[34] with minor modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed by adding 2 mg of P-CREB antibody to protein A/G Dynabeads (Life Technologies, USA) and incubating the mixture overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression of the TAP-Mga2 protein was verified by western blot with an anti-TAP antibody (Thermo Scientific, CAB1001).
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bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
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bioRxiv - Cell Biology 2024Quote: ... For protein detection secondary anti-mouse or anti-rabbit antibodies conjugated either to Alexa Fluor 488 and 555 (Invitrogen) for immunofluorescence or to horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins transferred to nitrocellulose membranes were blocked and blotted using primary antibodies against: 6xHis (mouse monoclonal, Invitrogen MA1-135); 20S α4 PSMA7 (mouse monoclonal anti-human ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation was performed using 500µg of sheared chromatin lysate and 5µg of HIF-2α antibody (Novus NB100-122) pre-conjugated to protein G beads (Invitrogen). Lysate and antibody-beads were incubated for 16 hours at 4°C with rotation and then washed four times in wash buffer [50mM HEPES-HCl(pH 8) ...
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bioRxiv - Developmental Biology 2024Quote: ... The antibody was added to at least 5μg of sonicated chromatin along with Dynabeads Protein G magnetic beads (Invitrogen) and incubated with rotation at 4°C overnight ...