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Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 10% v:v Knockout Serum Replacement [Gibco], 1% v:v 100x Non-essential Amino Acids [Gibco], 2% v:v 50X B27 Supplement [Gibco] ...
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bioRxiv - Molecular Biology 2024Quote: ... as published.62 Nuclei was visualized by 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, from ThermoFisher) as published.23
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Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damagebioRxiv - Microbiology 2024Quote: ... Sections were incubated overnight at 4℃ with primary antibodies specific for SARS-CoV-2 Nucleocapsid (1:300, Invitrogen), Spike (1:500 ...
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bioRxiv - Immunology 2024Quote: ... Tconv cells (2 × 104 cells/well) were stained with the fluorescent dye CFSE at 1 μg/ml (Invitrogen). Flow cytometry analyzing CFSE dilution was performed by gating on CFSE+ cells stimulated for 72 hours in round- bottomed 96-well plates (Corning Falcon ...
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bioRxiv - Immunology 2024Quote: ... genes encoding MD39 HIV Env gp140 were cloned into pHLsec by Genscript and co-transfected with human furin in a pcDNA3.1 plasmid using a 2:1 trimer:furin DNA ratio with polyethylenimine into FreeStyle 293-F cells (ThermoFisher) and incubated for 6 days ...
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bioRxiv - Immunology 2024Quote: 1-2 mL plasma from each volunteer was purified with Protein G Plus agarose affinity chromatography (ThermoFisher Scientific), and IgG F(ab′)2 fragments were generated with IdeS at a 1:50 ratio at 37°C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1 bead:2 Tresp), in RPMI 1640 medium (Nacalai Tesque ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated for 1 hour in secondary antibodies with 0.5 μg/ml 4,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Antibodies used are listed in Table S6 ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were rinsed and incubated with 4,6-diamino-2-phenyindole dilactate (DAPI, 1:1000, Molecular Probes, R415) for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... 100 nM) were loaded on 2 % agarose gel containing 1 × fluorescent dye SYBR Safe DNA Gel Stain (Invitrogen), run at 150 V for 45 minutes ...
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bioRxiv - Genetics 2024Quote: ... tube 2 (well specific tube, 2A, 2B, etc.) contained 1 µl of P3000 enhancer (Thermo Fisher, REF-L3000008), 1 µg/µl of plasmid DNA ...
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bioRxiv - Physiology 2024Quote: ... to identify cytoplasmic F-actin fragments and 4’,6-diamidino-2-phenylidole (DAPI) (Invitrogen Cat. #D1306; 1:1,000) to label nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... A488 rabbit anti-GFP (1:1000, for 2 hours at room temperature, Molecular Probes Cat# A-21311, RRID:AB_221477). Tissues were washed thrice in 1X PBS and incubated at room temperature for 2 hours in the appropriate secondary antibodies (1:1000 in 5% NGS in 0.25% PBST) ...
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bioRxiv - Bioengineering 2024Quote: ... media was replaced with fresh media containing 1 μg/mL doxycycline and 2 μg/mL puromycin (Gibco, A1113803). Five days post-nucleofection ...
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bioRxiv - Biophysics 2024Quote: ... 2 µL per sample were desalted using a ProSwift™ RP-4H 1 ×50mm column (Thermo Fisher Scientific) followed by injection into a maXis II ETD ESI LCMS (Bruker Daltonics) ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Undeveloped photoresist was washed off with SU-8 developer (1-methoxy-2-propanol acetate, Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... and 1:400 Alexa Fluor 488 F (ab′)2 goat anti−mouse IgG (Thermo Fisher Scientific, A-11017). These slides were washed with 1X PBS and mounted with Vectashield plus antifade mounting medium (Biolynx ...
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bioRxiv - Cell Biology 2024Quote: ... 2% SDS lysis buffer using tip-probe sonication (10 X 0.5s pulses; Level 1) (Fisher Scientific, Waltham, MA). Protein was quantified by 660 nm spectrometry using detergent-compatible reagents ...
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bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 dpf were injected with 1–2 nL of dextran tetramethyl rhodamine (molecular weight 2000 kDa, Invitrogen) at a concentration of 10 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with 1 μg of plasmid DNA mixed with 2 μL of lipofectamine 2000 (Invitrogen #11668019) on 37 °C for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 2 h with Alexa Fluor 488 donkey anti-goat IgG (Invitrogen, 1:400, #A11055), Alexa Fluor 568 donkey anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and then blocked for 2 h with PBS-Tween (PBST) containing 2% (v/v) goat serum (Gibco, MD, USA). Three-fold serial dilutions of serum (starting at 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... was cultured at 2-8×105 cells/ml in Fischer’s medium with 20% horse serum and 2 mM L-glutamine (all Gibco).
