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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed on a QuantStudio 6 using SYBR Green qPCR Master Mix (ThermoFisher Scientific) and primers specific to each target gene and Ubc as a control ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was run on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems) with iTaq Universal SYBR Green Supermix (BioRad 1725121 ...
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bioRxiv - Cancer Biology 2023Quote: AML cells (6 x104 cells/cm2) were seeded for 24h on poly-D-Lysine (Gibco) coated Lab-Tek II Chamber Slides (Nunc ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 3 mins at 90C and separated on a 6% denaturing PAGE gel (Invitrogen). The RNA was then transferred to a HyBond-N+ nylon membrane (GE Life Sciences ...
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bioRxiv - Bioengineering 2023Quote: ... electroporated hepatocytes were maintained in HMX media in 6-well Collagen I-coated plates (Gibco) and cultured at 37°C in a humidified incubator with 5% CO2 and ambient oxygen levels ...
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bioRxiv - Bioengineering 2023Quote: ... ODCs were first generated by dissociating Day 6-8 PPTO.46 PDOs with TrypLE (Gibco) dissociation solution for 30-40 minutes in an incubator ...
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bioRxiv - Microbiology 2023Quote: ... The AePer50 qPCR was performed on a QuantStudio 6 Real-Time PCR System (Applied Biosystems), with cycling conditions as follows ...
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bioRxiv - Bioengineering 2023Quote: ... for PEG-RGDS and 6-8 kDa MWCO dialysis membrane for PEG-PQ-PEG (Thermofisher), then lyophilized ...
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bioRxiv - Neuroscience 2023Quote: ... inside 6-well plates with culture medium containing 78% Neurobasal-A without phenol red (Invitrogen), 20% heat-inactivated equine serum (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was performed on a QuantStudio 6 Real-Time PCR System (Thermo Fisher). Primers used in Real time-qPCR experiments are listed in Supplementary Table S2 ...
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bioRxiv - Physiology 2023Quote: ... The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies), using TaqMan™ Universal Master Mix II ...
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bioRxiv - Cell Biology 2023Quote: ... 6 × 105 cells/ml were plated in a 90 mm petri dish (101R20, Thermo Scientific). Cells were treated with LLOMe for 30 minutes at a concentration of 1 mM by dilution in RPMI medium ...
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bioRxiv - Neuroscience 2023Quote: ... The reaction was performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) or the StepOne Plus Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: The cell wall stressor assays were done using 6-well plates (Invitrogen, Waltham, MA, USA). The wells were filled with GMM or SMM supplemented with increasing concentrations (20 ...
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bioRxiv - Molecular Biology 2024Quote: ... The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies), using TaqMan™ Universal Master Mix II ...
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bioRxiv - Neuroscience 2024Quote: ... Bound cytokine was detected using a biotinylated IL-6 detection antibody (ThermoFisher Scientific, MP5-32C11), streptavidin horseradish peroxidase (strep-HRP) ...
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bioRxiv - Cell Biology 2024Quote: 4-6×107 mESCs were cross-linked by incubating them in DPBS (Gibco, Cat #14190094) supplemented with 1% formaldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was flowed over 6 ml of washed Ni-NTA resin (ThermoFisher Scientific) in a gravity column ...
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bioRxiv - Neuroscience 2024Quote: SH-SY5Y cells were plated (100,000 cells/well) on 6-well plates (ThermoFisher Scientific, 353046) and allowed to adhere for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... on 6-well plates coated with Gibco Geltrex hESC-qualified Basement Memebrane (ThermoFisher cat #A1413301) using previously established maintenance protocols97 ...
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bioRxiv - Cell Biology 2024Quote: ... For qPCR, purified RNA was reverse transcribed (Thermo, 4368814) and quantified (Applied Biosystems, Quantstudio 6) with SYBR green reagent (Thermo ...
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bioRxiv - Biochemistry 2024Quote: ... then harvested by centrifuging at 4,000 ×g (Fiberlite F9-6×1000 LEX rotor, Thermo Scientific) for 10 min and resuspended with HBS buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions and run on a QuantStudio 6 instrument (Thermo Fisher Scientific). Serial dilutions of RNA standards with known copy numbers were run in parallel in each run to calculate RNA copy numbers in the standard.
