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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... incubation with secondary antibody was performed in 0.5% BSA/PBS for 1 hour at room temperature and nuclei were stained with 2 μg/ml Hoechst 33342 (Life Technologies). Images were acquired using the Leica SP8 confocal or Leica DMI6000-SD microscopes ...
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bioRxiv - Biophysics 2020Quote: ... At the end the cells were resuspended in 2 ml of medium (DMEM GlutaMax supplemented by 10 % FBS and 1 % antibody from Thermo Fisher Scientific, Invitrogen), counted via the trypan blue method and put in T-75 flask with approximately 20 ml of medium ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the indicated primary antibodies in PBS 2% FBS buffer overnight following by 45 min incubation with secondary antibodies and 1 μg/ml Hoechst (ThermoFisher, H1399).
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated 2 hours at room temperature with a secondary antibody donkey anti-rabbit Alexa 555 (1:500 in PBST, ThermoFisher, A31570). After Hoechst counterstaining ...
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bioRxiv - Cell Biology 2022Quote: The fractionated peptides were resuspended in 1% (v/v) TFA + 2% ACN and roughly 1.5 μg were injected onto nanoHPLC Dionex UltiMate 3000RS (Thermo Fisher Scientific) in combination with Thermo Orbitrap Fusion ...
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bioRxiv - Cell Biology 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit, Invitrogen, 32260; anti- mouse, Invitrogen, 32230). Coverslips were washed 3 times with blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 1×106 Cmtr1KD cells were transfected with 2 μg pPyPCAGGS vector (empty-vector or HA-CMTR1) using Lipofectamine 2000 (Life Technologies). Successfully transfected cells were selected with puromycin (1 μg/mL).
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was stained in 20 ml of 1 x TBE buffer containing 2 μl of SYBR Gold stain (ThermoFisher Scientific) for 5 minutes at room temperature and a band migrating just above the 35 nt oligo was cut from the Ribo-seq sample lane.
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 μm ID x 15 cm, 2 μm, Thermo Scientific) mounted on a DPV ion source (New Objective ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 (15 min) and stained for 30 min with 2 μg/ml HCS Cell Mask stain (Life Technologies). Syncytia were counted microscopically in triplicate wells ...
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bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated at 37 °C and images were taken manually every 1 h for 24 h using Evos FL Auto 2 inverted microscopy (Thermo Scientific).
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: ... cells were incubated with 20-50 nM SiR-tubulin dye (SpiroChrome) for 1-2 hours and transferred to L-15 medium (ThermoFisher Scientific) before imaging ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated at room temperature for 2 hours along with Alexa Fluor 488 phalloidin (Invitrogen #A12379, 1:40 dilution). Secondary antibodies were removed ...
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bioRxiv - Molecular Biology 2022Quote: ... DHIV3-GFP and env-expression plasmids were co-transfected at a mass ratio of 2:1 using lipofectamine 2000 (ThermoFisher, 11668030). shRNA encoding lentiviral particles were produced by co-transfecting pHIV7 ...
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bioRxiv - Plant Biology 2022Quote: ... was used for floral dip transformation of pTAN1:CFP-TAN1 and selected on 1/2 MS plates containing 100 μg/mL gentamicin (Fisher Scientific). T1 seedlings were screened for mScarlet and CFP signal and then transferred to soil to self.
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Cancer Biology 2022Quote: ... confluent stromal cells were stained for 10 minutes with 2 μM MitoTracker Green FM and 1 μM MitoTracker DeepRed FM (Molecular Probes), washed twice ...
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bioRxiv - Cell Biology 2022Quote: Wild-type HeLa cells (HPA/ECACC 93021013) or FCHO1/2 KO HeLa #64/1.E (Umasankar et al., 2014) were cultured in DMEM with GlutaMAX (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Developmental Studies Hybridoma Bank) and secondary antibody goat α-mouse F(ab’)2 fragments labelled with Alexa Fluor647 (1:500, A21237, Thermofisher). Boutons were visualized using Alexa Fluor488 conjugated goat α-horseradish-peroxidase antibody (α-hrp ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 2 hours at room temperature protected from light in fluorescently conjugated Alexa Fluor secondary antibodies 1:500 (ThermoFisher Scientific) and DAPI (D3571 ...
