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Citations for Thermo Fisher :
7001 - 7050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... BMDMs were cultured with 1:1000 SYTOX™ Green Ready Flow™ Reagent (Invitrogen, R37168) in the IncuCyte ZOOM® incubator in a 96-well plate from day 6 on ...
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bioRxiv - Biophysics 2024Quote: ... imager strands were diluted in 1x phosphate buffered saline (PBS, diluted from 10x stock, #14200067, Gibco, Thermo Fisher Scientific, USA), 0.5 M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... penicillin/streptomycin (Thermo Fisher Scientific, Carlsbad, CA), and supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2024Quote: ... The unbound linker was removed using Zeba desalting columns (Thermo Fisher Scientific, Germany). Azide-modified P1 docking strand and linker-antibody conjugate were incubated in a 10:1 molar ratio overnight at 4°C while slightly shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... the maximum lysis was observed in the treatment with 5% SDS Solution (Thermo Fisher Scientific, Carlsbad, CA).
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bioRxiv - Cancer Biology 2024Quote: ... T cells were stimulated for 5 days with Dynabeads Human T-Activator CD3/CD28 (Life Technologies, Carlsbad, CA) at a bead-to-cell ratio of 3:1 ...
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bioRxiv - Biophysics 2024Quote: ... and latrunculin B were obtained from Thermo Fisher Scientific and used without further purification ...
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bioRxiv - Cancer Biology 2024Quote: ... with TaqManTM Universal MasterMix II (Thermo Fisher, 4440044) and LightCycler® 480 II (Roche) ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 1% penicillin/streptomycin (Gibco, Billings, MT, USA), 1 µM A83-01 (Tocris ...
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bioRxiv - Cancer Biology 2024Quote: ... serum-free (Gibco) and penicillin-streptomycin (Gibco) ...
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bioRxiv - Biophysics 2024Quote: ... in a Qubit 4.0 Fluorometer (Life technologies). Equimolar amounts of indexed libraries were pooled and sequenced on a NextSeq 2000 (Illumina) ...
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bioRxiv - Biophysics 2024Quote: ... and mounted with ProLong Gold Antifade (Thermo Fisher Scientific). SIM images were acquired using an ELYRA 7 LS mit 2x pco.EDGE 4.2 (Zeiss ...
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bioRxiv - Biophysics 2024Quote: ... slides were incubated for 1 h at RT in a humid chamber with secondary antibodies in PBS-T and 2% milk: 1:1,000 goat anti-mouse Alexa Fluor 488 (A11001; Invitrogen) and 1:1,000 donkey anti-rabbit Alexa Fluor 555 (A31572 ...
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bioRxiv - Biophysics 2024Quote: ... without or with the presence of ligands (PIPA 200 μM, HOCPCA 1000 μM, 5-HDC 100 μM) using Zeba™ Spin Desalting Columns (Thermo Fisher Scientific, 0.5 ml 7K MWCO). Dilution series were made of CaMKIIα WT hub ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (#15140122, Invitrogen), in a humidified 5% CO2 atmosphere at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Mg2+-free (Gibco), on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... and penicillin-streptomycin (Gibco). Cell disaggregation was done with a polished pipet and the cell suspension was filtered with 40 μm cell strainer (Corning) ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney (HEK) 293T (#CRL-3216, ATCC) cells were maintained in DMEM GlutaMAX medium (#61965026, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (#15140122 ...
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bioRxiv - Biophysics 2024Quote: ... Each sample was prepared with a final concentration of 0.1 mg/mL CaMKIIα and 8x SYPRO® Orange Protein Gel Stain (Life Technologies, #S6650) in MES buffer (20 mM MES ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg/ml anti-CD3 (Thermo Fisher Scientific, 16-0032-82) and 5 μg/ ml anti-CD28 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... and 1:1,000 donkey anti-rabbit Alexa Fluor 555 (A31572; Invitrogen). DNA was counterstained with DAPI (1:2,000 ...
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bioRxiv - Biophysics 2024Quote: Cells were cultured in either RPMI with L-glutamine (FisherScientific, cat# 12004997)—for suspension cells—or DMEM with high glucose (ThermoFisher, cat# 11965092)—for adherent cells—supplemented with 10% Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... excess dye was cleared via use of Zeba desalting columns (Thermo Fisher Scientific, Waltham, Massachusetts).
