Labshake search
Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
-
bioRxiv - Neuroscience 2023Quote: ... a goat anti-mouse Alexa-488 conjugated antibody was applied (1:1000) for 2 h at room temperature (Life Technologies). Finally ...
-
bioRxiv - Plant Biology 2023Quote: RNA in situ hybridization was conducted using Invitrogen ViewRNA ISH Tissue 1-Plex and 2-Plex Assay kits (ThermoFisher Scientific) using the manufacture’s protocol optimized for Medicago root and nodules sections (Kulikova et al. ...
-
bioRxiv - Physiology 2023Quote: ... Tissues were then rinsed 2× in PBS and incubated in Bodipy FL Dye (1:500 dilution from a 1mg/mL stock, ThermoFisher) for 30min in PBS ...
-
bioRxiv - Plant Biology 2023Quote: ... were co-transformed with the plasma membrane marker PM-yk, endoplasmic reticulum ER-gk (Nelson et al., 2007) or stained with 2 µg mL-1 DAPI (D1306; Invitrogen) to stain nuclei ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were washed three times in PBS and then incubated for 2 hr at room temperature in Alexa Fluor 488 goat anti-chicken IgG (1:1000, Invitrogen), or Alexa Fluor 594 goat anti-chicken IgG (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... γ-TuRC was biotinylated prior to elution by incubation for 1 hour on ice in CSF-xB 2% (w/w) sucrose supplemented with 40 μM NHS-PEG4-biotin (ThermoFisher). The elution was concentrated using a 100 kDa MWCO spin concentrator (Amicon) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were washed three times with PBST followed by incubation for 1-2 hrs at room temperature with appropriate secondary antibody conjugated to Alexa 647 (ThermoFisher) diluted at 1:1000 in antibody dilution buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... sample wells were charged with a 100 µL of purified Heparin-BSA or rHS-BSA solution (1 – 2 ng/µL) in DPBS++ (Gibco), and the plates were rocked overnight (16 – 24 hours ...
-
bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
-
bioRxiv - Cancer Biology 2023Quote: Ex vivo cultures from primary tumor explants were generated by mechanical dissociation and incubation for 1 hour at 37°C with a solution of collagenase IV/dispase (2 mg/ml) (Invitrogen), resuspended in DMEM (Lonza ...
-
bioRxiv - Neuroscience 2023Quote: ... The digestion solution was then replaced with 1 ml HABG (97.75% Hibernate A Low Fluorescence [BrainBits, HALF], 2% B27 supplement [Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... the reaction mix was cooled to 4°C and analyzed by standard agarose gel electrophoresis with 2% (w/v) Agarose in TAE Buffer with 1:20.000 SybrSafe (Thermo Fisher), followed by imaging with Odyssey Fc at 600nm.
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... the larvae were exposed to 0.25% sodium dodecyl sulfate (SDS) for 2 min to permeabilize the cuticle followed by 1 h incubation with 10 µg/mL Hoechst 33342 (ThermoFisher) or 30 µM DRM oligoTEA linker and 10 µg/mL Hoechst 33342 ...
-
bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
-
bioRxiv - Immunology 2023Quote: MD39 trimer immunogens bearing a free C-terminal cysteine at a concentration of 1 mg/ml were reduced by incubation with 10 molar equivalents of tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher), followed by incubation at 25°C for 10 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the DNA template was digested by adding 1 µl of TURBO™ DNase (2 U/µL) (Thermo Fisher Scientific) and incubating for 30 min at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Subsequent dissociation was performed in reduced serum media (DMEM media; #41966 with 2% FBS; #10270106, 1% Pen-strep; #15140122, and 25mM HEPES; #15630056, all products from Gibco) supplemented with Collagenase IV (1mg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... Differentiation was initiated by changing media to N2B27-RA media (2:1 ratio of DMEM/F12 and Neurobasal medium (Gibco), +1% L-glutamine ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... for cells cultures or 2% (v/v) SVF for parasites cultures (Biowest, France) and supplemented with 1% antibiotics (penicillin-streptomycin) (GIBCO).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and perilipin-2 was stained with Alexa Fluor™ 488 goat anti-rabbit IgG (1:500, # 11034, Lot: 10729174; Invitrogen) for 30 min (RT).
-
Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... and after 24-hour the medium was replaced with serum-free advanced DMEM supplemented with 2 mM L-glutamine and 1% antibiotic-antimycotic (Gibco). After incubation for 48 hours ...
-
bioRxiv - Genetics 2024Quote: ... tissue samples were incubated for 2 h with 1 ml of an antibiotic solution containing Gentamicin (50 µg/ml Gibco) and PrimocinTM (50 µg/ml ...
