Labshake search
Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS and medium was replaced by NM+VitA (DMEM/F-12, 1% N2-supplement, 2% B27+VitA supplement (Thermo Fisher), 1% P/S) ...
-
bioRxiv - Neuroscience 2023Quote: ... The following day sections were washed in PBS and then incubated for 2 hours at RT in secondary antibody (Alexafluor 488 donkey anti-mouse at 1:200; Invitrogen A21202) following which they were washed ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
-
bioRxiv - Physiology 2023Quote: ... followed by incubation for 2 h at room temperature with Alexa Fluor 680-linked anti-mouse IgG (A21058; Invitrogen, 1:5000). Signals were visualized with an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... were grown with 3T3 feeder layer support in a 2:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM; Thermofisher; Cat # 12100061) and Ham’s F-12 nutrient mix media (Thermo Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... and the EDL was immediately incubated in plating medium (DMEM + 10% horse serum + 2% Penicillin/Streptomycin + 1% HEPES) containing type I collagenase (2.6 mg/mL; Gibco, 17100-017) in a 37°C shaking water bath for 53 minutes ...
-
bioRxiv - Bioengineering 2024Quote: ... the embryos were incubated in secondary antibody solution (1 mL CAS-Block containing 2 μL Alexa Fluor 488 (Invitrogen, A-11006), 2 μL Alexa Fluor 594 (Invitrogen ...
-
Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
-
bioRxiv - Immunology 2024Quote: ... microarray slides were blocked by incubation for 2 hours at room temperature with PBS containing 1× synthetic block (Thermo Fisher Scientific) and 0.05 % Tween 20 ...
-
bioRxiv - Microbiology 2024Quote: ... The human monocytic leukemia THP-1 cell line was thawed and cultured in RPMI media supplemented with 20% FBS and 2-mercaptoethanol (Gibco: 21985023) to a final concentration of 0.05 mM at 37°C in a humidified atmosphere at 5% CO2 ...
-
bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... PANC-1 ULK1/2 WT MEF and ULK1+ULK2 DKO MEF cells were cultured in High Glucose DMEM + GlutaMAXTM + Pyruvate (ThermoFisher Scientific), supplemented with 10% foetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... A549 and/or H1299 cells were cotransfected with 2 ug of NPRL2 plasmid DNA (pKGB2-NPRL2) and 1 ug of pcDNA3.1 vector (Invitrogen, Carlsbad, CA) containing a neomycin-resistant gene ...
-
bioRxiv - Immunology 2024Quote: ... as well as transmembrane serine protease 2 (TMPRSS2) were seeded at a density of 1 × 104 cells/well in white-walled 96-well plates (ThermoFisher Scientific) one day prior to usage (day 0) ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 15 min in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) solution (1:5000; catalog no. D1306, ThermoFisher Scientific, USA), followed by four 15-min washes in PBS with 0.1% Tween-20 ...
-
bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
-
bioRxiv - Biophysics 2024Quote: ... followed by a 2-minute incubation at 37 °C (with gentle rotation) in 1 mL warm TrypLE (Thermo Fisher Scientific, #12604013). The majority of TrypLE was removed by pipetting after incubation ...
-
bioRxiv - Cancer Biology 2024Quote: ... siRNA ID: s6696, s6697 and s6698 for HNF4A and cat# 4390843 and 4390846 for negative control #1 and #2, respectively, Thermo Fisher) and gene expression analysis was performed as described above ...
-
bioRxiv - Cancer Biology 2024Quote: ... pre-REP TIL were co-cultured with 2×107 40 Gy-irradiated allogeneic feeder PBMC (1:200 ratio) in upright 25-cm2 flask (ThermoFisher Scientific) in REP-TIL culture medium ...
-
bioRxiv - Bioengineering 2024Quote: ... For other experiments, 2 ESC cell lines (H1 and H9, WiCell #WA01 and #WA09 respectively) and 1 episomal iPSC cell line (ThermoFisher, #A18945) were used ...
-
bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
-
bioRxiv - Cell Biology 2024Quote: ... Click-it reaction was performed after staining with primary antibodies by incubating cells in Click-it buffer (100 mM Tris-HCl pH 8, 2 mM CuSO4, 1 ng Alexa Fluor 647 Azide (Life Technologies), and 100 mM ascorbic acid for 30 min at room temperature (RT) ...
-
bioRxiv - Biochemistry 2024Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol for a 25 mL culture ...
-
bioRxiv - Physiology 2024Quote: Isolation of ADSC-derived small extracellular vesicles (sEVs) involved culturing ADSCs in high-glucose DMEM with 2% exosome-depleted FBS and 1% penicillin/streptomycin (Gibco, A2720803) for 48 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times for 15 min in PBS before a 2 h incubation with either a donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (dilution 1:500, Invitrogen, #A21208) or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500 ...