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bioRxiv - Neuroscience 2021Quote: ... and rinsed and then incubated for 2 h with appropriate Alexa Fluor-conjugated secondary antibodies (R37117, Thermofisher, Waltham, MA, 1 : 500). Fluorescent images were acquired using Olympus IX81 or Zeiss 880LSM microscopes.
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bioRxiv - Neuroscience 2021Quote: ... in 2% NHS / 0.4% Triton™ X-100 in PBS with primary antibodies against Pax2 (1:500, rabbit, Invitrogen 71-6000), Calbindin D28-K (1:10.000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfection solution was prepared by adding 1 μg/ml purified DNA and 2 μg/ml linear PEI in Serum- Free Hybridoma Media (Gibco®) supplemented with 1 % FCS ...
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bioRxiv - Immunology 2021Quote: ... and then 1-2 μg of total RNA was used to prepare cDNA using high-capacity cDNA Reverse Transcriptase kit (Applied Biosystems). Quantitative PCR was performed using SYBR Green master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... and incubation at 37°C for 1 hr and 2) 1 hour incubation at 50°C following addition of 15μl Proteinase K (20 mg/ml, Thermo Scientific™) and 30μl Proteinase K digestion buffer (0.1M NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Micropost arrays were passivated using a 1:2 mix (0.5 mg/ml in PBS) of fluorescently labeled (DyLight 405 NHS ester; Fisher Scientific Ireland) endotoxin-free bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: Cells were set at a concentration of 1 x105 cells/mL 24 hrs prior to EdU addition (5-ethynl-2’-deoxyuridine; Life Technology, Thermo Scientific) in media free from thymidine prepared using Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed in 0.1 M TB (2 ×5 minutes) and counterstained with Hoechst 33342 (1:20000, #62249, Thermo Fisher Scientific) diluted in 0.1 M TB ...
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bioRxiv - Microbiology 2021Quote: ... The liquid portion of the sample was removed and diluted 1:2 by volume in sterile PBS without calcium or magnesium (Gibco, 10010023). The sample was spun again at 3000 rpm for 5 minutes at RT ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells stably expressing TfR-GFP were plated on fibronectin coated MatTek dishes and the next day incubated with 1 μM Alexa 647 labelled AP3 aptamer in blocking buffer (2 mM salmon sperm DNA, ThermoFisher #15632011), 2 mM yeast tRNA (ThermoFisher #AM7119 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed three times in 0.1 M PBS and incubated for 2 h at room temperature in donkey anti-goat AlexaFluor546 (1:500, Invitrogen, #A-11056), donkey anti-goat FITC (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours with the secondary antibody (Alexa fluor™ 488 goat anti-rabbit 1:1000, Invitrogen), mounted on slides and embedded in fluoromount.
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with wash buffer (DPBS containing 1% FBS) and incubated with 2 µg/ml of streptavidin-PE-Cy7 conjugate (Life Technologies) on ice for 30 min and then washed with wash buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Captured cells were stained with 2 μM green-fluorescent calcein-AM and 4 μM red-fluorescent ethidium homodimer-1 (Molecular Probes) and visualized under an EVOS FL cell imaging station (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2 h at RT with an Alexa Fluor 555 nm-conjugated goat anti-mouse secondary antibody (1:500; Molecular Probes, ThermoFisher Scientific cat#A21127 ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Immunology 2022Quote: ... The three Fabs were expressed by co-transfection of plasmids of heavy and light chain at the ratio of 2:1 in ExpiCHO expression system (Life Technologies) for two weeks according to the manufacturer’s manual ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Genomics 2019Quote: Single cells were stained with 4 μM Ethidium homodimer-1 and 2 μM Calcium AM (ThermoFisher LIVE/DEAD Viability Kit L3224) in the dark at room temperature for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... permeabilized with 0.1% Triton X-100 (15 min) and stained for 30 min with 2 µg/ml HCS Cell Mask stain (Life Technologies). The plates were imaged using the CellInsight™ CX5 High Content Imaging Platform (Thermo Scientific) ...