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Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Lungs were inflated with 1 ml of a solution containing 1 part 4% PFA/30% sucrose and 2 parts of optimal cutting temperature compound (OCT) media (Fisher Scientific). Inflated lungs were tied off and stored in 4% PFA/30% sucrose at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: C2C12 cells were plated onto 6-well plates at a 2 x105 density and 24 h later transfected with either negative control siRNA (Negative Control No.1 siRNA, Life Technologies) or custom siRNAs targeting S1P (Silencer Select siRNAs ...
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bioRxiv - Biochemistry 2022Quote: ... then placed in fresh ice-cold HBSS and cut into small pieces to be digested in prewarmed HBSS with 1 mg/ml Collagenase Type 2 (Life Technologies) at 37 °C for 5 min with gentle agitation ...
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bioRxiv - Bioengineering 2022Quote: ... and calcium-sensitive dye Rhod-2 AM (1 mg/ml dye stock solution in DMSO, Thermo Fisher Scientific, catalog number R1244). The heart was subjected to a 15 min equilibration period followed by dye staining to allow the washout of extra dye ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using conditions recommended by the supplier (1× PNK buffer without ATP, 2 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 2 h) ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... Total lipids were isolated by Folch extraction116 by adding 24 mL 2:1:0.6 HPLC chloroform (C297-4, Thermo Fisher Scientific): HPLC methanol (A454-4 ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 µL of buffer containing 20 mM of NH4Ac at 42 degrees for 2 hours followed by phosphatase treatment using 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) at 37 degrees for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with rabbit anti-goat IgG conjugated with Q-dot 655 for 2 h at 4 °C (1:350 dilution; Life Technologies). Then ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours later the slices were washed three times in PGT following by an incubation of 2 hours on room-temperature with the following secondary antibodies: anti-rabbit 488 (donkey, 1:1000, Life Technologies), anti-goat 568 (donkey ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Neuroscience 2022Quote: ... pooled from 3 pups were seeded per well on a Seahorse XF96 plate and cultured for 14 days in neuron growth medium (Neurobasal Medium, 2% B27, 0,25% Glutamax, 1% Penicillin/Streptomycin; all Thermo Fisher Scientific) with the different treatments before performing the Seahorse run.
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... After rinsing three times with TBS + 0.2% Triton sections were incubated with secondary antibodies donkey anti-rabbit Alexa Fluor 647 (1:500, Cat# A-31573, ThermoFisher, RRID:AB_2536183) and goat anti-chicken Alexa Fluor 488 (1:500 Cat# A-11039 ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten- micro-liter of the aliquot was mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher). The resulting RNA samples were fragmented ...
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bioRxiv - Microbiology 2024Quote: Phage DNA was isolated by treating 2 mL of phage solution with 1 U/μL DNase I (Invitrogen, Carlsbad, CA, USA) to remove free DNA ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated for 2 hours at RT with a mix of secondary antibodies: goat anti-chicken Alexa Fluor 647 (1:500, Invitrogen A32933) and goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane and immunoblotting was performed with the following antibodies: HA (#26183, 2-2.2.14, Invitrogen, USA, 1:1000), Myc (#2278 ...
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bioRxiv - Bioengineering 2024Quote: ... Cell cultures were then diluted 1:1000 in 1mL of 2% LB media pre-mixed with 0.1ug/mL FM1-43FX membrane stain (Life Technologies F35355). 50uL of the stained cell culture was then loaded into a transparent FEP tube and sealed for imaging.
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bioRxiv - Physiology 2023Quote: ... Samples were washed in PBST and incubated at room temperature for 2 hours with 1:200 Alexa Fluor 488- or 568-conjugated goat-species specific secondary antibodies (Molecular Probes) in blocking solution ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then incubated for 2 hours at room temperature with goat anti-rabbit Alexa 488-conjugated antibody (1:2000; A11008, Life Technologies). Sections were subsequently washed with PBS for 3 x 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with PBS and further incubated with 1:200 dilution of respective Alexa Fluor labeled F(ab’)2 fragment (Thermo Fisher Scientific Cat# A-11017 ...
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bioRxiv - Molecular Biology 2024Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of complementary DNA was performed using 1–2 μg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... the chitinous eggshells were first digested with base-bleach treatment (0.4 M KOH, 2% sodium hypochlorite [Fisher Scientific, catalog #SS290-1]) for 1.5 hours at 30°C ...
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Cell Biology 2023Quote: ... TMAs were transferred to humidity chambers and incubated for 2 hrs at RT with Alexa-Fluor-488 Wheat Germ Agglutinin (1:100; ThermoFisher, W11261) and Dapi (1µg/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... sections were incubated for 2 h with corresponding secondary antibodies: Alexa Fluor 488 goat anti-mouse IgG (1:800, A32723 Thermo Fisher), Alexa Fluor 568 goat anti-mouse IgG (1:800 ...
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bioRxiv - Immunology 2024Quote: ... followed by 2 h incubation with HRP-conjugated secondary antibody (1:10000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split when they reached 80% confluency (every 2-4 days) using 0.5 mM EDTA in 1× DPBS (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Then cells were transfected with the pre-incubated mixture of 1 µM 2-5A or 0.25 µg/ml poly I:C (Invitrogen, low molecular weight) and Lipofectamine 3000 (7.5 µl/6-well ...
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bioRxiv - Biochemistry 2023Quote: ... Two datasets were collected with a total of 5988 movies (dataset 1) or 5050 movies (dataset 2) and were recorded automatically with EPU software (Thermo Scientific) at a total electron dose of 62.2 e/Å2 (dataset 1 ...
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bioRxiv - Immunology 2023Quote: ... The heat-killed bacteria were stained 1:500 with Oregon Green 488-X succinimidyl ester (2 μL of dye from stock in 1 mL of PBS) (Thermo Fisher) fluorescent dye for 30 minutes at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... the protoplast-containing Trizol solution from PEG transformation was transferred to 2 mL Phasemaker tubes (1 mL per tube; ThermoFisher Scientific), mixed thoroughly with 300 µL chloroform ...
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bioRxiv - Genetics 2023Quote: ... The cells were then washed with DPBS before undergoing incubation with the secondary antibody (1:100 in 2% BSA, Invitrogen, 31557) at room temperature for 2 hrs in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Cell Biology 2023Quote: Whole bone marrow from 1 femur and whole periosteum from 2 femurs were isolated and digested as previously described and plated in α-MEM (Gibco) containing 20% FBS (HyClone) ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Immunology 2023Quote: ... at a 1:2 ratio with irradiated (100Gy) CEMx174 cells in R10 (RPMI 1640 medium supplemented with 10% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: DNA illustration by agarose gel electrophoresis has been conducted by exploiting 2% agarose gel and 1X TAE buffer (1 mM EDTA and 40 mM Tris-acetate, Fisher Scientific) and by applying constant voltage of 120 V for 40 to 60 min ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed twice in 1% BSA/PBS and co-stained with 0.5mL 2 μM Hoechst 33342 (Thermo Fisher, USA) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Microbiology 2022Quote: ... CXR dye and primers listed in supplementary table 2 at a final concentration of 1 μM on the StepOnePlus Real-Time PCR system from Thermo Fisher Scientific ...