Labshake search
Citations for Thermo Fisher :
451 - 500 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... The sheared chromatin supernatant was incubated with antibodies bound to G protein beads (Invitrogen). The crosslinks were reversed ...
-
bioRxiv - Genomics 2023Quote: 1 μg of antibody was coupled with 10 μL protein A (Invitrogen, cat# 10002D) and 10 μL protein G (Invitrogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... FLAG-METTL3 variants were immobilized on 25 µL antibody-conjugated protein G dynabeads (Invitrogen) upon expression in HEK293 cells and immunoprecipitation (4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... composed of primary antibodies combined with Protein G Agarose (Thermo Fisher Scientific, Waltham, MA) were collected ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... The clarified supernatant was added to pS6 antibody-loaded beads (Protein A Dynabeads, Invitrogen) and incubated for 10 minutes on an end over end mixer ...
-
bioRxiv - Molecular Biology 2023Quote: ... antibody was purified on Protein A resin from clarified cell supernatant (Thermo Fisher Scientific). Large-scale production of 10-1074 was conducted at Scripps Research Center for Antibody Development & Production (La Jolla ...
-
bioRxiv - Neuroscience 2023Quote: ... 0.5 mM EGTA) using the indicated antibodies with dynabeads protein G (Thermo Fisher Scientific).
-
bioRxiv - Genomics 2022Quote: ... 10 μg of antibody was coupled to 50 μL of Protein A Dynabeads (Invitrogen) and incubated overnight at 4°C prior to ChIP ...
-
bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in I-BlockTM protein-based blocking reagent (Applied Biosystems, Cat.No.T2015).
-
bioRxiv - Neuroscience 2023Quote: ... rat anti-glial fibrillary acidic protein (GFAP) antibody (Thermofisher, Cat# 13-0300, 1:70), and chicken anti-microtubule-associated protein 2 (MAP2 ...
-
bioRxiv - Cell Biology 2023Quote: ... Bead preparation and antibody binding: 30-50 µL of DynabeadsTM Protein G (Invitrogen, 10003D) were used per ChIP ...
-
bioRxiv - Cell Biology 2023Quote: ... All other proteins were detected with secondary antibodies conjugated to DyLight800 (1:10000, ThermoFisher). Images were acquired with a ChemiDoc MP Imaging System (BioRad) ...
-
bioRxiv - Microbiology 2023Quote: ... or no protein in 200 µl antibody binding and washing buffer (Invitrogen kit 10006D) for one hour at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... Chromatin was isolated using a Rad21 antibody (ab992) and Protein-G Dynabeads (Thermo Fisher) and washed three times with RIPA buffer (50 mM HEPES-KOH ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used: RhoA (Invitrogen, #PA5-87403, 1:500), Rac1 (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... KRP3 protein level was detected by western blotting with anti-6xHis antibody (Thermo Fisher).
-
bioRxiv - Cell Biology 2024Quote: Antibodies against the following proteins were used for immunoblotting: β-actin (Life Technologies M4302), TAZ (#2C2C9 ...
-
bioRxiv - Neuroscience 2024Quote: ... Antibody concentration was measured using a Qubit protein assay (Q33211, Life Technologies, Darmstadt, Germany). Antigen specificity was confirmed through cell-based assays in HEK-293T cells expression full-length human CASPR2 as previously described.
-
bioRxiv - Neuroscience 2024Quote: ... followed by incubation with 20 μL Dynabeads Protein A (ThermoFisher, 10007D for rabbit antibody) for 2 h at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... The lysates were immunoprecipitated with antibody conjugated Dynabeads™ Protein G Magnetic Beads (Invitrogen).
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies (5µg) or isotype controls (5µL) were incubated with 40µL protein A (Invitrogen) or protein G (Pierce ...
-
bioRxiv - Genomics 2024Quote: ... Peroxidase-conjugated antibodies were used at the following concentrations: protein A (Life Technologies 101023) 1:4000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus, Thermo Scientific). Antibodies against human CD11b ...
-
bioRxiv - Cell Biology 2021Quote: ... Expression of ectopic proteins was confirmed via western blotting with FHIP1B and FHIP2A-specific antibodies as well as anti-V5-HRP antibody (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... or a major outer membrane protein (MOMP) antibody (1:1000, Novus Bio) and an appropriate AlexaFluor secondary antibody (1:200, ThermoFisher Scientific). Counterstaining was performed with Hoechst stain ...
-
bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher) before neurons were washed 3 times with HBS and mounted on the microscope in HBS ...
-
bioRxiv - Neuroscience 2024Quote: ... on PD7 hippocampal tissue using anti-GFP antibodies (Memorial-Sloan Kettering Antibody and Bioresource Core Facility) and protein A/G magnetic beads (ThermoFisher, 88803). Following the final wash of the RNA-antibody-bead complex ...
