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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Supernatants (2 mg protein) were removed and incubated overnight at 4°C with Dynabeads Protein G (Thermo Scientific) coated with anti-FLAG antibody or IgG heavy chain according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... strains were negatively selected against the URA3 marker gene using 1 mg/mL of 5-Fluoroorotic Acid (5-FOA) (Fisher Scientific, F10501-5.0) in SD-plates ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... mice were first anesthetized with a ketamine (100 mg/kg) and xylazine (7 mg/kg) mixture then perfused with 4% paraformaldehyde (Thermo Fisher Scientific), and the brains were extracted and cryoprotected in 30% sucrose in 1X PBS until they sank ...
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bioRxiv - Microbiology 2021Quote: ... 7) and plunge-frozen at a condition of 95% humidity and 4°C by using a Vitrobot Mark IV (Thermo Fisher Scientific). The cryo-EM data were acquired with a Titan Krios at 300 kV (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... but the blastocysts were incubated in 10 μM 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC; Cell Tracker Blue, Life Technologies, Carlsbad, USA). Fluorescence intensity for GSH was measured under a Nikon scanning confocal microscope with a filter at 371 nm excitation and 464 nm emission ...
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bioRxiv - Neuroscience 2021Quote: ... with the substrate tertiary-butyloxycarbonyl-leucine-methionine-7-amino-4-methylcoumarin (abbreviated to t-BOC-Leu-Met-CMAC, or in short: CMAC) (Invitrogen, Carlsbad, CA), at a concentration of 50 μM in the culture medium ...
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bioRxiv - Physiology 2021Quote: Total RNA from around 20 whole flies of both sexes at 4-7 days old was extracted using a PureLink RNA Mini Kit (Cat# 12183020, ThermoFisher Scientific, USA). Reverse transcription (RT ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
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bioRxiv - Biochemistry 2024Quote: ... The grids were blotted using filter paper for 7 seconds at 4°C under 100% humidity using Vitro Mark IV (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced with a razor blade and subjected to a 30-minute enzymatic digestion at 37 °C in 3 mL of 1:1 DMEM/F12 media (Gibco, Grand Island, NY; 200 mM L-glutamine) with 1X-collagenase/hyaluronidase (10X stock ...
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bioRxiv - Cell Biology 2020Quote: ... larger regions of Lysozyme-immunostained ileal sections (4-8 mm for each animal) were scanned with an EVOS system (Invitrogen) and the total number of Lysozyme-positive epithelial cells per linear ileal length was manually quantified using FIJI ...
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bioRxiv - Cancer Biology 2020Quote: ... ATM and ATR in the CE samples as follows: the samples were loaded on 4-8% (w/v) Tris-acetate SDS-PAGE gels (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... We then added 8 μL/well of RT mix to the plate: 4 μL of 5x RT Buffer (Thermo Scientific), 0.35 μL of SUPERase-In RNAse Inhibitor (20 U/μL ...
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bioRxiv - Molecular Biology 2019Quote: Samples were loaded onto 8% SDS-polyacrylamide gels or 4–12% Tris/HEPES-SDS Bolt™ precast gels (Thermo-Invitrogen) for electrophoresis ...
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bioRxiv - Bioengineering 2021Quote: ... the mouse Achilles tendon samples were stained with 0.2% of 4 mg/ml fluorescein diacetate (FDA) (596-09-8, ACROS Organics) and 0.5% of 1 mg/ml propidium iodide (PI ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 4 million cells were plated in 10 cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then cells lysates were prepared using RIPA lysis buffer as described previously.30 Total proteins (8 µg/sample) were loaded onto 4%-20% Novex Tris-Glycine gels (Invitrogen), transferred onto polyvinylidene fluoride membrane ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000g for 30 min at 4°C with a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... for anti-eEF3 and anti-G-6-PDH secondary antibodies and developed with Pierce ECL plus western blotting substrate kit (Thermo Scientific 32132).
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bioRxiv - Biophysics 2019Quote: ... Cells were harvested by centrifugation at 7,800 g for 10 minutes in an F9 6×1000 LEX rotor (Thermo Scientific, Waltham MA), using a Lynx 6000 centrifuge (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were pelleted by centrifugation at 10628 g at 25°C for 10 min (Thermo Fisher Scientific, #F9-6×1000 LEX rotor) and washed once with sterilized water prior to re-suspension into (MM ...
