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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed with TBST and incubated for 1 h in TBST/2% BSA with secondary antibody (Immunopure goat anti-mouse # 31430, Thermo Scientific, and peroxidase-labeled affinity anti-rabbit # 04-15-06 ...
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...
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bioRxiv - Neuroscience 2019Quote: ... cells were dissociated with TrypLE™ and replated in StemFlex with RevitaCell and 2 μg/mL Doxycycline (Fisher Scientific, BP2653-1) to induce TetO gene expression ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed and incubated at room temperature for 2 h in donkey anti-mouse alexa fluor 594 (1:1000, Life Technologies) and donkey anti-rabbit alexa fluor 488 (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely recon-stituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Physiology 2020Quote: ... cDNA (1 μl) was added to a reaction master mix (10 μl) containing 2× SYBR green qPCR reaction mix (Thermo Scientific) and gene-specific primers (5 μM of forward and reverse primers) ...
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bioRxiv - Biochemistry 2019Quote: ... For MS analysis proteins were run for 1-2 cm on a 4-12% PAGE (NuPAGE Bis-Tris Precast Gel, Life Technologies) and stained with silver (SilverQuest Silver Stain ...
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bioRxiv - Synthetic Biology 2019Quote: ... Toehold strands were diluted to 1011 strands and mixed with biotinylated oligos at a ratio of 1:40 in a 50 µL reaction containing 2 mM MgCl2 (Invitrogen, Y02016) and 50 mM KCl (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Cells seeded on 13 mm coverslips placed in 24 well plates were transfected twice (on day 1 and 2) with Oligofectamine or RNAi MAX (Thermo Fisher) complexed with 20pmol of each indicated siRNA and analyzed 3 to 4 days after the first transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... Functional hair cells of neuromasts were stained using 2 µM solution of Yo-PRO-1 iodide (Thermo Fisher Scientific, cat. #Y3603) in 1x Holt’s buffer for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... before incubation (2 h at room temperature) with a mixture of AlexaFluor 488 or 568 goat anti-rabbit secondary (1:500, Life Technologies) and biotinylated horse anti-mouse secondary (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and then for 1 h at room temperature in 2 µg/mL Alexa 488-conjugated donkey anti-rat antibody (A-21208, Thermo Fisher). Slides were mounted with ProLong Gold containing DAPI (P36931 ...
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bioRxiv - Biophysics 2021Quote: ... qPCR was carried out in technical triplicates with 2 µl of 1:40-diluted cDNA per 10 µl reaction using SYBR Green PCR Mastermix (Applied Biosystems) with a final primer concentration of 500 nM ...
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bioRxiv - Immunology 2021Quote: ... ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher) was determined by AmplexRed assay [3] ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS then suspended in goat anti-mouse IgG (H&L) cross-adsorbed secondary antibody Alexa Fluor 488 (2 mg mL−1; Thermofisher Scientific) in PBS 0.1 % BSA for 45 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: Sections were rinsed three times in PBST and incubated for 2 hrs at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Three washes in PBST were performed ...
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bioRxiv - Cancer Biology 2020Quote: ... PBMC were maintained in complete RPMI 1640 and treated directly with MEDS433 alone or with Dypiridamole (0.1–1 μM) or stimulated with 2% phytohemagglutinin (PHA, Gibco, Thermo Fisher Scientific), IL2 200U/ml (Peprotech ...
