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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... beads are collected and reverse transcribed with MMLV reverse transcriptase (Maxima RT, Thermo Fisher), unused primers are degraded with Exonuclease I (New England Biolabs) ...
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bioRxiv - Molecular Biology 2019Quote: ... from cDNA from Huh7 cells prepared with the Superscript III RT kit (Thermo Fisher) using the oligo(dT)20 primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription was performed using the RevertAid RT Reverse Transcription Kit (ThermoFisher Scientific, USA). Quantitative PCR analysis of gene expression levels was performed in a CFX96 Touch thermocycler (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed into cDNA by a high-capacity cDNA RT kit (#4368813, ThermoFisher). Quantitative PCR reactions were run with Fast Universal PCR master mix (#4352042 ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription (RT) was performed using the SuperScript VILO cDNA synthesis kit (Life Technologies) with 650ng of input RNA per 80μl reaction ...
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bioRxiv - Genomics 2020Quote: ... followed by HRP-conjugated secondary antibody incubation at RT using ECL (Thermo-Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... RT-qPCR analysis was performed using a TaqMan gene expression assay (Thermo Fisher Scientific) with the following specific primers ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation for 20 min at RT in 100 µl culture medium (DMEM (Invitrogen) with 25 % HBSS (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 2 hours at RT with strepavidin–HRP (Invitrogen; Cat #434323). Sections were washed twice in 0.1M phosphate buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNA levels were ascertained by RT using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) real time quantitative PCR using sybr-green (Kapa Biosystems ...
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bioRxiv - Bioengineering 2020Quote: The RT-qPCR assay was performed using the ABI 7500 Fast DX (Applied Biosystems) instrument ...
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bioRxiv - Cancer Biology 2020Quote: ... Final concentrations of components of RT reaction were: dNTPs 0.25mM (Invitrogen or INBIO Highway); DTT 10mM (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems) on a StepOnePlus™ Real-Time PCR System (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed by using Fast SYBR™ Green Master Mix (Applied Biosystems) with Step One Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was done using PowerUp™SYBR™Green Master Mix (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthetized from 1 mg of total RNA using M-MLV RT (Invitrogen). cDNA samples from 1:5 dilutions were used for real-time PCR reactions ...
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bioRxiv - Microbiology 2019Quote: ... RT products were quantified by Taqman qPCR using TaqMan Gene Expression Master Mix (ThermoFisher) and primers and probe specific to GFP ...
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bioRxiv - Cancer Biology 2020Quote: ... 48 hours post-RT cells were stained with 4,6-diamidino-2-phenylindole (DAPI; Invitrogen). The slides were washed twice with PBS and mounted with Vectashield (Vector Labs) ...
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bioRxiv - Cell Biology 2021Quote: ... The RT-qPCR reaction was performed using the SYBR green detection reagent (Applied Biosystems) in StepOne Real-Time PCR System machines using the StepOne software v2.3 (Applied Biosystems) ...
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bioRxiv - Genetics 2022Quote: ... and RT was performed with the RevertAid H Minus Reverse Transcriptase (Thermo Scientific, EP0452) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... amplification was performed in a RT-qPCR IQ5 System (Applied Biosystems, Foster City USA) with the following parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... the premixed solution containing 4 µl of 5×RT buffer (Invitrogen, Cat no. #18080044), 2µl of 0.1 M DTT (kit component of Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... RT-qPCR reactions were performed using PowerUp SYBR Green Master Mix (Applied Biosystems, A25742), following to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... for 10 min at RT and mounted in PermaFluor mounting medium (Thermo Scientific, UK). Slides were scanned using an LSM 710 confocal microscope and ZEN 2009 software (Carl Zeiss Ltd ...
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bioRxiv - Microbiology 2020Quote: ... Thermal cycling conditions were adapted from Verso 1-step RT-qPCR kit (ThermoFisher, AB4101C) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... was used for cDNA synthesis by using SuperScritRIII RT First Strand Synthesis Kit (Invitrogen) according to its protocol ...
