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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed with ACK Lysing Buffer (Gibco, 3 ml for 5 min). The cells were counted and resuspended in RPMI-1640 medium (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... digestion for 3 to 5 min at 37 °C and washed with Neurobasal medium (Invitrogen) supplemented with 2% B-27 (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... The neurons were then washed 3 times for 5 minutes with 1X DPBS (14080055, Gibco) containing 0.1% Tween ...
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bioRxiv - Microbiology 2023Quote: ... FungiQuant-Prb (6FAM) 5′-TGGTGCATGGCCGTT-3′ (MGBNFQ) 57 and QuantStudio3 instrument (Applied Biosystems, CA, USA). We used the following qPCR conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... and then 3 hours at 37°C with 5 μg/mL mouse laminin (Thermo Fisher). Neurons are cultured in BrainPhys neuronal medium (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... Fluorescence was measured using a QuantStudio 3 or QuantStudio 5 qPCR machine (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... 5 × 106 cells were cultured in 3 ml Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (Life Technologies).
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bioRxiv - Neuroscience 2024Quote: ... 3-5 μL of a heavy-weight dextran (Invitrogen, Cat#D1818, 70,000 MW, lysine fixable) was injected into each juvenile octopus and allowed to circulate throughout the body for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... GAPDH reverse: 5′-AAG TGG TCG TTG AGG GCA ATG -3′ (Invitrogen, Thermo Fisher Scientific); ALIX forward ...
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bioRxiv - Bioengineering 2024Quote: ... GAPDH reverse: 5′-AAG TGG TCG TTG AGG GCA ATG -3′ (Invitrogen, Thermo Fisher Scientific); ALIX forward ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... and the cells resuspended in 3-5 mL of pancreatosphere medium (DMEM/F12 (Gibco, #11320033), 1% P/S ...
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bioRxiv - Plant Biology 2024Quote: ... Cells were disrupted by passing them 3-5 times through a French press (Thermo Fisher) and centrifuged for 50,000g for one hour at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... slices were fixed with 4% paraformaldehyde at 4°C overnight and incubated with Alexa 647 coupled-streptavidin (Invitrogen S21374, 1:500 in PBS-10% tween) for 6 h at room temperature ...
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bioRxiv - Genomics 2023Quote: ... the ORF4-6 genomic region was amplified directly from the clinical samples using the SuperScript™ IV One-Step RT-PCR System (Thermo Fisher Scientific, Waltham, MA). Primers were designed using Primer3 (26 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were then incubated for one hour in Alexa-conjugated secondary antibodies and/or F-actin marker Alexa-conjugated Phalloidin (Life Technologies; 1:1.000) or Rhodamin-labeled Phalloidin (SIGMA ...
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bioRxiv - Cancer Biology 2022Quote: ... were harvested and one part of the metastatic tissue (1 cm in diameter) was placed in ice-cold PBS (ThermoFisher Scientific, Waltham, MA) containing gentamicin (50 ng/ml ...
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bioRxiv - Microbiology 2019Quote: ... The DNA was dissolved in 50 μl molecular grade water for 1 hour at 65°C and the DNA concentration determined using a NanoDrop™ One/Onec Microvolume UV-vis Spectrophotometer (ThermoFisher Scientific, USA).
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bioRxiv - Physiology 2021Quote: ... Globin transcripts were then amplified from 1 μg of extracted RNA using the One-Step RT-PCR system with Platinum Taq DNA polymerase High Fidelity (Invitrogen, Carlsbad, CA, USA). PCR cycling was performed with 1 cycle at 50 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% Triton X-100) and then incubated for one hour with 1:10000 diluted monoclonal mouse anti-HA antibody (#26183, ThermoFisher Scientific, MA, USA) in PBS-T ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was incubated for one hour with 1:10000 diluted horseradish peroxidase-coupled goat anti-mouse antibody (#62-6520, ThermoFisher Scientific, MA, USA) in PBS-T and washed again five times for 15 minutes with PBS-T ...
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bioRxiv - Genomics 2020Quote: ... For qPCR we performed one-step multiplex real time PCR using TaqPath™ 1-Step Multiplex Master Mix (ThermoFisher Scientific, Cat. No. A28526) targeting 3 different gene-specific primer and probe sets - envelope glycoprotein spike (S) ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was performed using 1 µl of eluted RNA and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) as described by the manufacturer.