-
bioRxiv - Microbiology 2024Quote: MDCK or MDCK SIAT1 cells were seeded in 96-well plates at 1-2 ξ 104 cells/well in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
-
bioRxiv - Neuroscience 2024Quote: ... were transfected with Glod4-targeting siRNAs (siRNA Glod4 #1: Cat. #43390816 ID s84641; siRNA Glod4 #2: Cat. #4390816 ID s84642, Thermo Scientific); siRNA scrambled (siRNAscr ...
-
bioRxiv - Neuroscience 2024Quote: ... Wells were washed with wash buffer (0.05% Tween 20 in PBS) and blocked with 2% BSA (Biowest) in PBS containing 1% Casein blocker (Thermo Fisher Scientific) for 1 h at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... and rinsed and then incubated for 2 h with appropriate Alexa Fluor-conjugated secondary antibodies (R37117, Thermofisher, Waltham, MA, 1 : 500). Fluorescent images were acquired using a Nikon A1R+ laser scanning confocal microscopy system on a Nikon Ti2-E inverted microscope ...
-
bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... The epithelial solution was spun down at 1000 g for 2 minutes and then resuspended in 1 mL of TrypLE express enzyme (ThermoFisher #12604013) for 5 minutes at 37°C ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... samples were incubated in Tyramide development solution (2% Dextran sulfate, 0.0015% hydrogen peroxide, 0.2mg/ml Iodophenol, 1:100 Alexa Fluor 594 Tyramide Reagent (Thermo Scientific, Cat. # B40957) in PTw ...
-
bioRxiv - Molecular Biology 2024Quote: ... and their bladders were excised and placed into 2 mL conical-bottom screw-cap tubes (USA Scientific #1420-8700) on ice containing 1 mL TRIzol (Invitrogen #15596018) and six 2.8 mm diameter ceramic beads (Omni International #19-646) ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmids were co-transfected at a 1:2 heavy to light chain ratio into Expi293F cells using the Expifectamine 293 Expression Kit (Thermo Fisher), and antibodies were purified with protein A agarose (Invitrogen) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cells were maintained in 100 mm culture dishes with media changes every other day and passaged 1:2 two to three times per week using trypsin-EDTA (0.25%, ThermoFisher #25200-072). After six weeks of culturing ...
-
bioRxiv - Neuroscience 2024Quote: ... and donkey anti-mouse antibody conjugated with Alexa Fluor 488 (catalog# A-212-2, RRID AB_141607, Thermo Fisher Scientific 1:500). To assess co-expression of ChAT and Chrimson ...
-
bioRxiv - Neuroscience 2024Quote: Proteins were extracted in lysis buffer (10 mM Tris-HCl, 2 mM EDTA, 1% SDS) containing protease and phosphatase inhibitor (1862209 and 1862495, Thermo Scientific). To measure protein concentration ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 2 mM MgCl2 supplemented with 2 μM Ultrapure Bovine Serum Albumin (Invitrogen) for 10 min at 25 °C ...
-
bioRxiv - Immunology 2021Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
-
bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37 °C with 2 ul of FastDigest Mph1103I (ThermoFisher cat. no. FD0734) and 2 ul of FastDigest Eco105I (ThermoFisher cat ...
-
bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37°C with 2 ul of FastDigest FspAI (ThermoFisher cat. no. FD1664) and 2 ul of FastDigest PmeI (ThermoFisher cat ...
-
bioRxiv - Immunology 2019Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
-
bioRxiv - Cell Biology 2019Quote: ... goat anti-mouse Alexa Fluor 488 F(ab’)2 Fragment (2 μg/ml, A11017; Molecular Probes), Alexa Fluor 568–phalloidin (1:200 ...
-
bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
-
bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37 °C with 2 ul of FastDigest BamHI (ThermoFisher cat. no. FD0054) and 2 ul of FastDigest SbfI (ThermoFisher cat ...
-
bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
-
bioRxiv - Immunology 2019Quote: ... Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2020Quote: ... IL-2 was measured from culture supernatants using a mouse IL-2 ELISA kit (Thermo Scientific).
-
bioRxiv - Cell Biology 2019Quote: ... along with 2 ml of phenol red-free Neurobasal media supplemented with 2% B27 (17504044, ThermoFisher) and 30 mM HEPES (H0887 ...
-
bioRxiv - Genetics 2020Quote: 10T1/2: C3H10T1/2 cells were grown using the following media recipe: DMEM (ThermoFisher Scientific, 11965118), 10% FBS (ThermoFisher Scientific 26140079) ...