-
bioRxiv - Cancer Biology 2024Quote: ... Appropriate primary antibodies and HRP-conjugated secondary antibodies were used and proteins were detected using the Enhanced Chemiluminescent (ECL) reagent (Thermo Scientific). The images were acquired with ChemiDoc MP System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... H3K27Ac and SP1 antibody or control (immunoglobulin G) antibody overnight at 4°C followed by addition of Protein G Dynabeads (Invitrogen, 10004D). The obtained complex and input DNA were decrosslinked in TE buffer with 1% SDS and proteinase K (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... the Ldh-EtfAB complex was stabilized with bis(sulfosuccinimidyl)suberate (BS3) crosslinker (Thermo Fisher Scientific, Waltham, USA) by incubating the sample (≈ 1 mg ...
-
bioRxiv - Genetics 2020Quote: ... and only RPE/choroid/scleral complex was stained with isolectin-B4 (IB4, 10μg/ml, I21413, Life Technologies) overnight ...
-
bioRxiv - Cancer Biology 2020Quote: ... The complex was washed 8-10 times by RIP buffer and the RNA extracted using Trizol (Invitrogen).Then ...
-
bioRxiv - Microbiology 2024Quote: ... The datasets of Csy-DNA complex samples were collected using a Titan Krios microscope (Thermo Fisher Scientific) with K2 Summit direct electron detectors and a GIF Quantum energy filter (Gatan) ...
-
bioRxiv - Biophysics 2023Quote: The YiiP-Fab2r complex was imaged with a Titan Krios G3i electron microscope (Thermo Fisher Scientific, Inc) equipped a Bioquantum energy filter and K2 or K3 direct electron detector (Gatan ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Cryo-EM imaging of the nucleotide-free β2AR-GsEMPTY complex was performed on a Titan Krios (ThermoFisher) electron microscope equipped with a K2 Summit direct electron detector (Gatan ...
-
bioRxiv - Biochemistry 2023Quote: ... Amine-specific crosslinking of histones within the complex was done with bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific). 20-μl reactions containing complex in (20 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2023Quote: ... Brain-ring gland complex were stained overnight at 4ºC with chicken anti-gfp (1/500, Life Technologies), and rat anti-pvr [6] (1/500 ...
-
bioRxiv - Cell Biology 2023Quote: ... The RNP complex was then electroporated into HeLa cells using the Neon NxT Electroporation System (ThermoFisher Inc.) under the kit conditions provided by the manufacturer ...
-
bioRxiv - Cancer Biology 2024Quote: ... The RNP complex was then electroporated into 22Rv1 cells using the Neon NxT Electroporation System (ThermoFisher Inc.) under the kit conditions provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Add 100 µL Pol II antibody-conjugated beads (10 µg Pol II 8WG16 antibody conjugated with Dynabeads™ Protein G for Immunoprecipitation (ThermoFisher, #1004D)) and incubate on a tube rotator for 1 hour in the 4 °C room ...
-
bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
-
bioRxiv - Microbiology 2020Quote: ... were calculated by IFA using primary antibodies against viral proteins and Alexa FluorTM 488 α-rabbit and Alexa FluorTM 586 α-mouse as secondary antibodies (Invitrogen), respectively.
-
bioRxiv - Cancer Biology 2020Quote: ... Chromatin fragments were immunoprecipitated overnight using 1 μg antibody of SOX11-PAb antibody and 20 μl Protein A UltraLink® Resin (Thermo Scientific) beads per 10×106 cells ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with anti-IRE1 antibody or anti-GST antibody and precipitated using protein A-agarose (Invitrogen, Carlsbad, CA, USA). Precipitated proteins were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (rabbit IgG-, Protein A-, or mouse IgG-conjugated with HRP, Thermo Fisher Scientific) and ECL detection reagent (Amersham) ...
-
bioRxiv - Molecular Biology 2024Quote: The following primary antibodies were used for the immunological detection of specific proteins on PVDF membranes: anti-Tap (CAB1001 rabbit polyclonal antibody, Invitrogen, 1:10.000), anti-HA (12CA5 mouse monoclonal antibody ...
-
bioRxiv - Biochemistry 2024Quote: ... cell lysates were prepared as described above and subjected to immunoprecipitation with anti-DYKDDDDK-tag antibody clone 1E6 or mouse IgG anti-HA antibody clone 4B2 using Protein G Dynabeads (Thermo Fisher Scientific). The beads (bound fraction ...
-
bioRxiv - Cancer Biology 2024Quote: ... Chromatin from each sample was then immunoprecipitated overnight with 5μg of the following antibodies (per sample) premixed with Protein G Dynabeads (for mouse antibodies; Thermo Fisher Scientific, 10004D) or Protein A Dynabeads (for rabbit antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... A ChIP-grade antibody was incubated with 30 μl of protein G Dynabeads (Thermo Fisher) for 4 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies included rabbit polyclonal anti-β-COP protein (1:200; Thermo Fisher, PA1-061) and mouse monoclonal anti-flag (1:500 ...