Labshake search
Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human cMyc-mGlu1 cDNA sequence was subsequently transferred from pcDNA3.1(+) into the destination vector pcDNA5/FRT/TO (Invitrogen) using NEBuilder HiFi assembly (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... 30 nM of siRNA was transfected into the human macrophages using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The human KIF1A coding sequence (DNASU Plasmid Repository, #HsCD00829423, NCBI Nucleotide reference NM_001244008.2) was inserted by Gateway recombination (Invitrogen) to make an in-frame KIF1A-EmGFP fusion.
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bioRxiv - Neuroscience 2023Quote: ... HPCs in suspension were transferred to plates containing an adherent layer of confluent primary human astrocytes (ThermoFisher, N7805100), in media containing Iscove’s Modified Dulbecco’s Medium (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: CEM were counted and resuspended at a concentration of 2-3 x 106 cells/mL in ice cold FACS buffer (PBS, 10% FCS, 2mM EDTA) containing 1.25 µL anti-human CD71 (0.625 µg/mL, OKT9, Invitrogen) for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck) ...
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bioRxiv - Neuroscience 2023Quote: Total tau content was quantified in brain extracts by ELISA (Human Tau (total) ELISA kit (#KHB0041, Invitrogen, UK), see supplementary methods ...
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bioRxiv - Immunology 2023Quote: ... candidate regulatory elements (RE) were PCR amplified from human or mouse genomic DNAwith Accuprime Pfx DNA polymerase (Invitrogen) using primers with a NheI or XhoI cut site added to their 5′ end (primer sequences available in Table S3) ...
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bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...
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bioRxiv - Cell Biology 2022Quote: MS/MS spectra were searched against human database from Uniprot (downloaded on 04/2015) with Proteome Discoverer 2.1.1.21 (ThermoFisher Scientific). Methionine oxidation (+15.9949 Da) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA extraction was performed from the medial layer of the aorta and subsequently sequenced by HTA 2.0 (Human Transcriptome Array 2.0 - Affymetrix) platform.
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Developmental Biology 2023Quote: Human ESCs were thawed and maintained on mitotically inactivated MEFs in Knock-Out DMEM media (Thermo Fisher Scientific) supplemented with 20% Knock-Out Serum Replacement (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma insulin concentration was determined using a Human Insulin ELISA kit according to the manufacturer’s protocol (Invitrogen). The relative bioavailability (RA ...
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Microbiology 2022Quote: ... TNF-alpha treatments were performed for 45 min using 25 ng/ml of human TNF-alpha (Thermo Fisher). Cells were permeabilized with 0.1% Triton X in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma gondii strain RH (expressing YFP off of the GRA1 promoter) (18) was cultured in human foreskin fibroblast (HFF) in complete Dulbeccòs modified Eagle’s medium (cDMEM; ThermoFisher Scientific plus 100 U/ml penicillin/streptomycin ...
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bioRxiv - Cell Biology 2023Quote: Primary human parenchymal lung fibroblasts from non-COPD and COPD patients (n=8) were grown in DMEM (Gibco) supplemented with 5% FBS and 2% antibiotics at 37°C and 5% CO2 ...
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bioRxiv - Genetics 2023Quote: ... the pseudo- haploid calling was carried out on the 593,124 autosomal sites on the Human Origins Array (Affymetrix). Contamination was estimated based on the X-chromosome and mitochondrial DNA using ANGSD(86 ...
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bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and the top 14-HAP depleted and fractionated sample were searched for peptides through the human UniProtKB/SwissProt database (v2021-11-30, 20,306 canonical sequences) using Proteome Discoverer (version 2.5.0.400, Thermo Fisher Scientific). The parameters applied to the search engines Sequest HT and Mascot were set as follows ...
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bioRxiv - Immunology 2023Quote: ... human samples were stained with eBioscience™ Fixability Viability Dye eFluor™ 780 (65-0865-18, ThermoFisher, Canada), while murine samples were stained with Zombie Green™ Fixable Viability Dye (423111) ...
