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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Chromatin-antibody complexes were captured for at least 3 hr using a mix of Protein A and G Dynabeads (Invitrogen). The captured immunoprecipitated complex were washed 10 min each with sonication buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were added for 3 h before 45 min incubation with protein A or G beads (Dynabeads 10002D, 10003D from Thermofisher scientific ...
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bioRxiv - Microbiology 2019Quote: ... bacterial cultures were centrifuged 3 min at 3000 × g and were resuspended in 10 µl of medium or washed in high purity PBS (GIBCO). 1 µl of this cell suspension was deposited on the pads ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018) using a dT primer and Superscript IV (Invitrogen) reverse transcriptase ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspension was pelleted (180 x g for 3 min) and re-suspended in differentiation media: GMEM (Glasgow’ s Minimal Essential Medium, Gibco), 5% KSR (knockout serum replacement ...
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bioRxiv - Immunology 2022Quote: ... the samples were mixed with Coomassie G-250 and resolved in 3-12% NativePAGE Bis-Tris gel (Invitrogen Catalog #BN1003BOX) as per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... 35-50 µg of solubilized membrane fractions were loaded onto pre-cast Blue Native-PAGE gels (3-12% Bis-Tris minigels, Invitrogen) and run using standard protocols138 ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 25 mins at 35,000 x g and the supernatant was adjusted to approximately 3 mg/ml protein (Nanodrop 2000 C, Thermofisher Scientific). To capture the SVs for content detection ...
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bioRxiv - Molecular Biology 2023Quote: ... 5-15 μg poly(A)-selected mRNA was fragmented for 3 minutes at 70°C using alkaline hydrolysis fragmentation reagent (AM8740, Ambion). Fragmented mRNAs were purified by performing a 1.8X bead cleanup with RNAClean XP beads (A63987 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5% Coomassie brilliant blue G-250 was added before loading ~100 μg on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... and aliquoted to 1 µg/µL in RNAse-free water at -80 °C for storage (concentration was determined using a Qubit 3 Fluorometer, Invitrogen).
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bioRxiv - Systems Biology 2024Quote: ... fresh medium was added to dilute the tryplE and cells were centrifuged at 400 x g for 3 min then washed twice with Phosphate Buffered Saline (PBS) (Gibco) with centrifugation in-between ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed 1:1 with addition of 1% B27 (Invitrogen), 0.5% N2 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... treated 1:1 with chloroform (Fisher Scientific, C606-1) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: We dissected brains and ventral nerve cords of 5-7 days old males in Schneider’s insect medium (Thermo Fisher Scientific, Waltham, MA) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2021Quote: ... For the analysis of STAT3/5 phosphorylation cells were also labeled with phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated anti-phospho-STAT3 (Tyr705) (Thermo Fisher Scientific) (#25-9033-42 ...
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bioRxiv - Biochemistry 2020Quote: ... QPCR was performed on standards of diluted genomic DNA and cDNA products using specific primers (Supplemental table 5) on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Galnts gene copy numbers were calculated using the genomic DNA as a standard curve and variation between biological replicate was normalized using Actb expression level.
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Neuroscience 2021Quote: ... Mg-ATP 4, Na2-phosphocreatine 10, Na3-GTP 0.2 (pH 7.3, 295 mosmol.kg-1) and 20 µM AlexaFluor488 (Invitrogen). Whole-cell patch-clamp recordings from identified interneurons 5IR_DIC ...
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bioRxiv - Cell Biology 2020Quote: ... Gonads were incubated overnight at 4°C with 1 U/ml Texas Red-X Phalloidin (ThermoFisher Scientific) in staining solution (1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated overnight at 4°C with antibodies directed against CD44 (Thermo Scientific, MA4400; 1:200) and CD56 (Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... ISF Aβ42 in the 14 samples for each mouse was measured by ELISA (Invitrogen, KHB3441, 1:4). To determine Aβ42 half-life (Cirrito et al. ...
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bioRxiv - Bioengineering 2019Quote: DSS-treated mice received 1% to 4% (weight/volume) DSS (40,000 MW, Alfa Aesar, Thermo Fisher, France) in drinking water for 5 days to induce an acute episode of colitis ...
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bioRxiv - Bioengineering 2020Quote: ... UK) and the piggyBac-BE3-GFP plasmid at a 1:4 molar ratio using Lipofectamine 3000 (Invitrogen). At 48 h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C and anti-mouse-HRP-coupled secondary antibody (1:3000, Life Technologies) was incubated for 1.5 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated overnight at 4°C with monoclonal primary anti-GFP antibody from rabbit (1:2,000) (Invitrogen), and with secondary anti-rabbit antibody (1:30,000 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were rinsed in PBS 4 × 20 minutes and then incubated in Hoechst (1:2000; Thermo Fisher) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed overnight at 4°C with monoclonal α-FLAG (#31430, ThermoFisher Scientific; 1:10,000) antibody diluted in 1×TBS-T buffer containing 3% bovine serum albumin ...
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bioRxiv - Physiology 2021Quote: ... The sections were then incubated overnight at 4°C with polyclonal anti-laminin (1:1000; ThermoFisher Scientific) primary antibody diluted in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% sodium bicarbonate) or selection medium (maintenance media supplemented with 4 μg/ml blasticidin (Thermo Fisher) and 2.5 μg/ml puromycin (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Genetics 2022Quote: ... at room temperature then incubated overnight at 4°C with mouse anti-v5 (1:1000, Invitrogen, RRID:AB_2556564), and rat anti-tubulin alpha (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... and for 48 hours at 4°C followed by 15 minutes of DAPI staining (1:5000; ThermoFisher). Retina whole mounts were also incubated with rabbit anti-Alexa-fluor 488 conjugated GFP for 4 days at 4°C followed by 15 minutes of DAPI staining ...
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bioRxiv - Biochemistry 2020Quote: ... or PAF (4 μM) with or without sAGP-1 using competitive ELISA kit (EMSCAMPL, Invitrogen, Vienna, Austria). This system displays a detection sensitivity of 0.39 pmol/ml of cAMP according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by goat anti-rabbit Alexa-488 (1:200, Invitrogen, Waltham, MA, USA) for 2 hours at room temperature and DAPI (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibodies overnight at 4 °C (1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The thrombin-selective inhibitor PPACK.2HCl and MMP Inhibitor V (ONO-4817 ...
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bioRxiv - Cell Biology 2019Quote: ... Neurons were cultured for 1-4 days in vitro (DIV) and transfected with Lipofectamine 3000 (L3000; Invitrogen) for 48 h (DIV 3-6 ...
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bioRxiv - Neuroscience 2019Quote: ... Wako #019-19741) followed by secondary antibody solution (4 h, RT, Alexa-Fluor 488, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Cell Biology 2021Quote: ... primary antibodies overnight at 4°C followed by Alexa Fluor-647 secondary antibodies (ThermoFisher Scientific, 1:500) for 2hrs at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... we seeded HEK 293 cells in a 4-chamber glass bottom dish (D35C4-20-1-N, Invitrogen). When the cells covered 70-80% of the chamber of a dish (almost 24 hours after seeding) ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples were mixed in a 1:4 ratio with 4X Native-PAGE sample buffer (ThermoFisher Scientific) and loaded into NativePAGE™ 3–12% Bis-Tris protein gels (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... PVDF membrane was incubated at 4 °C with 1:1000 ratio of PIKFYVE antibody (Invitrogen, PA5-13977) or 1:1000 ratio of α/β-Tubulin antibody (Cell Signaling ...