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bioRxiv - Microbiology 2020Quote: ... were quantified by RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and specific primers and probes (Supplementary Table 3) ...
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bioRxiv - Molecular Biology 2020Quote: Digital RT-dPCR was performed in a QuantStudio 3D™ System (Applied Biosystems™), using QuantStudio™ 3D Digital PCR Master Mix v2 dPCR mastermix and QuantStudio™ 3D 20K v2 chips ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200 ...
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bioRxiv - Bioengineering 2020Quote: ... RT-qPCR was done using the TaqMan Gene Expression Master Mix (Applied Biosystems, 4369016) in a QuantStudio 7 Real Time-PCR system ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The RT-qPCR was performed with TaqMan Gene Expression Master Mix (Life Technologies, USA) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: Sections were incubated for 10 min at RT in Neurotrace 660 (Molecular Probes, Invitrogen) dil ...
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bioRxiv - Neuroscience 2022Quote: The reaction buffer of the RT-QuIC consisted of 1 mM EDTA (Life Technologies), 10 μM thioflavin T ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: Sections were incubated for 10 min at RT in Neurotrace 660 (Molecular Probes, Invitrogen) dil ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription (RT) was performed with RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific). RT-PCR was conducted on BioRad C1000 Touch Thermal Cycler system with Phusion High Fidelity (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... Crude lysate was used for RT with Maxima H Minus Reverse Transcriptase (Thermo Scientific). Ten oocytes from the same mouse were used for a control RT minus reaction ...
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bioRxiv - Microbiology 2019Quote: The total RNA extracted from samples was randomly reverse transcribed using SuperScriptII RT (Invitrogen) at 42°C ...
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bioRxiv - Genomics 2019Quote: ... RT-qPCR was performed in technical duplicate with SYBR Select Master Mix (Applied Biosystems) using 10 ng of cDNA and 400 nM forward and reverse primers ...
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bioRxiv - Bioengineering 2019Quote: ... RT Enzyme Mix (ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor, Thermo Fisher Scientific) and PVinterF and YanRA primers [32] ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCR analysis was carried out using SYBR Green Master Mix (Thermo Fisher, USA). The sequence of the primers used to detect these genes are listed in Supplemental Table 1.
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bioRxiv - Genomics 2019Quote: ... Random amplicons were obtained using SuperScript III RT and HiFi Taq (ThermoFisher, Waltham, MA). Enhanced sensitivity in amplification was pursued utilizing microfluidic amplification with DENV-2 specific and random primers and SSIII/HiFi Platinum Taq on the integrated fluidic circuits (IFC ...
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bioRxiv - Microbiology 2019Quote: ... RNA was transcribed to cDNA with the High Capacity cDNA RT kit (Applied Biosystems). qPCR was performed as described before [8] ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purified RT-DNA was then analyzed on 10% Novex TBE-Urea Gels (Invitrogen), with a 1X TBE running buffer that was heated >80C before loading ...
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bioRxiv - Zoology 2020Quote: ... 6.25μL of 4X RT-Buffer (TaqMan Fast Virus 1-Step Master Mix, ThermoFisher Scientific), 600nM of the forward primer (RdRp_SARSr-F) ...
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bioRxiv - Cancer Biology 2021Quote: ... The EMT arrays and subsequent validations were performed on ViiA7 RT-qPCR System (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... closed packed barcoding beads in RNA bead buffer (1x Maxima H-RT Buffer (ThermoFisher), 2% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using EXPRESS Sybr GreenER 2X master mix with ROX (Invitrogen) and primers homologous to Shh ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed using Fast Sybr Master Mix (Thermo Fisher Scientific Inc., USA) in a final reaction volume of 10 µL ...
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bioRxiv - Microbiology 2020Quote: ... The RT-reaction was performed in Veriti™ 96-Well Thermal cycler (Applied Biosystems) (5 min at 25C ...
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bioRxiv - Bioengineering 2021Quote: ... RT-qPCR reactions were performed with PowerUp SYBR Green Master Mix (Applied Biosystems, A25742), according to manufacturer’s instructions ...