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bioRxiv - Biochemistry 2024Quote: ... then harvested by centrifuging at 4,000 ×g (Fiberlite F9-6×1000 LEX rotor, Thermo Scientific) for 10 min and resuspended with HBS buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies), using TaqMan™ Universal Master Mix II (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate, ThermoFisher Scientific) with a 6 mm diameter dropper ...
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bioRxiv - Biophysics 2021Quote: 106 T-cells were incubated in T-cell media supplemented with 5 µg ml-1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 2 h at room temperature with the following primary antibodies: rabbit anti-EEA1 (1:3000, Thermo Fisher Scientific Cat# PA1-063A ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... and then incubated overnight at 4°C in 2 ml TBST/5% milk with a 1:1,000 dilution of anti-CPY Monoclonal antibody 10A5B5 (Invitrogen, A-6428). The nitrocellulose was then washed 3 times in 20 ml TBST at room temperature for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... The Halo-H2A-expressing U2OS cell line was engineered to stably express NES-mCherry-LINuS (import probe) or NLS-mCherry-LEXY (export probe) by retroviral transfection and selection with 2 μg ml−1 blasticidin (#A1113903, Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: Cells were seeded at for screening at 1×105 cells per well of a 2 well Lab-Tek chamber slide (Thermofisher, 155360). The on-the-fly real-time capture was done using the 488 nm laser channel for excitation and using the 520 nm emission detector to collect the GFP signal and the 647 nm excitation laser and 667 nm emission detector for the segmentation channel ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty million cells were diluted in 2 mL of GBSS containing 1% FBS and stained with 5 μg/mL of Hoechst 33342 (Invitrogen H3570) for 1 h at 32 °C ...
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bioRxiv - Physiology 2020Quote: ... frozen left-ventricular tissue was placed in Isolation solution (Supplemental Table 2) containing protease and phosphatase inhibitors (1:100 ratio, Fisher Scientific), 10 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were passed through an immobilized pepsin column (2 mm□× 2 cm) at 120 μL min−1 and the digested peptides were captured on a C18 PepMap300 trap column (Thermo Fisher) and desalted ...
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bioRxiv - Biochemistry 2021Quote: ... blotted for 1–2 s and plunge frozen into a liquid ethane/propane mixture with a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were incubated for one hour with the secondary fluorescent marker-conjugated antibodies at room temperature and counterstained with 1 μg/mL of 4’6-diamidino-2-phenylindole (DAPI, Molecular Probes, USA). Images were collected using the EVOS microscope ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and oligonucleotide primers (Eurofins Genomics, Louisville, KY; Tables 1 and 2) on a 7500 Real-Time PCR system (Thermo Fisher Scientific). For X ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and then incubated for 2 hours at room temperature with biotinylated secondary antibodies (anti-rabbit 1:1000; Thermo Scientific) in the same buffer solution ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in PBT-X solution (2% NHS, 0.2% TritonX-100 in PB) with primary antibody (1:1000 polyclonal rabbit anti-GFP, ThermoFisher Scientific, #A11122) at room temperature for 24hr ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was washed with PB and incubated overnight in PBT-X (2% NHS, 0.2% TritonX-100 in PB) with secondary antibody (1:1000 AlexaFluor 488-conjugate anti-rabbit, ThermoFisher Scientific, # A27034). Tissue was washed with PB and mounted onto gelatinised slides ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in Alexa Fluor 488 donkey-anti-mouse (1:500 in TNB, Invitrogen, RRID:AB_141607). Following three washes with TNT ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% Triton X-100 in PBS) for 2 h at room temperature and then with primary antibody (rabbit anti-GFP, 1:1000, Invitrogen, G10362) diluted in blocking buffer overnight at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 hr incubation at RT in secondary antibody diluted 1:500 in block (goat anti-rabbit Alexa 568, Life Technologies; 1:500 goat anti-chicken Alexa 488 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in PBT-X solution (2% NHS, 0.2% TritonX-100 in PB) with primary antibody (1:1000 polyclonal rabbit anti-GFP, ThermoFisher Scientific, #A11122) at room temperature for 24hr ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a 2 ml deep-well plate (1 ml of suspension per well, eight replicates per treatment) and a honeycomb plate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Genomics 2019Quote: ... All six pX300-gRNA plasmids were pooled and 1 µg was transfected into 2 million E14tg2a cells using Lipofectamine (Life Technologies). A separate pX300 empty vector was also transfected into E14tg2a to serve as a negative control ...