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bioRxiv - Cell Biology 2022Quote: ... myotubes were transfected with 1µg of the MHC I (H2-kb) overexpression plasmid using Lipofectamine™ 2000 reagent for a period of 6 hours (Invitrogen, USA), both without and with the addition of the ER stress blocking agent ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Microbiology 2023Quote: Epimastigotes and trypomastigotes lysates containing 2 x 107 parasites in 20 µL of SDS-PAGE sample buffer containing 5 mM DTT were boiled for 5 min and loaded onto precast Novex Value 4-12% Tris-Glycine gels (Thermo Fisher). The electrophoresis was performed in 50 mM MOPS-50 mM Tris Base ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then pre-cleared for 1 hour by mixing 400uL sample with protein G Dynabeads (ThermoFisher Scientific, #10004D). Afterwards ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected using 1 μg/ ml of anti-rabbit Alexa Fluor 594 or anti-mouse Alexa Fluor 488 in 1%BSA as appropriate (Invitrogen). All antibodies were incubated at room temperature for 1 hr ...
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bioRxiv - Cancer Biology 2019Quote: ... or the lentiviral shDYRK2-1 or shDYRK2-2 together with the packaging vectors (psPAX2 and pMD2.G) and cultivated in OptiMEM medium (Invitrogen). The next day the cells were further grown in DMEM complete medium and one day later the lentivirus-containing supernatant was collected ...
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bioRxiv - Microbiology 2019Quote: ... the blot was probed with a 1:20 000 dilution of secondary antibody linked to alkaline phosphatase (ThermoFisher G-21060) in 5% skim milk-TBST for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: U2OS 2-6-3 cells containing the stably integrated lacO/tetO reporter gene cluster (Janicki et al., 2004) were grown in DMEM (1 g/l glucose, Gibco) without phenol-red supplemented with 10 % tetracycline-free fetal calf serum (FCS) ...
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bioRxiv - Neuroscience 2020Quote: ... Validation of shRNA constructs for reduction of Arl8b was done by transfecting mouse N2a cells at 40% confluency with pLKO3.G-Arl8b shRNA #1 and #2 either separately or together using Lipofectamine 2000 (Invitrogen). Transfection rates were > 80% and were ascertained by counts of GFP fluorescence on cell bodies ...
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bioRxiv - Cancer Biology 2020Quote: ... rinsed and incubated with secondary antibody Alexa Fluor 594 Goat Anti-mouse immunoglobulin G (IgG) (H/L) antibody (1:1000, Invitrogen) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Both cell lines were maintained in a plastic cultureware in low glucose (1 g/L) DMEM-GlutaMAX™ medium (Gibco) supplemented with 10 % foetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: B16-F1 cells and derived CapZa1/2 CRISPR#1 and CapZb KO#10 were cultured in DMEM (4.5 g/L glucose; Invitrogen, Germany), supplemented with 10% FCS (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2) at 55,000 × g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (ThermoFisher Scientific). The TB40r mGFP-IE-FKBP BAC was kindly provided by E ...
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bioRxiv - Microbiology 2021Quote: ... The gland/crypt-containing supernatant was washed by centrifugation at 400 x g for 2 minutes and the glands/crypts re-suspended in 1-2 ml advanced DMEM/F12 (12634-010; Gibco) containing 1X B27 supplement minus vitamin A (12587-010 ...
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bioRxiv - Neuroscience 2020Quote: ... The following day the slices were incubated with a primary monoclonal mouse antibody against Ankyrin G (1:100; Thermo Fisher Scientific Cat# 33-8800 ...
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bioRxiv - Cell Biology 2020Quote: ... per 1 mg of total protein were added along with 30 μL washed protein A/G UltraLink Resin (53133, ThermoFisher) and rotated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: The human lung epithelial carcinoma cell line A549 (ATCC CCL-185) was grown in DMEM 4.5 g ml−1 glucose (Life Technologies) with 10% foetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... cells were simultaneously incubated with secondary Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (Invitrogen, A-11001, 1:1000) and Streptavidin-Alexa Fluor 647 IgG (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The interaction between B56 subunits and PME-1 was detected by co-immunoprecipitation using anti-HA antibody immobilized on protein G magnetic beads (Invitrogen) to immunoprecipitate PME-1 bound to HA-B56 48 h after transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK293T (ATCC CRL-3216), was cultured in complete media (DMEM; 1 g/l glucose, 2 mM L-glutamine, 10 % heat-inactivated FBS (Gibco)) with 5 % CO2 at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg of pTM1-L and 1 μg of pCI-T7opt were re-suspended in 250 μL of Opti-MEM (Gibco). The plasmid cocktail was then gently mixed with 15 μL of TransIT LT1 transfection reagent (Mirus) ...