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bioRxiv - Molecular Biology 2020Quote: ... proteins were dialysed in PBS for 2 x 2 hours using Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher). Protein aliquots were frozen and stored at −80°C until use.
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bioRxiv - Immunology 2022Quote: Purified IL-2 expanded MAIT cells (MAITIL-2) were stimulated for 18 hours with anti-CD3/CD28 Dynabeads (Thermofisher), IL-12 (50ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fura-2-acetoxymethyl ester (Fura-2-AM) and Pluronic F-127 were purchased from Molecular Probes (Eugene, OR, USA) and dissolved in dimethylsulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2021Quote: 2 mg of 0-2 hr embryos were harvested and RNA was extracted with TRIzol Reagent (#15596026, ThermoFisher Scientific). The PAT test was conducted following the manufacturer’s protocol (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... SALK_015201 and irr-2 genomic DNA using gene specific primers (Supplemental Table 2) with Phusion polymerase (Thermo Fisher Scientific) and cleaned up using the Gene-JET PCR Purification Kit from Thermofisher (catalog number K0701) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 HEK293T cells were transfected with a 2:1:2 mass ratio of psPAX.2:pMD2.G:pLentiCMV/TO PuroDEST pUL21 (815 ng total DNA) using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... embryos were collected and brains were removed in ice-cooled Ca+2/Mg+2-free Hank’s balanced salt solution (Gibco) with 10mM HEPES (Thermofisher) ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Immunology 2022Quote: ... GATA-2-expressing vector was constructed by cloning synthesized mouse GATA-2 sequences into eukaryotic expression vector pcDNA3.1 (+) (Invitrogen) with restriction enzymes of NheI and EcoRI (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... both somal and axonal compartments were incubated with 2 μM of the calcium indicator dye Fura-2-AM (Invitrogen) in imaging buffer consisting of HBSS [Ca2+ and Mg2+-free] supplemented with 10 mM HEPES (ThermoFischer) ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
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bioRxiv - Microbiology 2021Quote: Vero cells were seeded at 1.0 × 104cells per well with Dulbecco’s modified Eagle’s medium (DMEM; Welgene) supplemented with 2% heat-inactivated fetal bovine serum (FBS) and 2% antibiotic-antimycotic solution (Gibco) in a black ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco), and dissociated by 8–10 bouts of trituration ...
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bioRxiv - Cell Biology 2021Quote: ... and 2+2 μg of plasmid DNA encoding the proteins were mixed in Opti-MEM™ (Thermo Fisher Scientific) and applied onto the cells in a single well ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... the cell pellets were incubated for an hour in 2 uM 2’,7’-dichlorodihydrogen-fluorescein diacetate (H2DCF-DA, Invitrogen) in phenol red free media (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... counter stained with 2 mg/mL Alexa Fluor® 594 F(ab’)2 goat anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... the dorsal cord was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco) and dissociated into single cells by 8–10 bouts of trituration ...
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bioRxiv - Microbiology 2020Quote: ... were seeded with 3.0 x 105 /ml Vero E6 cells in 0.5 ml volumes of 2 x MEM medium containing Glutamax (2 x MEM Gibco) + 10% FCS (Sigma, ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cultures were concentrated roughly four-fold by centrifugation (2 min 2000 xg) and 2 μl cells were placed on a slice of 1.5% UltraPure agarose (Invitrogen) dissolved in minimal media ...
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bioRxiv - Neuroscience 2023Quote: ... the animal’s brain was extracted and the meninges removed in a Petri dish containing cooled HBSS +/+ (Hank’s Balanced Salt Solution with Ca 2+ and Mg 2+: Gibco) to maintain their metabolism ...
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bioRxiv - Physiology 2022Quote: Constructs for F4-2_Fab (hereafter F4-2) and L6-F4-2 were cloned into the pcDNA3.1(+) mammalian expression vector (Thermo Fisher). The F4-2 heavy chain contains a 6xHis tag on its C-terminus ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs-derived neuronal cells were incubated with the cell-permeant Fura-2 acetoxylmethylester (2 μM; Molecular probes, Life technologies) for 1 h at 37 °C in culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs-derived neuronal cells were incubated with the cell-permeant Fura-2 acetoxylmethylester (2 μM; Molecular probes, Life technologies) for 1 h at 37 °C in culture medium ...