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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 × 2 minutes and blocked with SeaBlock blocking buffer (ThermoFisher) for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Interleukin-2 (IL-2; Clinigen, US-CA) was diluted in phosphate buffered saline (PBS; Thermo Fisher Scientific, US-CA) and added at a final concentration of 300 I.U./mL ...
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bioRxiv - Cell Biology 2024Quote: ... as previously described.26 The platelet pellet was resuspended in HEPES-buffered Tyrodes buffer with BSA 0.35% (w/v) containing 2 µM fura-2-AM (Invitrogen), and incubated for 45 minutes in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CM for 48 hours before loading with 2 μM fura-2 acetoxymethyl ester (Molecular Probes) in calcium imaging buffer (CIB ...
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bioRxiv - Physiology 2023Quote: ... Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester) acquired from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2023Quote: ... Two microliters of culture were spotted onto a 2 cm x 2 cm pad containing 1.5% UltraPure agarose (Invitrogen) + either BG-11 (for Se ...
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bioRxiv - Biochemistry 2023Quote: ... onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100 % humidity and 22°C ...
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bioRxiv - Neuroscience 2022Quote: [Ca2+]c was measured by ratiometric analysis using acetoxy-methyl-ester Fura-2 (Fura-2/AM; F1221 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... TG cultures were incubated with the calcium-sensitive dye Fura-2 AM (2 µM; Molecular Probes, Carlsbad, CA, USA) in Hank’s modified buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2; Gibco™, US-referred to as HMGS2 media) or Gibco™ Human Melanocyte Growth Supplement (HMGS ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated cells were incubated in media supplemented with 10 ng/mL of recombinant mouse IL-2 (rmIL-2) (ThermoFisher).
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked with 1x PBST containing 2% goat serum and 2 mg/ml BSA (Thermo Fisher Scientific, 37525), then incubated with 1/5000 Anti-Digoxigenin-AP (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... tertiary processing was used to enhance the strength of the punctate labeling in which a biotinylated secondary antibody (1:200) was followed by fluorescently conjugated streptavidin (SA-Alexa488, Invitrogen, 1-2 µg/ml).
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bioRxiv - Pathology 2021Quote: ... and proximal tubular cell line derived from normal kidney (HK-2, ATCC® CRL-2190™) were cultured with 1:1 DMEM/F12 (Gibco 11320-033) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Plasma and saliva IgAs were three-fold diluted (starting from 1:50 and 1:2 dilution, respectively) with infection media (DMEM medium (cat# 11995065, Thermo Fisher Scientific, Waltham, MA) containing 2% fetal bovine serum (FBS)) ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were incubated for 1 h at 22°C in the presence of a 2:1 mass ratio of TMT10plex reagent (Life Technologies cat. no. 90111) to peptides ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... neural induction was initiated by changing the culture medium to a 1:1 mixture of DMEM/N-2 (DMEM/F-12 + GlutaMAX (Life Technologies, cat# 31331-028); 1X N-2 (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 to 2 µg of StayGold mRNA was mixed with Plus™ reagent at a 1:1 ratio (µg:µL) in optiMEM (Thermo Fisher Scientific, Waltham, MA) to a final volume of 50 µL and incubated for 5 min at room temperature.
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bioRxiv - Neuroscience 2021Quote: ... Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific) for 45-60 min at 37 °C in standard extracellular solution ...
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bioRxiv - Molecular Biology 2020Quote: ... Acclaim PepMap 100 nanoViper C18 column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 100 Å) was used as pre-column for sample loading ...
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μl of RNase A/T1 mix (2 μg/μl, 5 U/μl, Thermo Scientific, cat#EN0551) was added to 400 μl of the cleared lysate and incubated for 20 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... blocked for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Calf Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... 5 x 106 S2 cells were seeded in 2 mL Schneider’s Drosophila media (Thermo Fisher, Waltham, Massachusetts) supplemented with 10% heat-inactivated fetal bovine serum in 9.6 cm2 wells of a tissue culture plate and incubated at 26 °C ...
