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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged as small clumps every 4 to 5 days with Dispase (Gibco). All cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2024Quote: ... Intracellular Ca2+ ([Ca2+]i) measurements were conducted using Fluo-4/AM (5 µM; Invitrogen)-loaded cells ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (Termo Fisher Scientific, 90,115) for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged as small clumps every 4 to 5 days with Dispase (Gibco). All cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (Thermo Fisher Scientific, 90115) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... 94°C 5 min) with 4 μl of RNA and random hexamers (Thermofisher scientific). A PCR was further performed based on the protocol established by Tiller et al (Tiller et al ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 5 µg/ml NeutrAvidin (Thermo Fisher Scientific), then blocked in PBS containing 1% BSA for 1 hour at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... muscles were injected with 5 µM Fluo-4 penta-potassium salt (ThermoFisher Scientific, USA) as previously described and viewed with DIC optics on a Nikon Eclipse TE300 inverted light microscope (400× ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections of 4-5 μm were cut using a rotary microtome (HM355S, Thermo Scientific). Sections were deparaffinized in xylene and rehydrated in a descending series of ethanol (96%–50% ...
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bioRxiv - Microbiology 2024Quote: ... After 4–5 days of culture in the Expi293 Expression Medium (Thermo Fisher Scientific), supernatants were collected and passed through a 0.22-µm filter ...
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bioRxiv - Neuroscience 2023Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (Thermo Fisher Scientific, 90115) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was exchanged on DIV 4-5 for phenol-free Neurobasal medium (Thermo Fisher) supplemented with GlutaMAX 1x (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cultured at 37 °C and 5 % CO2 on 4-well plates (Nunc) (300 µl/well ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using 7-AAD (7-Aminoactinomycin D, Thermo Fisher Scientific, 00-6993-50). Cells were analyzed using a FACSCanto II flow cytometer (BD ...
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bioRxiv - Cell Biology 2021Quote: ... Each RNA sample (1 μg) was subjected to reverse transcription (Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase, Invitrogen™ 12574018), and then amplified by real-time PCR (Ssoadvanced™ Universal SYBR ...
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bioRxiv - Bioengineering 2019Quote: ... The bionic corals were transferred to 6-well plates filled with 3 mL of ASW medium30 containing broadband antibiotics (penicillin/streptomycin, Gibco) at a concentration of 1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: 4T1 and 67NR cells at 70-80% confluence were transfected with the shRNA constructs described above in FuGene 6 at a ratio of 3:2 per manufacturer’s instructions (Thermo Fisher). Pools of transfected cells were selected in media with 7 µg/ml puromycin ...
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bioRxiv - Genomics 2021Quote: Organoids seeded in 6-well plates were harvested after 3-days incubation with WYE-132 in RIPA buffer (Thermo Scientific) supplemented with protease inhibitors (Complete Mini ...
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bioRxiv - Cancer Biology 2022Quote: ... 1µM PRMT5 inhibitor or negative control treated (3 or 6 day) OCI-AML-20 cells were incubated with 10 µM EdU (Invitrogen) for 2 h at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Immunology 2022Quote: ... primers are listed in Supplemental Table 3), Luminex using a custom procarta Luminex 6-plax-cytokines (IL1β, IL6, IL8, IL18, CCL2, and TNFa) detection kit (ThermoFisher) (Supplemental Fig ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples (12 μL) were mixed with 3 μL 5 M DTT and 5 μL 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and incubated (95 °C ...
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 3.5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Cell Biology 2020Quote: ... The cells were then stained with secondary antibodies diluted 1:500 in 3% BSA and DAPI (Invitrogen D3571) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Constructs were incubated for 1 hour at 37°C with 3 mM Fluo3 AM calcium indicator (ThermoFisher Scientific) in differentiation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Iba1 and caspase-3 were labelled with goat anti-rabbit Alexa Fluor 555 (Invitrogen, A21429; 1:500 dilution), and GFAP and HSP were labelled with goat anti-mouse Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-cleaved caspase 3 secondary antibody (goat anti-rabbit Alexa Fluor 647, Thermo Fisher; 1:400) and anti-Ecadherin secondary antibody (goat anti-mouse Alexa Fluor 555 ...
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bioRxiv - Bioengineering 2020Quote: ... the collagen solution was prepared by mixing 50% (v/v) of 3 mg/mL type 1 collagen (ThermoFisher) with 2.5% of 1N NaOH ...
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bioRxiv - Physiology 2019Quote: ... and 1 µL of either dystrophin (Dmd) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe/primer mix (Applied Biosystems; Mm00464475_m1 and Mm99999915_g1 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were transfected with constructs (co- transfections were performed at 1:3 ratio) using Lipofectamine 2000 (Invitrogen), as described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... D3 was cultured in DMEM/F12 (3:1) medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 8 ng/mL Cholera Toxin (CELL technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... which was performed using a 1:3 ratio of 100 nmol/L siRNAs with Lipofectamine 2000 reagent (Invitrogen) diluted in OptiMEM (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μg of extracted proteins (n = 3) were solubilized in 1 × NuPage® LDS Sample buffer (Invitrogen, USA) and was run in a 15 well 12% Tris-Gly precast gel (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was stopped by ethanol precipitation: 1 μl 3 M RNAse-free sodium acetate (pH 5.5) (Ambion), 1 μl RNAse-free glycogen (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Braun) were cultured in 3 parts DMEM and 1 part Ham’s F-12 Nutrient Mix (Thermofisher scientific 11765054), supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of DNA was added to 100 μL of optiMEM as well as 3 μL Lipofectamine (Invitrogen) to a separate 100 μL of optiMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... Coverslips were washed 3 times with PBS before the Alexa fluor secondary antibody (1 in 500) (Fisher Scientific) was added and the cells incubated for a further 1 hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:3 when they reached 80% confluence by harvesting cells with 0.25% trypsin EDTA (Gibco).
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bioRxiv - Immunology 2022Quote: ... 1-3 million cisplatin-treated cells were thawed and transferred into Nunc 96 DeepWell polystyrene plates (Thermo Fisher). After two washes with Barcode Perm buffer (Fluidigm) ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron BA.1 (B.1.1.529) were propagated to passage three on Calu-3 cells in Advanced DMEM/F12 (Gibco), supplemented with HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2 mg/ml proteinase-K (Invitrogen; 25530049) for 10 min at 55 °C ...
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bioRxiv - Cell Biology 2021Quote: ... blocked and stained for F-actin (phalloidin-Alexa Fluor 647; Invitrogen; 1:100 in 3% BSA in PBS) for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... followed by another 3 washes with DPBS and incubation in 1:500 Alexa Flour 488 (ThermoFisher Scientific, A32723) and 1:10000 DAPI (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... blocked with 3% BSA and incubated with the following primary antibodies: rabbit anti-CD31 (1:50, PA516301 Invitrogen), mouse anti-GLUT1 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 times) and immediately incubated for 30 min with streptavidin–peroxidase complex diluted 1:400 in TBS (Invitrogen). The chromogen was 3-30-diaminobenzidine (Vector Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μg plasmid and 1.5 μl Fugene HD (m/v=1:3) were diluted and incubated in 0.25 ml OptiMEM (Gibco). After 5 min ...