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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: Peptides were desalted for 4 minutes on a trap column (1 mM ID x 2 cm, IDEx C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific). Peptides were then separated on a C18 analytical column (Waters Acquity UPLC BEH C18 Column ...
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bioRxiv - Cell Biology 2023Quote: ... The vector and insert (1:2) were ligated using T4 ligase following the manufacturer’s instructions (Thermo Fisher Scientific Cat. No. EL0011). The cloned plasmid (here after denoted as pHBx ...
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bioRxiv - Neuroscience 2023Quote: ... then the wells were filled with 1 mL Neurobasal™ Plus Medium supplemented with 2% B-27 Plus supplement and 0.5% GlutaMAX™ all from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The following day sections were washed in PBS and then incubated for 2 hours at RT in secondary antibody (Alexafluor 488 donkey anti-mouse at 1:200; Invitrogen A21202) following which they were washed ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were immersed in xylene for 1-2 minutes and secured with coverslips using Cytoseal XYL mounting media (Thermo Fisher). Mounted slides were heated on a slide warmer for 2-3 hours at 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 2 hours with secondary antibody AlexaFluor 488 Goat Anti-Rabbit IgG (1:1000 in 4% BSA, Invitrogen #A11008) at RT in the dark ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.9 and 0.2M NaCl were heated for 5 min at 95°C and then cooled to 25°C in 2°C steps with 1 min per step in a thermal cycler (Applied Biosystems). The resulting double-stranded DNA (∼1 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1 mL LB and added to a mixture of 2:1 methanol : chloroform (3.5 mL total) in borosilicate glass tubes (16×100 mm, Fisher Scientific 1495935AA). Samples were vortexed for 1 min to form a single phase ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with the following fluorophore-conjugated secondary antibodies for 2 h at room temperature: anti-rabbit Alexa Fluor 488 (1:400, goat, Invitrogen, A11008), anti-rabbit Alexa Fluor 568 (1:400 ...
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bioRxiv - Cancer Biology 2023Quote: ... F-actin was stained by incubating blocked slides for 2 hours at RT with Alexa Fluor 647 Phalloidin (1:400, Invitrogen #A22287).
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bioRxiv - Genetics 2023Quote: ... Animals were placed on a microscope slide with a 2% agar pad and immobilized with with ∼1 µl of 100 mM levamisole (ThermoFisher #AC187870100). A coverslip was applied and images for the same experiment were captured with identical exposure times.
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bioRxiv - Cell Biology 2023Quote: ... the glasses were incubated for 2 h at room temperature with secondary antibodies (1:800 Alexa Fluor 594 and 647 antibodies from Molecular Probes) and mounted in polyvinyl alcohol mounting medium with DAB-CO [52] ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 2 h at RT with AlexaFluor (AF) 488-labeled donkey anti-rabbit antibody (1:500, Invitrogen, Poland) and AF647-labeled phalloidin (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS and medium was replaced by NM+VitA (DMEM/F-12, 1% N2-supplement, 2% B27+VitA supplement (Thermo Fisher), 1% P/S) ...
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bioRxiv - Cell Biology 2023Quote: ... the plate was incubated at 65°C for 5 min and immediately chilled on ice for at least 2 min before adding 0.8 μL reverse transcription reaction mix [in 2 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064-014), 20 mM DTT (Invitrogen – 18064- 014) ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 times with PBS and incubated with the following secondary antibodies at RT for 2 h: Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; Molecular Probes) and Alexa Fluor 633-conjugated goat anti-rabbit IgG (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with secondary antibodies at room temperature for 2 h at 1:500 dilution: anti-rabbit AF594 (Invitrogen, A21207); anti-rat AF488 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 2 hours at 4°C in a 1:1000 solution of AF-568 donkey anti-mouse secondary antibody (Invitrogen #A10037) in blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM CHAPS) by centrifugation at 1000x g for 2 minutes with a micro-spin desalting column (Thermo Scientific Zeba) at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... detached cells were collected by centrifugation at 800g for 2 mins and red blood cells were removed by incubating the pellet with 1× red blood cell lysis buffer (Life Technologies) for 7 mins in RT ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 50 μL of a 1:4,000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid antibody (Invitrogen, Waltham, MA, Cat# MA536086) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015), or electroporated into Hepa1-6 cells using a Neon Transfection System 10 µl kit (ThermoFisher MPK 1096 ...
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bioRxiv - Cell Biology 2023Quote: ... 1.2 × 106 cells were transfected with 1 μg DNA or shRNA for each construct using the Neon transfection system (Life Technologies) following the manufacturer’s instructions (1200 pulse voltage ...
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bioRxiv - Bioengineering 2023Quote: ... Two sets of 100 μl of either Protein A Magnetic Beads (Thermo PI88846) (rounds 1 and 2) or Dynabeads MyOne Streptavidin T1 (ThermoFisher Scientific) (round 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). At 7 days post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of 1 mM ʟ-photo-leucine and 2 mM ʟ-photo-methionine (Thermo Fisher Scientific, Waltham, MA, USA). Incorporation of these photo-reactive amino acids (pAA ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were rinsed 2 times with PBS and incubated for 2 hours in 1:5000 Alexa Fluor 750 goat anti-rabbit secondary antibody (Life Technologies). The sections were rinsed 2 times with PBS and mounted in ProLong diamond antifade mounting media (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid) on an Acclaim PepMap100 RSLC C18 analytical column (2 μm, 75 μm id x 50 cm, [Thermo Scientific]) with a flow rate of 0.3 μl/min ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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bioRxiv - Physiology 2023Quote: ... followed by incubation for 2 h at room temperature with Alexa Fluor 680-linked anti-mouse IgG (A21058; Invitrogen, 1:5000). Signals were visualized with an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 hour probing with the appropriate secondary antibodies diluted in block: Anti-guinea pig IgG-Alexa 594 (1:250, ThermoFisher #A11076), anti-rat IgG-Alexa 488 (1:250 ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a mass ratio of 2:1 (HC:LC) using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). After transfection ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of eluted RNA was treated with 2 U of DNase using the Invitrogen Purelink DNase kit (ThermoFisher, Waltham, USA) and incubated at 37°C for 30 minutes followed by inactivation at 62°C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... repair template (Supplemental Table 1) were introduced into ARPE-19 cells using electroporation (1400 V, 20 ms, 2 pulses, Neon Transfection System, Life Technologies). After electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were resuspended in 1mL of FACS buffer (PBS with 2% FBS and 1mM EDTA) containing a live/dead dye diluted 1:2000 (Live/Dead Fixable Aqua, Invitrogen #L34957) and incubated for 10 min at RT ...
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bioRxiv - Bioengineering 2023Quote: The tissues were stained with 2 μg ml-1 CellMask™ Deep Red plasma membrane stain (ThermoFisher Scientific, Waltham, MA, USA) for at least 24 h before imaging ...
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were washed in 2 mL PBS 2x after which 200 µL of freshly made propidium iodide solution (.1% Triton-X 100 (Fisher Scientific), 50 µg/mL propidium iodide (Thermo Scientific) ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... The reaction was stopped by addition of 10 µL 10% TFA and samples were desalted by stagetipping with C18 filter plugs (Solaµ HRP 2 mg/1 ml 96 well plate, Thermo Scientific). Peptide samples were stored in 40 µL of 0.1% Formic acid at 4 °C until LC-MS analysis.
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into HEK293T cells at a mass ratio of 2:1 (HC:LC) using Lipofectamine 2000 (Thermo Fisher Scientific). On day 3 post-transfection ...