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bioRxiv - Biophysics 2020Quote: 106 T-cells were incubated in T-cell media supplemented with 5 μg ml−1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Neuroscience 2021Quote: ... Washed cells were incubated with PBS2+ containing 2 mg mL−1 Pierce Premium Grade Sulfo-NHS-SS-Biotin (PG82077; Thermo Scientific) for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections containing infarct core and penumbra were blocked with 5% BSA in TBS with 0.5% v/v Triton X-100 (TBS-T) and incubated with anti-MAP-2 (1:200; Invitrogen, PAS-17646) and anti-TUJ-1 antibodies (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Three hours after plating medium was exchanged for maintenance medium (Neurobasal-A without Phenol Red, 2% B-27 supplement, 1% penicillin-streptomycin, 0.5 mM glutaMAX, 25 μM β-mercaptoethanol; ThermoFisher Scientific) and cells were kept at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 h at RT in the secondary antibody (1:1000, goat anti mouse Alexa Fluor 647, Life Technologies) carrier solution (1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in 3×10 min in 0.1% PBT and kept in the same solution in the fridge until mounted (1 or 2 days later) and coverslipped with ProLong Diamond with DAPI (Thermo Fisher). Cells were imaged using a confocal microscope (Nikon A1si ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in 3×10 min in 0.1% PBT and kept in the same solution in the fridge until mounted (1 or 2 days later) and coverslipped with ProLong Diamond with DAPI (Thermo Fisher).
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bioRxiv - Cancer Biology 2021Quote: ... the medium was replaced with 1 mL of fresh medium with 2 drops of NucBlue LiveProbe reagent (Thermo Fisher; Cat#: R37605). Live imaging was performed on a Leica DMI8 microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-2 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Gene expression in mouse cells was quantified using specific primers from Life technologies for mGAPDH (Mm99999915_g1) ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA at a concentration of 1-2 mg/ml was mixed with 5 µl of Salmon Sperm DNA (10mg/ml) (Thermofisher Scientific) prior to electroporation ...
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bioRxiv - Cancer Biology 2020Quote: ... The sections were then incubated with Alexa Fluor 488 antimouse and the Alexa Fluor 546 F(ab’)2 fragment of goat antirabbit IgG (HþL) (Invitrogen; 1:2,000) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... GST-COSA-1 and CDK-2-6xHis were co-expressed and purified from Sf9 cells using the Bac-to-Bac system (Life Technologies). Infected High-Five cells (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... About 1-2 μg total RNA was reverse-transcribed into cDNA using the SuperScript™ III First-Strand Synthesis System (Invitrogen). Qualitative PCR was performed with GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2020Quote: ... 10 μl of the resulting 1:10 dilution were mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher) and subjected to library preparation for next-generation sequencing (vertis Biotechnologie) ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA levels were quantified by quantitative reverse-transcriptase PCR (qRT-PCR) specific for the SARS-CoV-2 E gene (23) using TaqMan Fast 1-Step Master Mix (Applied Biosystems) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Microbiology 2020Quote: ... The buffer with dissolved gel slice was added to 1 mL ice-cold 100% ethanol and 2 μL of glycogen (20 mg/ml, ThermoFisher, R0561) and incubated at −20°C for at least one hour ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using ketamine / xylazine and intraviteally injected with 2 μl AF488 cholera toxin subunit B (1 mg/ml in PBS) (ThermoFisher Scientific). After 72 h mice were anaesthetized and euthanized via 4% PFA cardiac perfusion ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed three times in PBS – 0.05% saponin and incubated for 2 h with a donkey anti-goat Alexa Fluor® 488 (1/1000, Invitrogen A11055) diluted in PBS – 0.05% saponin – 0.2% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were left at room temperature for 24 hours and run in a 2% agarose gel containing 1/10000 SYBR Gold (ThermoFisher S11494) at 90V for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... ticks from each pool were put into a 2 mL centrifuge tube containing 1 mL of Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) prior to being homogenized in a frozen grinder (Jingxin ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in water at a desired concentration of 1-2 ug/uL as measured by a Qubit 3.0 dsDNA assay (Thermo Fisher Scientific). This product was subsequently used as the template for a dsRNA synthesis reaction (MegaScript RNAi ...
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bioRxiv - Cancer Biology 2020Quote: ... Harvested cells were spinned at 400g for 5 min and pellet was suspended at 1×106 cells per ml in pre-warmed DMEM (supplemented with 2% serum) media (Gibco, Invitrogen) in two tubes ...
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bioRxiv - Genomics 2021Quote: ... Cells were then quickly washed with 2 ml of PBS followed by permeabilization with 1 ml of PBS with 0.1% Triton X-100 (Thermo Fisher, 28314) for 10 min at room temperature ...