Labshake search
Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Slides were incubated overnight at 4° with primary antibodies diluted 1:200 in PBS (clone 302, ThermoFisher). LSAB and AEC Kits (DakoCytomation ...
-
bioRxiv - Genetics 2020Quote: ... 400 μl of lysate was treated with 4 μl of DNase I (1 U/μl, Thermo Scientific) and 8 μl of RNase I (100 U/μl ...
-
bioRxiv - Neuroscience 2022Quote: ... 4 dpf zebrafish larvae were exposed during 1 hour to LysoTracker Red DND-99 (Ref.L7528, Invitrogen/ThermoFisher) diluted at 5 μM in fish system water supplemented with methylene blue and PTU ...
-
bioRxiv - Neuroscience 2022Quote: ... 4 dpf zebrafish larvae were exposed during 1 hour to LysoTracker Red DND-99 (Ref.L7528, Invitrogen/ThermoFisher) diluted at 5 μM in fish system water supplemented with methylene blue and PTU ...
-
bioRxiv - Developmental Biology 2022Quote: ... 4 μl 10 μM dNTP mix and 1 μl RNase inhibitor (40 U/μl, Ambion, Cat# AM2682) was added to each tube containing RNAclean XP beads (input group ...
-
bioRxiv - Biochemistry 2022Quote: ... and maintained in Low Glucose DMEM (5.5 mM glucose, 1 mM pyruvate, 4 mM glutamine; #31600034, Gibco) supplemented with 10% fetal bovine serum (#12605729 ...
-
bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
-
bioRxiv - Immunology 2022Quote: THP-1 cells were differentiated into 4-well Nunc Lab-Tek II Chamber slides (ThermoFisher, Waltham, MA) at a density of 105 cells per well and differentiated as described above ...
-
bioRxiv - Microbiology 2022Quote: Spn serotype 4 strain TIGR4 was grown overnight on 1% agar- Todd Hewitt Broth (Thermo Fisher Scientific) with yeast (THY ...
-
bioRxiv - Microbiology 2024Quote: ... and incubated with primary antibodies overnight at 4 °C: pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:1000 dilution), STAT1 (Santa Cruz Biotechnology SC-417 ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
-
bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then stained using primary antibodies overnight at 4°C: tubulin (Invitrogen MA1-80017, 1:1000), aSyn (BD Transduction Laboratories 610786 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated at 4°C for 30 min with antibodies against CD45 (Invitrogen, #MCD4528, 1/25), CD31 (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... immortalized FSHD myoblasts were cultured in LHCN medium (4:1 DMEM:Medium 199 (ThermoFisher Scientific, Waltham, MA, USA) supplemented with 15% fetal bovine serum (FBS ...
-
bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
-
bioRxiv - Genetics 2023Quote: ... 1/10 of each eluate was resolved on a 4–12% NuPage Novex gel (Thermo Fisher Scientific) and stained with SilverQuest staining kit (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The samples were electroporated in the MES buffer in 1 mm 4%-12% Bis-Tris gels (Invitrogen) and blotted onto PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were probed with an SLC35A2 polyclonal antibody overnight at 4°C (1:2000, Invitrogen, PA5-77178), followed by incubation with goat anti-rabbit HRP-linked secondary antibody (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... washed (4 x 300 µL PBST) and blocked for 1h at RT with 1% BSA (Fisher Scientific) in PBS solution ...
-
bioRxiv - Bioengineering 2023Quote: ... constructs were fixed with 4% paraformaldehyde and washed with PBS and stained for LipidTox (Invitrogen 1:500) and DAPI (ThermoFisher ...
-
bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
-
bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
-
bioRxiv - Cancer Biology 2024Quote: ... Glut-1 and catalase proteins were separated on a 4% to 20% Tris-Glycine gel (Life Technologies) by SDS-PAGE and transferred to a nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
-
bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
-
bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
-
bioRxiv - Microbiology 2021Quote: ... mutations were inserted in fragment 8 (Extended Data Table 3) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, cat# A13312) according to the manufacturer’s protocol with the following primers ...
