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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
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bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
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bioRxiv - Cell Biology 2024Quote: ... the human embryonic kidney cell line HEK293T (purchased from ATCC) and the human hepatocellular carcinoma HepG2 (purchased from ATCC) were maintained in DMEM medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... The iPSCs were resuspended in human embryonic stem media with 10 ng/mL of bFGF (beta fibroblast growth factor, Invitrogen) (HES-10 ...
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bioRxiv - Microbiology 2020Quote: 293T cells (ATCC) and 293FT cells (Invitrogen) were cultured in Dulbecco’s modified Eagle media (DMEM ...
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bioRxiv - Cell Biology 2022Quote: Mother cells were stained using CFSE (Cell Trace CFSE Cell Proliferation kit Thermofisher), 5×105 cells were placed in a 60mm plate in 5mL of culture medium mixed with 5µL of CFSE at 5 mM (final concentration 5µM ...
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bioRxiv - Immunology 2022Quote: Target cells were stained with either Cell-TraceTM CFSE Cell Proliferation Kit (Life Technologies) or CellTrace™ Violet Cell Proliferation Kit (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability and cell concentration were measured with an automated cell counter (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA-TruB1 was generated by inserting the ORF of human TruB1 with a Flag sequence into pcDNA.3.1(+) (Thermo Fisher Scientific) at the Nhe1 and EcoR1 sites ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNAs (siRNAs) specifically targeting human CD44 (siRNA IDs s2681 and s2682) were obtained from Life Technologies (Carlsbad, CA, USA). DPSCs (1×105 per well ...
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bioRxiv - Cancer Biology 2020Quote: ... were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher). Libraries were then purified via solid phase reversible immobilization (SPRI ...
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bioRxiv - Developmental Biology 2020Quote: ... Relative mRNA levels of a panel of inflammation-associated genes were initially screened using a Human Immune Taqman PCR array (4418718, ThermoFisher Scientific) and Taqman Fast Advanced Master Mix ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA samples were used for global gene expression profiling on human Clariom S Assay microarrays (Thermo Fisher Scientific, Wilmington, DE, USA), which include 24351 genes ...
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bioRxiv - Immunology 2022Quote: ... Blots were washed 3x with PBS-T and incubated for 1 h at room temperature with appropriate secondary antibodies: mouse IgGk-BP-HRP (cat# sc-516102, SantaCruz) or goat anti-human-HRP (cat# 31410, Thermo Scientific), diluted 1:10,000 in 5% blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1 ...
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bioRxiv - Neuroscience 2021Quote: ... of human iPSC-induced neurons were plated on completely dry PDL- (100 µg/ml) and laminin-(10 µg/ml; Thermo Fisher) coated 96-well or 6-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-human CD69 together with Live/Dear-near IR (1:1000 in PBS; LD-NIR; ThermoFisher Scientific; Cat no L10119) for exclusion of dead cells ...
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bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA, Thermo Fisher Scientific) sequences using RNAiMax lipofectamine transfection reagent (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... and either left them untreated (hence naive) or activated them ex vivo with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific), wherein the bead-to-cell ratio was 1:1 ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The raw spectra were searched against the Human (Homo sapiens) reference proteome database (UNIPROT) using SEQUEST HT (Proteome Discoverer v1.4, Thermo Fisher Scientific) with a Full MS precursor mass tolerance of 20 ppm and MS2 peptide fragment mass tolerance of 0.5 Da ...
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bioRxiv - Bioengineering 2020Quote: Cytokine analysis was performed on supernatants derived from cultures given the indicated treatments using a human cytokine 10-plex panel (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... human muscle biopsies from healthy donors were minced and digested in 0.8% w/v collagenase I (Life Technologies; Carlsbad, CA, USA) in DMEM ...
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bioRxiv - Neuroscience 2021Quote: The medial and baso-lateral amygdaloid nuclei were dissected from the mesial superior temporal lobe for 0.75-1 cm thick coronal slabs of frozen human brains on dry ice using a stainless steel punch (8mm diameter Thermo Fisher Scientific Integra Miltex standard biopsy punch) ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-Rabbit IgG Alexa Fluor 647 and Goat anti-Human IgG Alexa Fluor 647 were used at 1ug/ml concentration in FACS buffer (ThermoFisher Scientific). For western blotting ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral overexpression of PNUTS was achieved by cloning the full-length human cDNA of PNUTS into pLenti4/v5-DEST (Life technologies). The PP1 non-binding mutant PNUTS (W401A ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 live cells were incubated with the serum from a convalescent donor at the 1:100 dilution in PSB for 30 min followed by the incubation with secondary anti-human IgG antibodies conjugated with PE (1:250, # H10104, ThermoFisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... human female adenocarcinoma of the mammary gland) were maintained in Dulbecco’s Modified Eagle Medium High Glucose (ThermoFisher Scientific, #11-965-118), 10% foetal bovine serum (Bovogen ...
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bioRxiv - Cell Biology 2021Quote: ... Transferrin, Selenite) premix (resulting in 10 µg/mL insulin, 5 µg/mL human transferrin and 30 nM sodium selenite) (Life Technologies). One droplet (10 µL ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...