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times in 1X TBST and incubated with horseradish peroxidase-conjugated goat-anti-mouse or -rabbit secondary antibodies for one hour at room temperature (1:10,000, Invitrogen #31430, #31460, Rockford, IL). Membranes were subsequently washed three times in TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... and for one hour at room temperature with the following fluorescent secondary antibodies: goat anti-hamster IgG AlexaFluor 568 (Invitrogen; 1:500 dilution), goat anti-rabbit IgG AlexaFluor 647 (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Genomic PCR products obtained with primers 5’-CGCCATCAACTTCACCTAGC-3’ (forward) and 5’-TCTGGGAAGAAGTTTGGCCT-3’ (reverse) were sequenced using the BigDye® Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Massachusetts, USA) on an ABI 3130xl Genetic Analyzer (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... a probe prepared by PCR on genomic mouse DNA with the primers 5’-CATATTCCAGGTCCTTCAGTGTGC-3’ and 5’-CACTTTAGGACGTGAAAT ATGGCG-3’ was labeled with Cy3 by random priming according to the kit instruction (Invitrogen Kit, Ref 18095–011).
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bioRxiv - Bioengineering 2021Quote: ... sgRNA LA93527 was generated from a PCR DNA template (overlapping primers LA935 5’-GAAATTAATACGACTCACTATAGGACAGTGCGGTCCG-CAAGGGTTTTAGAGCTAGAAA-3’ and LA137 5’-AAAAGCACCGACTCGGTGCCA-CTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’) containing the T7 promoter using the MEGAscript T7 Transcription kit (ThermoFisher Scientific, Walthum, MA USA). The reaction mix was incubated at 37°C for 16 hours and purified using the MEGAclear Transcription Clean-Up kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated using either the Superscript 3 or 4 First Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... pET15b-CDA5 was amplified using primers 3 and 4 and added to the BsiW1 (Thermo Fisher) site of pSLIK using InFusion (Takara ...
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bioRxiv - Biochemistry 2022Quote: To prepare samples a 3:4 dilution was made in NuPAGE LDS sample buffer (ThermoFisher; NP007) supplemented with 10% v/v β-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... All PBMCs were stimulated for 3-4 days with 2ug/ml of phytohemagglutinin (PHA; Gibco BRL) and 1 ug/ml of interleukin-2 (IL-2 ...
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bioRxiv - Cancer Biology 2022Quote: 500000 cells in suspension were loaded with 3 μM Fluo-4 AM (Thermo Fisher Scientific, F14201) in DMEM without serum supplemented with 0.02% Pluronic-F127 (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Total RNAs for 3 to 4 independent experiments performed in triplicate were extracted by Trizol (Ambion) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and MCR:SATB2 (45-3 and 63-4) cell lines were cultured in DMEM medium (Life Technologies) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals) ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated DA neurons were incubated with 3 μM Fluo-4 AM (Thermo Fisher, USA, F14201) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and then for 3-4 h with 50 μM Click-iT® HPG (L-Homopropargylglycine) (Invitrogen). For gel electrophoresis analysis ...
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bioRxiv - Microbiology 2023Quote: ... during a 3-day culture followed by 4 days of incubation with 10% FBS (Invitrogen, USA) as previously described[43] ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 mL of the homogenized suspension was treated with 3 units/mL Rnase-free Dnase (Ambion) for one hour at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... at 4°C for 3 hours followed by addition of 10 ml Protein A Dynabeads (Invitrogen) for 20 min to capture the antibody-protein complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... CD44 and CD62L and then incubated with 5 μM Fluo-4 AM (Molecular Probes) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: SMN cultures were incubated at 37°C with 5 μM Fluo-4 AM (Invitrogen) for 30 min in Neurobasal A medium (no supplements) ...
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bioRxiv - Neuroscience 2020Quote: ... then 5 μg were loaded and separated on 4–12% NuPage acrylamide gels (Invitrogen) with NuPage MOPS running buffer for 2 h at 110 V ...
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bioRxiv - Microbiology 2021Quote: ... Lysates containing around 5 × 106 cells were separated on 4-20% polyacrylamide gels (Invitrogen) in running buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... the premixed solution containing 4 µl of 5×RT buffer (Invitrogen, Cat no. #18080044), 2µl of 0.1 M DTT (kit component of Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HIOs were cultured in group of 5 per well using 4-well plates (ThermoFisher). Lumens of individual HIOs were microinjected with glass caliber needles with 1 μl of PBS or different strains of Salmonella (105 CFU/HIO or 103 CFU/HIO for 24h time point experiments) ...
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bioRxiv - Immunology 2021Quote: ... 94°C 5 min) with 4 μl of RNA and random hexamers (Thermofisher scientific). A PCR was further performed based on the protocol established by Tiller et al (Tiller et al. ...
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bioRxiv - Neuroscience 2022Quote: ... were passaged every 4-5 days using Stem-Accutase (Cat# A11105-01, Life Technologies) in the presence of 1 µM Thiazovivin (THZ Cat# SML1045-25 MG ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-TLR-4 polyclonal antibody (5 µg/ml, PA5-23124, Thermo Fisher Scientific), and rabbit anti-TLR-5 polyclonal antibody (10 µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections of 4-5 µm were cut using a rotary microtome (HM355S, Thermo Scientific). Sections were deparaffinized in xylene and rehydrated in a descending series of ethanol (96%–50% ...