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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Genetics 2023Quote: RT-qPCR experiments were performed as previously described (Coutton et al., 2019) with cDNAs extracted from various human tissues purchased from Life Technologies® ...
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bioRxiv - Bioengineering 2023Quote: The microdevices containing normal human lung fibroblasts with or without macrophages were fixed using a solution of 2% glutaraldehyde (ACROS organics) in PBS (containing Ca2+/Mg2+ ...
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bioRxiv - Immunology 2023Quote: ... or with rabbit-anti-human TG3 (PA596450, Thermo Fischer Scientific) followed by detection with Alexa 488 donkey anti-rabbit (Molecular Probes) antibody ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Cancer Biology 2023Quote: ... human NRP-1 (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 using an Ab labeling kit (Molecular Probes, A20186). Images were acquired with an LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Cell Biology 2023Quote: The C28/I2 human chondrocytes were cultured in T-75 flasks with Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen Corp., Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Microbiology 2023Quote: ... (74) were cultured in keratinocyte serum-free media (KSFM) supplemented with bovine pituitary extract and human epidermal growth factor (Thermo Fisher). HCT116 human colon carcinoma lines were obtained from ATCC and maintained following their recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... harboring a human APP transgene with the K670N and M671L Swedish mutations (25) were grown in DMEM/F12 medium (Thermo Scientific) supplemented with 5% FBS ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Bioengineering 2023Quote: ... wells were washed 3 times with PBS with 0.1% tween (PBST) and then incubated overnight at 4 °C with a human hemoglobin-FITC conjugated antibody (Life Technologies, A80134F). Samples were washed three times with PBST prior to imaging on a Keyence BZ-X800 microscope.
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bioRxiv - Cell Biology 2023Quote: ... expressing GFP-LC3A (human), were prepared as described previously (Fletcher et al., 2018; Jacquin et al., 2017) and cultured in DMEM/F12 (Gibco, 11320074) containing 5% horse serum (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Neuroscience 2022Quote: ... Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Previously homogenized human-derived AD crude brain extracts were diluted 1 to 20 with Opti-MEM (Thermo Fisher Scientific, cat. 31985062) and sonicated in a Qsonica multiplate horn water bath for 3 minutes at 40% power ...
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bioRxiv - Immunology 2023Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... human prostate tissues were first digested in aDMEM/F12/Collagenase II (1.5mg/ml)/Hyaluronidase VIII (1000 u/ml; Thermo Fisher Scientific) plus 10 μM Y-27632 (Tocris ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Human liver homogenate was prepared from human FLC or matched normal liver tissue (adjacent to the FLC) using RIPA Lysis buffer (Thermo Fisher) with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... 25 nM) or Human YAP/TAZ-siRNA (UGUGGAUGAGAUGGAUACA) (102) by using Lipofectamine RNAiMAX reagent (13778075, Thermo Fisher Scientific, Waltham, MA, USA). Cells were harvested after 72 h.
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bioRxiv - Microbiology 2023Quote: ... The experiments were normalized using human β-Actin as the endogenous control (FAM/MGB probe, cat. no. 401846; Applied Biosystems, USA).
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and dilutions were used: Alexa Fluor 568- and 647-conjugated goat anti-human and anti-rabbit (Life Technologies), 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... we used quantitative TaqMan based OpenArray Human MicroRNA Panel (Cat# 4470187; Thermo Fisher Scientific, Life Technologies Corporation, Grand Island, New York). This panel contains 754 well-characterized human miRNA sequences from the Sanger miRBase v14 ...
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bioRxiv - Systems Biology 2023Quote: ... IgG1 and IgG4 in the basolateral media were quantified using human ELISA) IgG1 and IgG4 quantification kits (Invitrogen, EHIGG1 and BMS2095) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: 100 ng total RNA was reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression Kit following the manufacturer’s protocol (Thermo Fisher Scientific). Target genes (SQSTM1 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and processed for quantitative PCR analysis of SMAD3 and SP1 binding to the human MCEMP1 promoter using Power SYBR Green PCR Master Mix (ThermoFisher Scientific). ChIP-qPCR results were calculated using the ΔΔCt method and are shown as percentage of input DNA for each ChIP experiment.
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was isolated from tissue lysates as well as from cultured human and murine aortic or DA SMCs with the PureLink RNA Mini Kit (ThermoFisher, 12183018). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Cell line derived from human neuroblastoma (SH-EP; CVCL_RR78) was verified by STR profiling and grown in RPMI-1640 (Thermo Fisher Scientific). Murine neuroblastoma cells (NHO2A ...
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bioRxiv - Immunology 2024Quote: ... tissue samples with small volumes (such as human samples) were then included in 1.5% agarose (Invitrogen, Carlsbad, CA, USA; #16500-500). Tissue clearing was then carried out with MetOH dehydration (as described above ...
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bioRxiv - Biochemistry 2024Quote: Human liver specimens were minced to extract genomic DNAs (gDNAs) using the PureLink™ Genomic DNA Mini Kit (Invitrogen™, K182001), and genomic DNA concentrations were measured using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Bioengineering 2024Quote: ... Human dermal fibroblasts (ZenBio, Cat. # DF-F) were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Cat. # SH30021FS) with 10% exosome-depleted FBS (Bio-Techne ...
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bioRxiv - Cell Biology 2024Quote: IMR-90 human fibroblast was purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
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bioRxiv - Microbiology 2024Quote: MRC-5 human embryonic lung fibroblasts were purchased from ATCC (Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco Thermo Fisher) supplemented with 10% FBS (Gibco Thermo Fisher) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...