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Citations for Thermo Fisher :
6601 - 6650 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: SDS-PAGE gels were transferred onto a nitrocellulose membrane using an iBlot 2 Dry Blotting System (Thermo Fisher), blocked with 3% powdered milk in TBS-T and then incubated at 4°C overnight with primary antibodies diluted in 3% powdered milk or 3% BSA ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were loaded onto a NuPAGE 4-12% Bis-Tris pre-cast SDS gradient gel (Thermo Fisher Scientific), transferred to a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates were run onto NuPAGE®4–12% Bis-Tris Gels (Novex Life Technologies, Waltham, MA) and transferred onto PVDF membrane using the Bio-Rad transfer system ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE on 10% Bolt Bis-Tris gels (catalog number NW00105BOX, Thermo Fisher Scientific), then transferred to 0.45 micron PVDF membranes (catalog number IPVH00010 ...
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bioRxiv - Cancer Biology 2022Quote: ... Twenty micrograms of protein were loaded in each lane and run on 10-20% tris glycine gels (Invitrogen). Gels were transferred to PVDF membranes (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... 6.6 mM EDTA) and analysed with electrophoresis on a 0.5 % agarose gel containing SYBR Green I (Thermo Fisher) for 50 min at 70 V.
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bioRxiv - Cell Biology 2022Quote: ... Sample buffer was added and the proteins resolved by electrophoresis on a NuPAGE 10% BisTris gel (Thermo Fisher). The gel was stained using SimplyBlue Safestain (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The heated samples were briefly centrifuged at high speed before being resolved on 4-12% NuPAGE gel (Invitrogen). Proteins were transferred onto a PVDF membrane filter paper sandwich (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Samples were either run on 4-12% NuPAGE or Bolt gels with the NuPAGE Bis-Tris system (Invitrogen) in MOPS SDS buffer or on 8% gels with the BIO-RAD system with SDS-running buffer ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments of expected size were stained with SYBR Safe DNA gel stain (Thermo Fisher Scientific, Waltham, MA) on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No ...
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bioRxiv - Immunology 2022Quote: ... heated at 95°C for 5min and separated on a 10% Bis-Tris gel (Cat. No. WG1203BOX, Invitrogen). Silver staining was performed using the PierceTM silver stain kit (Cat ...
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bioRxiv - Microbiology 2022Quote: ... Eluted protein complexes were separated by one-dimensional 4-12% NuPage Novex Bis-Tris Gel (Invitrogen, Cat. NP0321BOX) and stained using the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were analyzed by agarose gel electrophoresis and purified using the GeneJET PCR purification kit (Thermo Fisher) per the manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: Equivalent amounts of total proteins were run in Bolt 4%-12% Bis-Tris gradient gels (Thermo Fisher Scientific) or 15% tris-glycine-SDS acrylamide gels and transferred to 0.2 μm-pore size nitrocellulose membranes in Towbin buffer (25 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Western blots were performed as we previously described using NuPAGE™ 10% Bis-Tris Protein Gels (Life Technologies). The primary anti-tau antibodies used are listed in Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... S6 and subjected for BN-PAGE electrophoresis using the NativePAGE Novex 3–12% Bis-Tris Protein Gels (Invitrogen), following the protocol provided by Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... the integrity and quantity of RNA sample using agarose gel electrophoresis and NanoDrop ND-1000 (Thermo Scientific, USA). RNA sample were sequenced by Illumina HiSeq 2500 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Seventy µg of tissue homogenate was resolved on 10-20% Novex Tris-Glycine Mini Protein Gel (Thermo Fisher) for 20 min at 75 V ...
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bioRxiv - Molecular Biology 2022Quote: ... 20μg total protein were loaded on SDS-PAGE precast gradient gels (4–12% Nu-Page Bis-Tris, Invitrogen), followed by transfer to nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: ... DNA quality was verified with 1% agarose gel electrophoresis and spectrophotometry (NanoDrop 2000, Thermo Fisher Scientific, MA, USA). DNA concentration was determined with the Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants from cell lysates were run on 4–12% NuPAGE Bis Tris precast gels (Life Technologies, Beverley, MA) using MES running buffer (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... Western blotting was performed with whole-cell lysates run on 4-12% Bis-Tris NuPage gels (Life Technologies) and transferred onto Immobilon-P Transfer Membranes (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were loaded onto a 0.8% agarose gel stained with a 1/10,000 dilution of SYBR gold (Invitrogen) and visualized using a ChemiDoc MP imaging system (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDS-PAGE electrophoresis on NuPAGE™ 4-12% Bis-Tris gels (Thermo Fisher Scientific) and MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were heat denatured and separated on 4–12% Bolt Bis-Tris gels (Thermo Fisher Scientific: NW04125BOX). The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... Products were resolved by 1.5% agarose gel electrophoresis and visualized by staining with SYBR Safe (Thermo Fisher Scientific). We verified that splicing was not a result of residual DNA excision products being formed in cells (e.g ...
