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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 2 µL of Golden Gate reaction were added to 25 µL of 2× Phusion Polymerase Master Mix (ThermoFisher, Cat# F531L), mixed with 18 µL of H2O ...
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bioRxiv - Cell Biology 2024Quote: ... WEHI-231-GFP cells (2 x 105) were transiently transfected with 2 mM siRNA with the Neon Transfection Kit (MPK1025B, Invitrogen) using the Neon Electroporation system according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and digested in 10 mL of digestion buffer (DMEM/F12 supplemented with 2 mg/mL collagenase [Sigma, C5138], 2 mg/mL trypsin [Gibco, 27250018] ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was reverse crosslinked by incubation at 68°C for 2 h and purified by phenol:chloroform:isoamyl alcohol 25:24:1 (Life Technologies) extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... They were then maintained in RPE medium (70% DMEM, 30% Ham’s F12 nutrient mix, 2% B27-serum-free supplement, 1% antibiotic-antimycotic solution (Invitrogen)) for 2-3 months to form mature RPE monolayers ...
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bioRxiv - Cell Biology 2020Quote: ... the suspension was diluted 1:2 with fresh medium and passed through a 40 μm cell strainer (Fisher Scientific) to obtain single cell suspension ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (ARGi ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Nickase-1/2 constructs were individually transfected into CH12 via electroporation with Neon Transfection System (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... diluted (1:10,000) in pre-incubation (PI) buffer (PBS, 0.1% Tween 20, 2% sheep serum, 2mg/ml BSA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed with PBS followed by staining using streptavidin 488 (1:300, Jackson) diluted in PBS containing 0.4% Triton-X and 2% normal horse serum (NHS; Gibco) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... secondary Ab was incubated for 2 hours at room temperature (Alexa568-conjugated anti-mouse Ab, 1:400, Life Technologies). Brains were then mounted in Vectashield (Vector Labs ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (∼1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
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bioRxiv - Molecular Biology 2020Quote: The remaining RNA was combined into four final bsRNA-seq fractions (Figure 1) and 10μg RNA was spiked with ERCC Spike-in Mix 2 (Ambion) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... whole menisci were cut into small pieces (1-2 mm) and digested in DMEM (11996-065, Gibco, Carlsbad, CA) containing 2% penicillin/streptomycin (15140-122 ...
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bioRxiv - Genomics 2020Quote: ... Beads were handled before use with RNase OUT (40 U/μL; Life Technologies; 2 U enzyme/1 μL bead). The dRNA library was run on a R9.4 SpotON Flow Cell ...
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bioRxiv - Genomics 2020Quote: ... 2 µl cDNA (diluted 1:20) was used in a 6 µl Fast SYBR Green qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates ...
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bioRxiv - Biochemistry 2020Quote: ... using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Gel pieces were incubated in Elution buffer (1% formic acid, 2% acetonitrile in LC-MS grade water (Thermo Scientific)) and dried in a SpeedVac ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... RNA contents were isolated from the cells 1 or 2 hours after stimulation using TRIzol Reagent (Thermo Fisher Scientific) and reverse-transcribed using high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... A 2 μl aliquot of a 1:10 dilution of the ERCC RNA Spike-in Mix (Thermo Fisher Scientific) were added to each sample ...
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bioRxiv - Developmental Biology 2021Quote: Two BCAM-targeting siRNAs (siBCAM #1, siBCAM #2; Dharmacon) were transfected into CT29 hTSCs using Lipofectamine RNAiMAX (Thermo Fisher) at a concentration of 100 nM ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy3-labeled species-specific secondary antibodies for 2 h at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch). To avoid cross talk between multiple fluorophores ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibody Alexa 555 made in rabbit (Invitrogen, 1:500). After several washes ...
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bioRxiv - Systems Biology 2021Quote: We isolated pre-adipocyte cells from subcutaneous adipose tissue by dissecting tissue into small (1-2 mm) pieces with scissors and performing a 0.1% (w/v) collagenase II (Gibco) digestion in DMEM at 37oC for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then run on a Native PAGE gel and visualized with Alcian Blue (1:20 dilution of 1.25% stock solution in 2% acetic acid) followed by Silver Staining (ThermoFisher) following manufacturer protocols and imaged on the Bio-Rad Chemidoc MP Imaging System.
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bioRxiv - Microbiology 2021Quote: ... 2.5 mM probenecid were added to the cultures which were diluted 1:2 in DMEM with GlutaMAX (Gibco, Germany). 50 µl of the bacterial suspension were subsequently added to the screening plates which contained 1 µl of compound per well (concentrations are given in Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 and 3 dpf embryos were injected with 1–2 nL dextran tetramethyl rhodamine or dextran fluorescein (molecular weight 2,000 kDa, Invitrogen) at 10 mg/mL and imaged immediately ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells or Py8119 cells suspended at 1-2 million/150 µl of 33% (v/v) matrigel (Fisher Scientific) were transplanted subcutaneously to the right flank of 6-8 week-old BALB/c or C57BL/6J female mice ...
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bioRxiv - Cell Biology 2022Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (ThermoFisher); after 72 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... and then washed with 10% formamide in 2× SSC solution with Hoechst 33342 (1:1000, Thermo Fisher Scientific, H3570) and incubated at 37°C for 30 min in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µl of 2 µM Uni9 primer and 1µl of 10 mM dNTP (Thermo Scientific™, Cat no. # R0181) were mixed and warmed at 65 ºC for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Microbiology 2021Quote: ... bacteria were washed by centrifugation three times (11000 rcf for 2 minutes) and resuspended to OD 1.0 (~1 × 109 bacteria/ml) in RPMI (Gibco) + 0.05% human serum albumin (HSA ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with 2 pulses at 65 mV for 20 ms in a 1 mm cuvette (ThermoFisher, #15437270). After electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were 1-or 2-days post-transfection mounted in an AttoFluor cell chamber (A7816, Thermo Fisher Scientific) and microscopy medium (137 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue powder was transferred to 2 mL microcentrifuge tube and 1 mL TRIzol Reagent (ThermoFisher, Cat no 15596018) was added ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were split 1-2 times per week after dissociation with EDTA in DPBS (final concentration 0.5mM) (Gibco, 12605010). Media was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Immunology 2022Quote: ... 1.5×106 CD45.1 cells were incubated with 2×106 Balb/c splenocytes in the presence of 1 mg/mL GolgiStop (Invitrogen) for 4-5 h at 37 C ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using a 2:1 ratio of random primers to Oligo(dT) with SuperScript IV RT (Invitrogen). qPCR was performed with PerfeCTa SYBR Green FastMix for iQ (Quanta Biosciences ...