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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Immunoprecipitation of ribosomes was carried out by incubation of the supernatant with DynabeadsTM Protein G (Invitrogen) and anti-GFP monoclonal antibodies (19C8 and 19F7 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... negative control sequences (see Table below) were designed from pcDNA6.2-GW/EmGFP-miR-neg Control (Invitrogen, Carlsbad, CA, USA). For construction of pAAV-CMV-EmGFP-miRNA-S100a10 or Htr1b ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... qPCR was performed using the StepOne real-time PCR system (Life Technologies, Carlsbad, CA, USA). The final reaction volume was 10□μl (5□ng of cDNA with primers and Thunderbird SYBR qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-rabbit-Alexa647 (1:333, Invitrogen, #A-21245), goat anti-guinea pig Alexa488 (1:333 ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-guinea pig Alexa647 (1:333, Invitrogen, #A21450), HRP-linked horse anti-mouse IgG (1:10,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-guinea pig Alexa488 (1:333, Invitrogen, #A11073), goat anti-guinea pig Alexa568 (1:333 ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-guinea pig Alexa568 (1:333, Invitrogen, #A11075), goat anti-guinea pig Alexa647 (1:333 ...
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bioRxiv - Developmental Biology 2024Quote: ... The main olfactory epithelia were dissected from the bone under a dissection stereo microscope and quickly immersed in ice-cold Hank’s Balanced Salt Solution (HBSS, Gibco, #14175103). Each epithelium was then washed once with HBSS and resuspended in Earle’s Balanced Salt Solution (EBSS ...
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bioRxiv - Developmental Biology 2024Quote: ... serum-free B-27 (Gibco, #17504044), L-glutamine (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: Cultured HEK293 cells were grown on 12 mm round cover glasses (Azer Scientific, #200121) pre-coated with poly-D-lysine (Gibco, #A3890401) in 24-well plates and then transfected with a plasmid encoding HA-tagged neurexin1 alpha using lipofectamine 2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... using a NanoDrop One Spectrophotometer (ThermoFisher Scientific). Equal amounts of protein (0.25 µg ...
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bioRxiv - Developmental Biology 2024Quote: ... Following a standardized overnight automated processing protocol (Thermo Scientific, Excelsior ES), the samples were embedded in paraffin ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Developmental Biology 2024Quote: ... The gels were transferred onto PVDF membranes using iBlot PVDF gel transfer (ThermoFisher Scientific). The membranes were then immersed in a blocking solution (5% nonfat milk in TBST ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the lungs were flushed twice with 1-ml sterile BAL buffer (PBS (Gibco), 2 nM EDTA (Corning ...
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bioRxiv - Plant Biology 2024Quote: ... The extracts were analyzed using a UPLC-APCI-MS/MS system (UPLC, ExionLC™ AD, https://sciex.com.cn/; MS, Applied Biosystems 6500 Triple Quadrupole ...
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bioRxiv - Plant Biology 2024Quote: ... Trapping was performed at 20 μl/min for 2 min in loading solvent A on a 5 mm trapping column (Thermo scientific, 300 μm internal diameter (I.D.), 5 μm beads) ...
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bioRxiv - Plant Biology 2024Quote: ... The AvrStb61E4 coding sequence lacking its endogenous signal peptide was amplified from 1E4 cDNA and pPICZalphaB (Invitrogen) was linearized using an inverse PCR approach ...
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bioRxiv - Plant Biology 2024Quote: ... the extract was filtered through a 0.22 μm membrane and analyzed using a UPLC-ESI- MS/MS system (UPLC, Shimadzu Nexera X2, https://www.shimadzu.com.cn/; MS, Applied Biosystems 6500 QTRAP ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 1% GlutaMAX (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... HEK293T (human embryonic kidney 293T) cells were incubated in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... all medium was aspirated and replaced with 1 mL DMEM without glutamine (Gibco) supplemented with 10% FBS and 1% GlutaMAX ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA was extracted from the flow-through using Trizol (Thermo Fisher, 15596018CN). The ribosomal RNA fragments were removed using the Ribo-off rRNA depletion kit (Plant ...