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bioRxiv - Microbiology 2024Quote: ... Samples of the elution fractions were analyzed by SDS-PAGE on a 4-12% Bis-Tris gel (Invitrogen) to check the presence and purity of the protein ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-30 µg of lysates were resolved on pre-cast 10% or 12% Bis-Tris NuPAGE gels (Invitrogen), transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... RNA yield and integrity was verified using a NanoDrop spectrophotometer and a Novex 6% TBE-urea gel (Invitrogen), respectively.
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bioRxiv - Cell Biology 2024Quote: ... They were then resolved by SDS-PAGE electrophoresis using bis-tris NuPage gels as per manufacturer’s instructions (Invitrogen) and transferred to PVDF membranes which was blocked using 3% BSA dissolved in TBST (50 mM Tris [pH8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was run at 120 V (constant) for 60 minutes in 1X MOPS SDS running buffer (ThermoFisher) using the Bolt Mini Blot Module (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Genetics 2023Quote: ... Small RNA was resolved on a 12.5% denaturing PAGE 7M-Urea gel and stained with SYBR-gold (Invitrogen). For cloning ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
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bioRxiv - Microbiology 2024Quote: ... The extracted proteins were stacked in the top of an SDS-PAGE gel (4-12% NuPAGE, Life Technologies), stained with Coomassie blue R-250 (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... and equivalent concentrations (10-16ug) loaded per sample on precast NuPAGE 4-12% Bis-Tris gradient gels (Invitrogen). Separated proteins were transferred to nitrocellulose and probed overnight with primary antibodies under agitation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen, Cat. No. EC6265BOX) to retain only the small RNA inserts ...
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bioRxiv - Genetics 2024Quote: ... 20 μg protein was loaded per sample into 15-well Novex WedgeWell Tris-Glycine Mini gels (Invitrogen #XP08165BOX). Gels were run at 120 V for 90 minutes and then transferred onto 0.45 μm nitrocellulose membranes (Cytiva #10600002 ...
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bioRxiv - Immunology 2024Quote: ... Coomassie G-250 additive was added to samples and loaded onto NativePAGE 3-12% Bis-Tris gels (Invitrogen). Samples were first run in dark blue cathode buffer (inner chamber ...
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bioRxiv - Genomics 2024Quote: ... Equal protein amounts from each fraction were run on 4-12% bis-tris plus acrylamide gels (Invitrogen, NW04122) and then immunoblotted ...
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bioRxiv - Immunology 2024Quote: ... Coomassie G-250 additive was added to samples and loaded onto NativePAGE 3-12% Bis-Tris gels (Invitrogen). Samples were first run in dark blue cathode buffer (inner chamber ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplicons were visualized with SYBR Safe DNA Gel Stain in 2% Ultra Pure Agarose (Thermo Scientific, USA). DNA bands were excised and purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... vortexed and loaded onto a 15% pre-cast Novex Urea-TBE gel (Thermo Fisher Scientific Corp, Waltham MA) and run at 180V for 60 minutes alongside Small RNA Marker (Ab Nova GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... equal volumes of viral lysates separated by electrophoresis on Bolt 4–12% Bis-Tris Plus gels (Life Technologies) were transferred using Trans-Blo Turbo Mini PVDF Transfer Packs (Bio-Rad) ...
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bioRxiv - Biophysics 2024Quote: ... Protein samples were separated using pre-cast 4-12% Bis Tris Protein Gels (Thermo-Fisher Scientific, Waltham MA) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... All PCR products were resolved on 2% agarose gel in 1xTAE buffer with a 1kB Plus Ladder (Invitrogen). Primers to detect splice variants are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of sEVs separated by SDS-PAGE gels were transferred to polyvinylidene difluoride membrane (PVDF; ThermoFisher Scientific) for 1 h at 100 V ...
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bioRxiv - Immunology 2023Quote: Blue Native polyacrylamide gel electrophoresis (BN-PAGE) was performed using Bis-Tris NativePAGE system (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions ...