Labshake search
Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: Spectra were searched against the reviewed human database (November 2018) using Proteome Discoverer V2.2 Software (Thermo Fisher). Peptides were identified using a precursor mass tolerance of 10 ppm ...
-
bioRxiv - Molecular Biology 2022Quote: 30mg of human brain tissue was homogenized in 1ml of TRIzol (Catalog No. 15596026, Thermofisher Scientific, USA). 200µl of chloroform was added to each sample and mixed well ...
-
bioRxiv - Neuroscience 2022Quote: ... with GAPDH as housekeeping gene control (human GAPDH endogenous control, catalog#4325792, Life Technologies, Warrington, United Kingdom). The average of triplicate CT values from each sample was used to calculate the relative RNA levels (2-ΔCT) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
-
bioRxiv - Immunology 2022Quote: ... PCR products were then cloned into corresponding human IgG1 expression vectors with Gibson Assembly (Thermo Fisher Scientific) and the mixture was subsequently transformed into DH5α cells ...
-
bioRxiv - Neuroscience 2024Quote: Elastase generated granulin peptides were obtained by incubation of rPGRN with human neutrophil elastase (# RP-77526, Invitrogen). 50 ng/µl PGRN and 80 ng/µl elastase were incubated in 100 mM sodium citrate pH 6.0 overnight at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... 1:2000 dilution of secondary Alexa FLUOR-647 GOAT-Anti human IgG [H+L] (Invitrogen; #A-21445) was added to the cells for 30 minutes followed by 3 washes with FACS Buffer ...
-
bioRxiv - Systems Biology 2023Quote: ... Human iPSC spheroids were washed and growth factor-depleted using DMEM/F12 (Thermo Fisher Scientific #21331-046) for 2 h prior to stimulation ...
-
bioRxiv - Neuroscience 2023Quote: ... Secondary staining was done using a goat anti-Human Alexa Fluor (AF) 594 antibody (1:1000, Invitrogen), for 30 minutes ...
-
bioRxiv - Pathology 2024Quote: ... and CXCL1 levels were measured in the supernatant of human precision-cut liver slices using ELISA (ThermoFisher) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... Since most present-day data was generated using different SNP arrays (Axiom LAT1 and Human Origin Affymetrix), this data was phased and imputed by array using TOPMed Michigan Imputation Server ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... left in resting state or activated with Dynabeads Human T-Activator CD3/CD28 (1161D, Thermo Fisher Scientific) for 72 h ...
-
bioRxiv - Microbiology 2023Quote: ... followed by PBS washing and immunostaining with secondary AlexaFluor633 anti-human antibodies (Thermo Fisher Scientific, #A-21091) for 30 mins at 4°C in the dark ...
-
bioRxiv - Biochemistry 2024Quote: ... human full-length VPS35L (untagged, synthesized as a codon-optimized GeneString from Thermo Fisher to improve expression), VPS26C (untagged) ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). For comparing tumor cell growth in different groups ...
-
bioRxiv - Cancer Biology 2024Quote: ... and cDNA hybridized to a Mouse Clariom S Human Transcriptome Assay microarray plate (Affymetrix, Santa Clara, CA) and the plate was scanned by a GeneTitan MC Instrument (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
-
bioRxiv - Immunology 2023Quote: Immortalized human SZ95 sebocytes (Zouboulis et al., 1999) were cultured in T-75 culture flasks (Thermo Scientific) with Sebomed basal medium (DMEM/F12 ...
-
bioRxiv - Cell Biology 2023Quote: The release of CXCL10 to the culture medium was detected using Human ProcartaPlex immunoassays (Invitrogen, Vienna, Austria) following the manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: ... 8 µg of cDNA was fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix), according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... Hepatic gene expression was measured using Affymetrix Human Exon 1.0 ST v1 Array (Affymetrix, Santa Clara, CA) in adolescents and Illumina Human-6 Expression Bead Chips (Illumina ...
-
bioRxiv - Bioengineering 2022Quote: Intracellular mitochondrial presence within human heart organoids was visualized using Mitotracker Deep Red FM (Thermo Fisher Scientific). Mitotracker was prepared according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: Calcium transient activity within the human heart organoids was assessed using Fluo-4 AM (Thermo Fisher Scientific). Fluo-4 AM was solubilized in DMSO per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
-
bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
-
bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
-
bioRxiv - Biochemistry 2023Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA) ...
-
bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
-
bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
-
bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
-
bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
-
bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
-
bioRxiv - Cell Biology 2023Quote: Normal human epidermal keratinocytes (Kurabo) from Caucasian neonatal foreskin were cultured with EpiLife medium (Thermo Fisher Scientific) supplemented with 60 µM CaCl2 and HuMedia KG growth factor kit (Kurabo) ...
-
bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
-
bioRxiv - Developmental Biology 2023Quote: ... Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA ...
-
bioRxiv - Microbiology 2023Quote: ... human actin mRNA and EMCV viral RNA was performed mixing the Fast SYBR green Master Mix (ThermoFisher) with cDNA and 1µM of the forward and reverse corresponding primers ...
-
bioRxiv - Bioengineering 2023Quote: ... substrates were incubated with PBS solution containing various concentrations of human plasma fibronectin (Thermo Fisher Scientific, US) for 2 h ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) before adding the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
-
bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
-
bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... falciparum 3D737 infected erythrocytes were cultured in O positive human erythrocytes in RPMI 1640 media (Gibco, USA) containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... version 46) and humans using SEQUEST and Mascot (Version 2.4.1) via Proteome Discoverer v2.2 (Thermo Fisher Scientific). Trypsin was selected as the protease of choice with a maximum of two missed cleavages ...
-
bioRxiv - Microbiology 2023Quote: ... THP-1 human monocytes (ATCC) were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640, Gibco) supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
-
bioRxiv - Bioengineering 2023Quote: ... The tissues were then incubated with primary mouse monoclonal anti-human cardiac troponin T (Thermo Fisher Scientific) or mouse monoclonal anti-human CD3 (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using the Affymetrix GeneChip® Human Clariom S Array (Thermo Fisher Scientific), including more than 210,000 distinct probes representative of 21,448 annotated genes (Genome Reference Consortium Human Build 38 (GRCh38) ...