-
bioRxiv - Microbiology 2022Quote: ... The samples were sonicated and heated at 72°C for 10 minutes before loading 20 μl/well on NuPAGE™ 3 to 8% Tris-Acetate gels (ThermoFisher, EA03785BOX) alongside high molecular weight markers (ThermoFisher LC5699) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The resolved samples were run on either NuPAGE 3–8% Tris-acetate or NuPAGE 12% Bis-Tris protein gels (Thermo Fisher Scientific) with the appropriate running buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... and 40 μg of protein lysates were resolved on precast 3-8% gels in Tris-acetate buffer and then transferred to nitrocellulose membranes (all from Thermo Fisher Scientific). Membranes were blocked with 5% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved due to 3-8% Tris-acetate SDS-PAGE and transferred onto methanol-activated PVDF membrane with Pierce G2 Fast Blotter (Thermo Fisher Scientific). Afterwards ...
-
bioRxiv - Cancer Biology 2024Quote: ... + 100 mM beta-mercaptoethanol at 80 °C for 10 min and run on 3-8% Tris-acetate gels (Thermo Fisher Scientific, EA0375PK2). Gels were run at 80V for 3 h and transferred onto a PVDF membrane in Tris-Glycine buler for 90 min at 90V ...
-
bioRxiv - Molecular Biology 2024Quote: ... 25 – 50 ug of overall protein containing sample buffer and reducing buffer was loaded onto a 3 – 8% tris-acetate gel (Thermo Fisher Scientific) using tris acetate running buffer (Life Technologies) ...
-
bioRxiv - Cell Biology 2020Quote: ... and cultured at 35°C with 5% CO2 in medium containing 7.4 g/L MEM (ThermoFisher #11700-077) with the following supplements (in mM unless labeled otherwise) ...
-
bioRxiv - Cell Biology 2020Quote: ... Dy-Light 488-conjugated rat anti-mouse GPIbβ Ab (0.15µg/g;Emfret) and Alexa 647-conjugated fibrinogen (5% total fibrinogen; Invitrogen) were administered via a cannula inserted in the jugular vein ...
-
bioRxiv - Cell Biology 2022Quote: ... the IP sample was spun down at 13,000 r.p.m for 5 min and the supernatant was added to 60 μl of Dynabeads protein G (Invitrogen), previously equilibrated with IP buffer ...
-
bioRxiv - Genomics 2022Quote: ... The flow-through was centrifuged at 10,000 ×g for 5 min with a benchtop centrifuge (Thermo Fisher Scientific). After removing the supernatant ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were spun down at 500 g for 5 min washed with phosphate-buffered saline (PBS; Thermo Fisher, catalog no ...
-
bioRxiv - Systems Biology 2019Quote: ... followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl) ...
-
bioRxiv - Systems Biology 2019Quote: ... followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl) ...
-
bioRxiv - Systems Biology 2020Quote: ... we spun down the cells at 1,000 g for 5 min and resuspended in quenching buffer (DBPS, Gibco, 0.1% BSA ...
-
bioRxiv - Molecular Biology 2022Quote: ... All cells were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4.5 g/L) (Gibco 11965118) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... A total of 5 μg DNA was immunoprecipitated with preblocked protein A/G Dynabeads (Thermo Fisher Scientific, MA) complexed to 2.5 μg antibody (anti-CTCF ...
-
bioRxiv - Cell Biology 2022Quote: ... Isolated BM cells were maintained at 37 °C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat- inactivated fetal calf serum (FCS) ...
-
bioRxiv - Immunology 2024Quote: ... Isolated BM cells were maintained at 37°C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat-inactivated fetal calf serum (FCS) ...
-
bioRxiv - Plant Biology 2023Quote: ... samples were centrifuged at 13,000 × g for 5 min and loaded into SDS-PAGE gels (Invitrogen, Cat. NP0323BOX). Separated proteins were transferred using transfer stacks (Invitrogen ...
-
A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 5 g of tissue from brd-8V5 were purified with V5 antibody (Thermo Pierce)-conjugated Dynabeads (Life Technologies). A single protein band or the whole lane87 were cut off from the gel as indicated in the figure and used for mass spectrometry.