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bioRxiv - Immunology 2020Quote: Cells were harvested from the peritoneum by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). After washing ...
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bioRxiv - Genetics 2020Quote: ... cells were passaged into differentiation media supplemented with 5-Ethynyl-2’-deoxyuridine (Life Technologies, A10044, 10 µM). On day 6 of differentiation ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
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bioRxiv - Neuroscience 2023Quote: Pregnant dams were injected intraperitoneally at e12.5 with a solution containing 5-Ethynyl-2’-deoxyuridine (EdU, ThermoFisher) and embryos were collected 2h after ...
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bioRxiv - Neuroscience 2023Quote: ... The BV-2 cells were cultured in DMEM supplemented with 5% heat-inactivated fetal bovine serum (Gibco) and 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... and the sample was washed with 1X PBS for 2 x 5 min using a Pipetman (Gibco), then blocked using the blocking solution for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of PCR products was run on a 2% E-gel EX Agarose Gel (ThermoFisher #G401002) for 10 minutes according to the settings programmed into the gel system ...
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Evolutionary Biology 2023Quote: We conducted all experiments using SM/5 media (Loomis and Sussman 1966) (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Cell Biology 2022Quote: ... then transfected for 5 hours with indicated plasmids in 2 mL Opti-MEM (Invitrogen, Cat. #31985-088) using Lipofectamine 3000 transfection kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: Dams were injected IP with 30 mg/kg body weight of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) 12 hours prior to harvesting at E18.5 ...
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bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (acetoxymethyl ester, #F1221; Thermo Fisher Scientific) with 0.01% pluronic F-127 (#P3000MP ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.3% Triton X-100 for 5 min and blocked in 2% blocking solution (Thermo fisher). Cells were incubated overnight with the primary antibodies washed in PBS and secondary antibodies were applied for 1 hr before counter-staining with DAPI ...
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bioRxiv - Synthetic Biology 2024Quote: EVs (2×109 particles in 15 µL) were mixed with a 5 µL sample buffer (NP0007, Invitrogen) and heated at 70 ℃ for 10 min ...
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bioRxiv - Cell Biology 2024Quote: The EU/EdU co-labelling mix was prepared by combining 100µM BrdU (5-Bromo-2’-deoxyuridine, Invitrogen) and 500µM EU in Nematostella medium with 2% DMSO and 50mM MgCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... and then loaded onto a trap column (PepMap100 C18, 300 μm × 2 mm, 5 μm; Thermo Scientific) followed by separation on an analytical column (PepMap100 C18 ...
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bioRxiv - Developmental Biology 2024Quote: ... We used Click-IT Plus 5-ethynyl-2′-deoxyuridine (EdU) AlexaFluor-555 or 488 (Thermo Fisher C10638) for all the Endoreplication labeling experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... 2% SDS and 5% glycerol supplemented with AEBSF and Halt protease and phosphatase inhibitors (Thermo Fisher Scientific). Protein concentrations were measured by BCA assay (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were again triple-washed with PBS and incubated in the appropriate secondary antibody (anti-rabbit-Alexa 594, 1:200, ThermoFisher, #A-11012, made in goat for Series 1; anti-goat-Alexa 594, 1:200, ThermoFisher, #A-11080, made in rabbit for Series 2) for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... hTSC were cultured in pre-coated 6-well plates (1 µg/ml Col IV) with 2 ml of EVT differentiation medium (EVTM: DMEM/F12, 0.1 mM 2-mercaptoethanol (Gibco 31350)) ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from HEK293T cell line 30 hours post transfection (HA-NRP1 and SARS-CoV-2-S1493-685 and or HA-NRP1-4Cys-4Ala and SARS-CoV-2-S1493-685) using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... B.1.617.2 Spikes were generated by overlapping PCR using a codon-optimized wild-type SARS-CoV-2 Spike gene (GeneArt, ThermoFisher) that was synthesized (Biobasic ...