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bioRxiv - Biophysics 2023Quote: ... 77,897 human protein sequences) using the Sequest search engines within the Proteome Discoverer platform (version 2.4, Thermo Scientific). The following search parameters were used ...
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bioRxiv - Developmental Biology 2023Quote: The human Ishikawa were grown in flasks in Modified Eagle’s Medium (MEM, Gibco, Thermo Fisher Scientific, ref. 10370021), supplemented with 5% fetal bovine serum (Biowest ...
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bioRxiv - Biophysics 2024Quote: ... human and primary mouse myotubes were maintained in a mixture of DMEM/F-12 (Gibco: Waltham, MA, USA) containing 20% FBS (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in a 1:1:1:1 ratio of human receptor:Gαq-Rluc8:β3:GFP2-γ9 DNA constructs prepared in Opti-MEM (Invitrogen) using a 3:1 ratio of TransIT-2020 (Mirus Bio ...
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bioRxiv - Immunology 2024Quote: ... incubated for 1h with either horseradish peroxidase (HRP)-conjugated goat anti-human IgM or IgG (Invitrogen, Camarillo, CA), washed again ...
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... and anti-human IgG conjugated to Alexa 488 and Alexa 555 were purchased from Invitrogen (Thermo Fisher Scientific). The Golgi (RCAS1 ...
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bioRxiv - Systems Biology 2024Quote: 150 human islets were hand-picked on the day of distribution and placed in 1ml of Trizol (Invitrogen), vortexed and stored at −80°C until shipping to Oxford or Stanford for further processing ...
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bioRxiv - Physiology 2024Quote: Amyloid-β 40 (Aβ40) in the brain was quantified using a human Aβ40 ELISA kit (Invitrogen, USA, #KHB3482) in accordance with manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: Human iPSCs from the WTC11 background were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401). Human iPSCs from the GIH6C1 background were cultured in mTeSR Plus medium (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: Plasmodium falciparum parasites were grown in human erythrocytes (Stanford Blood Center) at 2% hematocrit in RPMI 1640 media (Gibco) supplemented with 0.25% Albumax II (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Genetics 2021Quote: ... Fetal cortex samples were acquired from the MRC-Wellcome Trust Human Developmental Biology Resource (HDBR) (http://www.hdbr.org/) in Hibernate-E media (ThermoFisher Scientific). All samples were obtained through elective terminations of pregnancy ...
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Immunology 2020Quote: ... Samples were thawed on ice and prepared according to the manufacturer’s instructions using a custom-made 33-plex human cytokine panel (Procartaplex ThermoFisher). In brief ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: The screening was performed with a genome-wide siRNA library (Ambion SilencerR Human Genome siRNA library v3, Thermo Fisher), composed of siRNAs targeting approximately 21000 genes arrayed on 384-well plates ...
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bioRxiv - Neuroscience 2022Quote: Human iPSCs (male WTC11 background, PMID 24509632) were cultured in StemFlex™ Basal Medium (Gibco; Cat. No. A33493-01) on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were transfected with 1.5 μg human α-synuclein-GFP plasmid DNA [48] using lipofectamine-LTX (Thermofisher) on DIV6 and imaged on DIV10 using an inverted ZEISS Z1 Cell Observer fitted with a Colibri2 cool LED 5-channel fast wavelength switching system ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA expression analysis was performed with Affymetrix Human ClariomS® microarray using the WT Plus Reagent kit (ThermoFisher Scientific). As starting material ...
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bioRxiv - Immunology 2021Quote: ... The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ) (CaptureSelect; Thermo Fisher), and incubated 20min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-CMAH cells were prepared by transfection of the human CMAH gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Clones with Neu5Gc expression were determined by HPLC analysis as previously described (63) ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... Human WT (GM03652), NPC1 (GM03123), and NPC2 (GM18455) fibroblasts (Coriell Institute, Camden, NJ) were maintained in DMEM media (Invitrogen) supplemented with 15% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... and C were selected and designed using MEGA6 software [30] and evaluated by the Thermofisher custom genomic TaqMan assay and design pipeline for non-human templates (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping) and analyzed using the ABIprism 7900HT (Applied Biosystems): SpaP SNP2137-2139 [GT/CG] ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).