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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Genes encoding full-length human TAK1 and Mfn2 were fused with a 5’ hexahistidine-tag by PCR and then cloned into pFastBac1 vector (Life Technologies). Once constructed ...
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bioRxiv - Bioengineering 2022Quote: ... a human keratinocyte cell line featuring constitutive expression of human telomerase reverse transcriptase (hTERT) were cultured in FAD medium (3:1 DMEM : Ham’s F12 Medium (ThermoFisher®, 11320074), supplemented with 1% PS ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Microbiology 2022Quote: J774.1 mouse macrophages (ATCC) or primary neonatal normal human dermal fibroblasts (Neo-NHDF) (ATCC) were cultured in 10 mL DMEM (Gibco, ThermoFisher) in T75 flasks (VWR ...
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bioRxiv - Microbiology 2022Quote: ... Fibrinogen aggregates were stimulated using 0.2mg/mL of purified fibrinogen from human plasma with an Alexa Fluor 488 conjugate (Invitrogen, Waltham, Massachusetts). Images were collected using an Olympus FluoView FV10i Confocal Laser Scanning Microscope under 60x magnification with an additional 2x zoom ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/4500) (all from Jackson Immunosearch Laboratories, Inc.) or goat anti-human IgG (γ-chain specific) HRP conjugate (1/50 000) (Invitrogen) were added and samples were incubated at 20-24°C for 60 minutes ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in a confluent monolayer of human foreskin fibroblasts (HFFs) maintained in Dulbecco’s Modified Eagle medium GlutaMAX™(DMEM+ GlutaMAX™, Gibco) supplemented with 10% foetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Quantitation of mitochondrial size in mouse and human MSCs were done by staining with Mito-tracker Red (Invitrogen/Thermo Fisher Scientific) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Quantitation of mitochondrial size in mouse and human MSCs were done by staining with Mito-tracker Red (Invitrogen/Thermo Fisher Scientific) using manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 TM were resuspended in 1 mL RPMI media and either left unstimulated or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2022Quote: ... An inoculum of 2.5×104 CFUs of bacteria in 25 uL was mixed with 75 μL human serum from healthy volunteers (Cat# BP2657100; Fisher Scientific). The mixture was incubated at 37 °C and aliquots were taken at baseline and 1h to calculate viable CFUs ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat antibody conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/500 in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermofisher Scientific). The amplicons were quantified using Agilent High Sensitivity DNA kit before the samples were pooled in sets of eight ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Invitrogen #A18805), goat anti-human IgM (Invitrogen #A18835) ...
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bioRxiv - Physiology 2020Quote: ... The cells were transiently transfected for 16-24 hours with either human or mouse orthologs of TRPA1 using Fugene 6 transfection reagents and Opti-MEM (Thermofisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Microbiology 2020Quote: ... chamber slides were incubated with secondary antibody Alexa-Fluor 488 goat anti-human IgG (H+L) (Invitrogen #A-11013, Paisley, UK) diluted 1:500 and Hoechst 33342 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: DiPS 1016 SevA hiPSC line derived from human skin fibroblasts were seeded and kept in culture on Geltrex (1% Invitrogen, USA)-coated culture flasks using mTeSR (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Cell Biology 2020Quote: C2C12 myoblasts were transfected with a plasmid containing the N-terminal 88 amino acids of human cyclin B1 fused to EGFP using Lipofectamine 3000 (Invitrogen, L3000001) with the P3000 reagent according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and human CMP-N-acetylneuraminate-poly-α-2,8-sialyltransferase (ST8SIA4) (NP_005659)were codon-optimized for human expression and synthesized (Geneart, Thermo Fisher Scientific). A cDNA encoding a soluble version of human NCAM ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
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bioRxiv - Immunology 2020Quote: ... by negative selection magnetic bead sorting (Miltenyi) and activated in complete RPMI supplemented with 30 U/ml IL-2 with anti-human CD3/CD28 dynabeads (Thermo Fisher) at a 1:1 cell-to-bead ratio.
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bioRxiv - Genetics 2020Quote: ... Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies) followed by Lithium Chloride precipitation and quantitation using a NanoDrop (Thermo Fisher Scientific)
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bioRxiv - Genetics 2020Quote: ... hybridization mixtures were created from 5ul of the nick translated fluorescent RNA probe mixed with 10ul of human Cot-1 (ThermoFisher #15279011) and 2ul of Salmon Sperm DNA (ThermoFisher #15632011) ...
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bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Plates were slapped dry and washed 4x with TBST using a BioTek plate washer followed by addition of Invitrogen anti-Human IgG (ThermoFisher A18817) and one hour incubation at 37C ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Cell Biology 2021Quote: WT or ATG13-/- MCF10A cells (human breast epithelial), expressing GFP-LC3A (human), were prepared as described previously (Jacquin et al., 2017) and cultured in DMEM/F12 (Gibco, 11320074) containing 5% horse serum (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... before incubation with the DAPI and secondary goat anti-human IgG fluorescently labeled with Alexa Fluor 488 (A-11013, ThermoFisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Wells were then washed four times in 1xDPBS followed by incubation with goat anti-human IgG Alexa Fluor 594 (Thermo Fisher) at a dilution of 1:200 in 1xDPBS for 30 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... human hepatocellular carcinoma) and HRT-18G (ATCC CRL-11663; human colorectal adenocarcinoma) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco Life Technologies) supplemented with 8% heat-inactivated fetal bovine serum (HyClone ...
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bioRxiv - Microbiology 2020Quote: ... murine alveolar macrophages MH-S (ATCC CRL-2019) and human monocytes THP-1 (ATCC TIB-202) were grown in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco 21875) supplemented with 10% heat-inactivated fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and ME49 hxgprt-ko ku80-ko (ME49) were grown in confluent human foreskin fibroblasts (HFF, ATCC SCRC-1041) in DMEM (Gibco 41965039) supplemented with 5% fetal calf serum and 40 μg/ml gentamycin (Gibco 15710-049) ...
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bioRxiv - Immunology 2020Quote: Human iPSC-derived microglia (Tempo-iMG™; Tempo Bioscience, CA) was cultured in DMEM/F-12 (Thermo Fisher, Waltham, MA, USA) supplemented with 1X N2 supplement ...