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bioRxiv - Plant Biology 2024Quote: ... using 1 μg of total RNA and the Verso cDNA synthesis kit (Thermo Fisher Scientific, United States). The synthesized cDNAs were then diluted 10 times (V/V ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized using SuperScript III Reverse Transcriptase (Invitrogen). One reaction was done in total volume of 15 μl including ...
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bioRxiv - Plant Biology 2024Quote: ... The quality and quantity of the RNA samples were analysed by agarose gel electrophoresis and Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized and cloned into pMS (GeneArt, Thermo Fisher Scientific, Waltham, USA).
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA). For each sample ...
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bioRxiv - Plant Biology 2024Quote: ... 0.6 μL MgCl2 (1.5 mM, Thermo Fisher, Cat. No. R0971) and 13.08 μL ddH2O ...
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bioRxiv - Plant Biology 2024Quote: ... reverse transcription was performed using 3ug RNA and the SuperScript IV reverse transcriptase first-strand synthesis kit (Invitrogen). PCR programs were run on an Applied Biosystems 96-Well Thermal Cycler (Eppendorf AG ...
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bioRxiv - Plant Biology 2024Quote: ... the promoter regions upstream of the start codon of DGK1 (2472 bp) and DGK2 (1438 bp) were inserted via BP reactions (Invitrogen) into a pDONR R4-L1 vector ...
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bioRxiv - Plant Biology 2024Quote: ... The blots were washed and applied to chemiluminescent nucleic acid detection module (Thermo Fisher, 89880). then signals were detected by Chemiluminescence imaging ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) containing 1% penicillin/streptomycin (P/S ...
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bioRxiv - Plant Biology 2024Quote: ... using a homemade Pfux7 polymerase (Nørholm 2010) with the Phusion Green HC Buffer (F538L, Thermo Fisher). Primer sequences are listed in Supp ...
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bioRxiv - Biochemistry 2024Quote: ... 100 U/ml penicillin and 100 μg/ml streptomycin (15140-122, penicillin-streptomycin, Thermo Fisher Scientific, Waltham, MA). Namalwa cells were cultured in suspension in RPMI medium (Sigma-Aldrich ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... We used Alexa Fluor 594 goat anti-mouse IgG (A11005, Invitrogen, 1:250 dilution in PBS) to stain CaMKIIα for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was eluted with 100% Buffer B and dialyzed overnight using Slide-a-lyzer (ThermoFisher) cassettes in dialysis buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Reagents were dispensed into assay-ready plates using a Combi Multidrop (Thermo Scientific). Fluorescence was measured at 685 nm using a PerkinElmer Envision 2105 plate reader ...
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bioRxiv - Biochemistry 2024Quote: A DTNB solution containing 50 μM DTNB (ThermoFisher) and 200 μM TCEP (Hampton Research ...
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bioRxiv - Biochemistry 2024Quote: The samples were analyzed by LC/MS/MS using an Easy nLC 1200 coupled to a QExactive HF mass spectrometer (Thermo Scientific). Samples were injected onto an Easy Spray PepMap C18 column (75 μm id × 25 cm ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were injected onto an Easy Spray PepMap C18 column (75 μm id × 25 cm, 2 μm particle size) (Thermo Scientific) and separated over a 120 min method ...
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bioRxiv - Biochemistry 2024Quote: Raw data were processed using Proteome Discoverer (Thermo Scientific, version 2.5). For the in-vitro analysis ...
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bioRxiv - Biochemistry 2024Quote: ... The generation of recombinant bacmids and viruses for the production of proteins in the Sf9 system followed the Bac–to–Bac baculovirus expression system protocol (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2024Quote: ... oligonucleotides labeled with biotin by Biotin 3’ DNA labeling kit (Thermo Fisher, 89818) were used to detect precursor rRNA ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with glutamine and 10% FBS (GIBCO, #2526728RP).
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed on an ABI 7500 Fast PCR System (ThermoFisher Scientific, Waltham, MA, USA), using the 2X PowerUp SYBR green master mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... 10ug of RNA was subjected to sequential DNase treatment using the TURBO DNA-free™ Kit (Ambion). Following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from 100 ng of total RNA using SuperScript II (Invitrogen). After RT-PCR with primers surrounding editing sites ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s protocol ...