-
bioRxiv - Immunology 2024Quote: ... anti-mouse and anti-rabbit secondary antibodies was applied with DAPI nuclear stain (4’6-diamidino-2-phenylindole, 1:500, #62248, ThermoFisher Scientific). All reagents were prepared in multistaining buffer (BU06 ...
-
bioRxiv - Developmental Biology 2024Quote: ... sections or cells were incubated with bisbenzimide (1:100 in 0.1M PB) for 2 min at room temperature and mounted using Fluoromount-G (Thermo Fisher).
-
bioRxiv - Cell Biology 2024Quote: ... Egg chambers were washed 3X 20 min in PBST and fixed in 4% PFA followed by 3X 10 min washes in PBST preceding a 2 hr incubation in a fluorescently labeled secondary (1:1000; Dylight 550 or 650; ThermoFisher). Washed 3X for 10 min in PBST and mounted as previously described ...
-
bioRxiv - Cell Biology 2024Quote: ... Switzerland) and Hoechst 33342 dye (1 drop/2 ml of NucBlue Live ReadyProbes Reagent, Thermo Fisher Scientific, Waltham, MA, USA) and have been confirmed to be mycoplasma free.
-
bioRxiv - Molecular Biology 2024Quote: ... Roughly 2 mg of purified Tfb3 was covalently linked to 1 ml AminoLink® Plus Coupling Resin (Thermo Scientific #PI20505) following the manufacturer’s protocol for crosslinking at pH 10 with cyanoborohydride solution ...
-
bioRxiv - Neuroscience 2024Quote: ... in a solution of PBS with 0.2% Triton–X100 and tissue sections were incubated with goat anti-eYFP (Anti-GFP chicken IgY fraction 1:1000, Invitrogen) and anti-c-Fos (Anti-c- Fos rabbit ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were imaged in non-fluorescent culture medium (FluoroBrite 4.5 g/l Glucose, 1% PenStrep, 2% FBS, L-Glutamine from Life Technologies). For secretion and capture ...
-
Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... and the medium was then replaced with serum-free advanced DMEM supplemented with 2 mM L-glutamine and 1% antibiotic-antimycotic (Gibco). After incubation for 48 hours ...
-
bioRxiv - Physiology 2024Quote: ... hearts were loaded with 1 mg/ml Rhod-2 AM in 0.5 mL of DMSO containing 10% pluronic acid (Molecular Probes) followed by 50 µL 1 mg/ml RH237 in DMSO (Molecular Probes) ...
-
bioRxiv - Immunology 2024Quote: ... Plasmids encoding antibody heavy and light chain genes were used to transfect suspension Expi293F cells in a 1:2 heavy to light chain ratio using ExpiFectamine 293 transfection reagents (Gibco) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... DRGs cultures were grown at 37 °C in Neurobasal-A medium supplemented with 2% B-27 and 1% Glutamax-I (Gibco).
-
bioRxiv - Microbiology 2024Quote: ... 1% hematocrit) were resuspended in binding medium (RPMI 1640 medium, 2% glucose, pH 7.2; Gibco, Thermo Fisher Scientific, Bremen, Germany) and co-incubated with HBEC-5i cells (70-90% confluence ...
-
bioRxiv - Neuroscience 2024Quote: ... PolyA+ RNA-seq libraries were prepared from 500 ng total RNA input supplemented with 2 μl 1:200 ERCC RNA Spike-In Mix (Invitrogen) using the TruSeq Stranded mRNA HT Kit (Illumina ...
-
bioRxiv - Molecular Biology 2024Quote: ... All samples were then incubated with DAPI (4’,6’-diamidino-2-phenylindole) at 1 ug/ml in PBS to visualize DNA and mounted in Prolong Gold mounting media (Invitrogen). Images were acquired on the Zeiss LSM 980 microscope.
-
bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
-
Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
-
bioRxiv - Synthetic Biology 2024Quote: ... inserts were fabricated using 3D-printed resin molds and 1 (or 2) mm biopsy punches (Fisher Scientific #12-460-402). Printed molds were washed with isopropanol after printing ...
-
bioRxiv - Microbiology 2024Quote: ... 750 mM aminocaproic acid, 2 mg/ml lysozyme, 1 mM EDTA, 1.43 mM BME, 1x EDTA-free Pierce Protease Inhibitor [Thermo Scientific]) and incubated 20 min on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... were cultured in complete DMEM (10% Tetracycline free FBS, 1% Penicillin-Streptomycin) including 2 mg/ml G418 (Generon) and 50ug/ml hygromycin (ThermoFisher). All cell lines were screened every three months for mycoplasma infection.
-
bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... pCG1-SARS-2-SdC19 or pCG1-SARS-2-SdC19-H2 using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instruction ...
-
bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells (70–80% confluent) were loaded with 2 μM Fura 2 AM (Molecular Probes, Oregon) in culture medium for 20 min at 37 °C in a humidified atmosphere of 95% O2 and 5% CO2 ...