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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Next day the cells were washed twice with PBS in RT and incubated with HSV 1/2 polyclonal antibody FITC conjugate (ThermoFisher Scientific) at a dilution of 1:100 in PBS for 3hrs at RT and then washed twice in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... De novo synthesis of Sgo1 protein was quenched by the addition of 2% glucose and 1 mg/mL cycloheximide (Acros Organics).
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bioRxiv - Cell Biology 2019Quote: ... Cells were sorted in the following lysis buffer: 2 μl TE buffer pH 8.0 with the following components: 1% NP-40 (Thermo Fisher, 28324), 0.1% Triton X-100 (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... mid-L3 larvae were fed on fly food containing 1 mM 5-ethynyl-2 deoxyuridine (Click-iT EdU, Thermo Fisher Scientific) for 4 hours for hemocyte staining and 3 hours for lymph gland staining at 25°C ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were incubated for 2 hours in a solution with the secondary antibody (Alexa 594, anti-mouse, 1:1000, Thermo Fisher) at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed ten times with PBS for 2 min and next incubated with 1:150 anti-rabbit goat monoclonal antibody conjugated to 15 nm gold particles (Fisher Scientific) in PBS at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transfer to a new tube ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 1xPBS supplemented with 2 % fetal bovine serum (FBS, Hybclone) and 1 μg/mL 7-AAD (Life Technologies), filtered through a 40 μm strainer ...
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bioRxiv - Immunology 2020Quote: ... in PBS/0.5% BSA at room temperature for 2 h and washed in PBS/0.2% Tween-20 at room temperature for 1 h five times before mounting with SlowFade Diamond Antifade Mountant (S36963, Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Biophysics 2020Quote: ... 1 μL of 20 mM latrunculin A or 1 μL of DMSO was mixed with 100 μL of YE5S medium containing 2% (w/v) agarose UltraPure agarose (Invitrogen Corporation) kept in a water bath at ~70 °C ...
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bioRxiv - Microbiology 2021Quote: For GFP RIPs HEK-293Ts were transfected with 2μg GFP or GFP-ORF57 plasmids with DNA in a 1:2 ratio with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further washed three times with washing buffer (0.02-0.01% Triton X-100 + 0.3% BSA in PBS) and incubated with 2° Ab at 1:1000 dilution (Goat Ab-AF 594, Life Technologies, A11005) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with TBST and incubated in secondary antibody (2% NGS in TBST) with Alexa 568-conjugated goat anti-rabbit (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were washed the following day with TBS and incubated in secondary antibody solution (2% NGS in TBST) with goat anti-mouse 488 (1:5000, Thermo Fisher Scientific Cat# A-11001 ...
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bioRxiv - Microbiology 2020Quote: ... ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 1 ml TRIzol Reagent (Thermo Scientific) using a Tissue Lyser II (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and beads were washed 5x 2 minutes in 1 mL of ChIRP Wash Buffer (2x NaCl-Sodium Citrate (SSC, ThermoFisher Scientific), 0.5% SDS ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA samples were prepared from freeze-dried cells (CHRIST Alpha 1‐2 LDplus lyophilizer, Osterode, Germany) derived from untreated and farnesol-treated cultures using TRISOL (Invitrogen, Austria) reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 555 donkey anti-mouse (1:1000, Life Technologies-Invitrogen). Cells were finally stained with DAPI (1:5000 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... in a RNase/DNA-free fume hood 2 µl of RNA was mixed with 1 µl of dNTP mix (Thermo Scientific), 1 µl of Oligo-dT primer (Merck ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Cell Biology 2020Quote: ... and either pINDUCER-20-IRS2 or pHAGE-FLAG-HA-NTAP-IRS2 in a 4:2:1 DNA ratio using Lipofectamine 3000 (Invitrogen, L3000001) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... with 10% FBS (Seradigm) or 15% FBS for Mfn1/2-null mouse embryonic fibroblasts and 1% penicillin/streptomycin (Thermo Fisher Scientific). Mouse embryonic fibroblasts cells (Mfn wild type ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were resuspended in buffer (PBS/2% FCS/ 1% penicillin/streptomycin) containing a viability dye from the Sytox series (Life Technologies) or DAPI (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 hours at RT with appropriate secondary antibodies: goat anti-rabbit Alexa Fluor 633 (1:500, Invitrogen, A21070), and goat anti-mouse Alexa Fluor488 (Jackson Immunoresearch ...
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bioRxiv - Biochemistry 2021Quote: S9C:WT (1:2) αSyn amyloid fibres were incubated with Alexa Fluor 647 maleimide dye (10-fold molar excess, Thermo Fisher Scientific) and Biotin-X ...
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bioRxiv - Microbiology 2021Quote: ... 11 cDNA fragments with 70 bp end-terminal overlaps which spanned the entire SARS-CoV-2 isolate Wuhan-Hu-1 genome (GenBank accession: NC_045512) were produced by GeneArt™ synthesis (Invitrogen™, ThermoFisher) as inserts in sequence verified ...
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bioRxiv - Immunology 2020Quote: ... SARS-COV-2 Spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg SARS-CoV-2 S-2P or RBD) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
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Metal transporter SLC39A14/ZIP14 modulates regulation between the gut microbiome and host metabolismbioRxiv - Physiology 2021Quote: ... the pellets were resuspended in lysis buffer (Tris-HCl, 137mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA) with protease and phosphatase inhibitors (Thermo Scientific) added along with the PMSF (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were rinsed three times in PBS and incubated with F(Ab’)2-goat anti-mouse IgG-Alexa 594 (1:500, #A11020, ThermoFisher Scientific) and DAPI (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were incubated for 2 hours at room temperature (RT) in carrier solution containing Alexa-488 conjugated secondary antibodies (1:200, Life Technologies). For phalloidin and DNase I treatment ...
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bioRxiv - Evolutionary Biology 2021Quote: ... (diluted 1:400 in 2% BSA) and a second step of 1 h of incubation with an Alexa Fluor 488 conjugate secondary antibody (Molecular Probes) (diluted 1:1000 in 2% BSA) ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Immunology 2021Quote: ... OHSCs were washed and incubated with Alexa Fluor™ 488 donkey anti-rabbit IgG (2 μg ml−1; Invitrogen, A-21206) secondary antibody for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibody was F(ab’)2-Goat anti-Rabbit IgG conjugated with Alexa Fluor 350 dye (Invitrogen, 1:500 dilution).
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bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...
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bioRxiv - Cancer Biology 2021Quote: ... an additional 100 μL of EGM-2 without growth factor was added with 1.5 μL of 1 mg/mL calcein-am (ThermoFisher Cat # C1430). Cells were then imaged 30 minutes later at the 4-hour timepoint ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... samples were homogenized and ∼15g was diluted 1:2 (w/v) in cold phosphate-buffered saline (PBS; Gibco, ref.:10010-031), vortexed for 1 minute and centrifuged at 4000xg for 15 minutes at 4°C instead of 3000xg ...
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bioRxiv - Developmental Biology 2022Quote: Animals injected with either shRNA #1 or #2 were collected at 48hpf and lysed in 500 µl of TRIzolTM reagent (Invitrogen, 15596026) by vortexing extensively ...
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bioRxiv - Cell Biology 2022Quote: ... islets from Pdx1-CreERTg/0 Rosa26mTmG/- transgenic mice were washed 3× in room temperature PBS + 2 mM EDTA and then dispersed by constant gentle pipetting in 1 mL of 0.025% Trypsin-EDTA (Thermo Fisher Scientific) in PBS at room temperature for 8-10 minutes ...