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bioRxiv - Biophysics 2024Quote: ... with CaMKIIα holoenzyme (#PR4586C, Thermo Fisher). Kinase detection reagent and the ADP-Glo kinase reaction buffer (40 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... and 100 μg/mL streptomycin and 1%(v/v) GlutaMAX (all reagents from Gibco, Thermo Fisher, Germany). Both cell lines were incubated at 37°C and 5% CO2 in an automatic CO2 incubator (Model C150 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 μg/ ml anti-CD28 (Thermo Fisher Scientific, 16-0281-82) antibodies were added to 2×106 T cells/ ml in RPMI medium ...
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bioRxiv - Cancer Biology 2024Quote: ... The DRGs were immersed in 10 mM HEPES (Gibco) solution in HBSS Ca+ ...
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bioRxiv - Biophysics 2024Quote: ... and subsequently dialyzed with a 3.5 kDa molecular weight cut-off (m.w.c.o.) membrane (SnakeSkin, Thermo Scientific) against the final reaction buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... The protein-dye solution was then added to two consecutive 0.5 mL 40K MWCO Zeba™ Spin Desalting Columns (ThermoFisher, cat# 87767) (previously washed twice with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Live/Dead Fixable Aqua Dead Cell Viability Reagent (405 nm excitation – Invitrogen - L34957), anti-TCR-Ɣδ (clone B1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibodies included NINJ1 (1:1000, Invitrogen, PA5-95755), GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The culture media used were Dulbecco’s Modified Eagle Medium (DMEM) (GIBCO-11995) for HT-1080 ...
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bioRxiv - Biophysics 2024Quote: ... Cryo-EM samples were frozen using a Vitrobot Mark IV (Thermo Fisher Scientific) maintained at 4°C and 100 % humidity ...
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bioRxiv - Biophysics 2024Quote: ... All probes used for real-time PCR (Applied Biosystems, Carlsbad, CA, USA) are summarized in Table 1 ...
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bioRxiv - Biophysics 2024Quote: ... with NuPAGE MOPS SDS Buffer (Invitrogen) and running at 80 V for 15 minutes followed by 200 V for 45 minutes on ice ...
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bioRxiv - Biophysics 2024Quote: Cells were freeze-thawed and further lysed in RIPA (radioimmunoprecipitation) lysis buffer (Thermo Fisher, Waltham, MA, USA) supplemented with protease and phosphatase inhibitor cocktail (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... with NativePAGE Running Buffer and NativePAGE Cathode Buffer in the inner chamber (Invitrogen) before running at 150 V for 1 hour followed by 250 V for 1 hour on ice ...
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bioRxiv - Biophysics 2024Quote: ... and the proteins were transferred to PVDF membrane (Thermo Fisher). The membrane was blocked in 10% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2024Quote: ... Following in vitro transcription to cRNA was performed using the linearized cDNA template and the mMessage mMachine® T7 transcription kit (Ambion, Thermo Fisher Scientific, Waltham, MA). For cRNA purification the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Biophysics 2024Quote: ... Total protein concentration was determined with the BCA assay (Thermo Fisher, Waltham, MA, USA). Samples containing 20 µg of protein were separated on SDS-polyacrylamide gels and transferred to PVDF or NC membranes (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... and other consumables were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biophysics 2024Quote: ... 1µg peptides from each sample was introduced to MS using the Vanquish Neo (Thermo Scientific). The trapping column was PEPMAP NEO C18 (5 µm particle size ...
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bioRxiv - Biophysics 2024Quote: ... EU labeling of RNAs was detected using the Click-iT RNA Imaging Kit (Life Technologies, cat. #C10639). HPG labeling of proteins was detected using the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit (Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... The trapping column was PEPMAP NEO C18 (5 µm particle size, 300 µm*5mm, Thermo Scientific). Analytical column was nano EaseTM M/Z HSS C18 T3 (100Å ...
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bioRxiv - Biophysics 2024Quote: ... TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA, USA) were used ...
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bioRxiv - Biophysics 2024Quote: ... and 1% penicillin/streptomycin (Gibco, #15-140-122) at 37°C in 5% CO2-95% air.
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bioRxiv - Biophysics 2024Quote: ... The reaction was resolved on a 4-20% gradient SDS-PAGE gel (Invitrogen), and the proteins were transferred to PVDF membrane (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: ... treated with a 1:1 ratio of 0.04% trypan blue (Gibco), and counted using a hemocytometer ...
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bioRxiv - Biophysics 2024Quote: ... 10% acetic acid microwaved for 1 minute and then stained with Colloidal Blue Stain (Invitrogen) overnight and de-stained in water and scanned the following day ...
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bioRxiv - Biophysics 2024Quote: ... and incubated at 80°C for 10 minutes before loading into 4-12% Bis-Tris gel (Invitrogen) with NuPAGE MOPS SDS Buffer (Invitrogen ...