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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Total RNAs were extracted from N2 and daf-2(e1370) worms on adult day 1 using TRIzol (INVITROGEN), followed by using DNase I to remove contaminant DNA ...
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bioRxiv - Cell Biology 2021Quote: ... for 1–2 h at 37°C followed by a 10 min incubation with Accutase (Thermo Fisher Scientific) at 37°C to obtain a single cell suspension ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h with donkey anti-rabbit Alexa Fluor488 (1:200, ThermoFisher Scientific, A-21206) in 1% FBS PBS-Tx ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated for 2 hours at RT with streptavidin Alexa488 conjugate (S11223, Molecular Probes, dilution 1:500). Washings ...
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bioRxiv - Microbiology 2021Quote: ... As secondary Ab were used: goat anti-mouse F(ab)’2 AlexaFluor 488 (Molecular Probes, A11017, 1:300) and goat anti-rabbit Cy3 (Jackson Laboratories 111-166-003 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The electroporated cells were immediately transferred to 2 mL microfuge tubes with 1 mL of SOC media (Invitrogen), grown at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% Triton X-100) containing fresh protease inhibitors (0.1% 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride (PMSF; ThermoFisher Scientific), 1 mM benzamidine hydrochloride ...
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bioRxiv - Cell Biology 2021Quote: ... the tissues were mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded with 2×105 density in a 1 mL medium in 12-well plates (ThermoFisher Scientific). After 24-hour incubation ...
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bioRxiv - Immunology 2022Quote: ... Total cDNA was generated from 1-2 µg of RNA using random hexamer primers and Revertaid RT (Thermofisher). Taqman qPCR was used to quantify LT mRNA levels with normalization to TATA-Box Binding protein and compared to a standard curve to determine copy number (Maru et al. ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Hybridization mix contained hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1□mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Microbiology 2022Quote: The Spike gene sequence from the severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) was codon optimized (GeneArt, ThermoFisher) and gene blocks with overlapping sequences were synthesized by Bio Basic Inc (Markham ...
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bioRxiv - Microbiology 2022Quote: ... Fifty μL of 1:3000 diluted mouse anti-SARS-CoV-2 Spike Protein S1 monoclonal antibody (GT623, Invitrogen) in 1% BSA-PBS blocking buffer was added and incubated at room temperature for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours with Alexa fluor™ 488 goat anti-rabbit (1:1000, Invitrogen), Alexa fluor™ 647 anti-rat (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1 mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Molecular Biology 2020Quote: ... LIVE/DEAD discrimination was performed by staining with 1 µg/ml of 4’6-diamidino-2-phenylindole (DAPI, Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... 100μl of 2% milk prepared in 0.1% PBST containing 1:1000 concentration of mouse αGFP antibody (Life Technologies #A-11120 ...
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bioRxiv - Neuroscience 2021Quote: ... human DRGs immediately were cut into 1-2 mm pieces and stored in RNA-later (ThermoFisher, Cat# AM7021). For ISH ...
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bioRxiv - Microbiology 2020Quote: ... Parasite nuclei were visualized by incubating samples with 1-2 μg/ml Hoechst 33342 (Thermo Scientific Pierce 62249) for 10-20 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were incubated for 2 hours in secondary antibody [Goat-anti-rabbit (1:3000; Alexafluor 568, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were transfected with PB-TO-hNIL vector in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (Invitrogen), then selected after 24-48 hours for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted from infiltrated leaf patches in 1 ml 2×NuPAGE LDS sample buffer (Invitrogen, NP0008) containing 0.05mL/mL β-mercaptoethanol ...
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bioRxiv - Physiology 2021Quote: ... Complementary DNA was synthesized from 1 to 2 μg of RNA using SuperScript VILO Master Mix (Life Technologies). Quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... replicon transfected PS cells were maintained medium supplemented with in a 2% FBS and 1% Penicillin-Streptomycin (Invitrogen). Sf9 cells (ATCC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... 1-2 µg total RNA was reverse transcribed using SuperScript IV VILO Master Mix with ezDNase Enzyme (Invitrogen) into cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were rinsed 2 x in DI water before mounting onto 1 mm-thick glass slides (Thermo Scientific) using anti-fade mounting medium (Dako).
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bioRxiv - Cell Biology 2021Quote: ... 1:1000) for 2 hrs and then washed 3X in PBS before mounting with Prolong Diamond (Life Technologies).
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bioRxiv - Physiology 2020Quote: ... DAPI (4’,6-Diamidino-2-Phenylindole Dihydrochloride; 1:2000 diluted in PBTD; Thermo Fisher Scientific, Stockholm, Sweden; 62248) was added for 3 minutes followed by washing in PBTD for 25 minutes and mounting with ProLong gold antifade reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: The scrambled siRNA or the specific VEGFR siRNA (VEGFR-1-VEGFR-2 siRNA, Ambion Life Technologies, Monza, Italy) were intrathecally (i.t. ...
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bioRxiv - Neuroscience 2021Quote: The scrambled siRNA or the specific VEGFR siRNA (VEGFR-1-VEGFR-2 siRNA, Ambion Life Technologies, Monza, Italy) were intrathecally (i.t. ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended (2.25 x 107cells/1.5mL) in DMEM/F12 supplemented with 1% penicillin-streptomycin and 2% B27 (all from ThermoFisher) and transduced overnight with a TdTomato-lentivirus in a 2ml tube using an Intelli-Mixer RM-2L (Dutcher) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 hours of incubation in blocking solution containing anti-chicken Alexafluor488 (1:400; Life Technologies A11039) and anti-goat Alexafluor594 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... then split for 1) propagation and 2) screening by Sanger sequencing using a 3730xl DNA Analyzer (Applied Biosystems). Colonies positive for the target edit and without other changes in the immediate vicinity (within 300-400 bp ...
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bioRxiv - Neuroscience 2021Quote: ... slices were counterstained with 4’,6-diamidine-2’-phenylindole 502 dihydrochloride (DAPI, D3571, Molecular Probes; dilution 1:1000) for 5 minutes followed by 2 minutes wash in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... and diluted to 1-2 million cells/mL using a Countess™ Automated cell counter (Invitrogen; Carlsbad, CA). On each day of experiments ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (RBD, 1:1,000, Invitrogen cat. no. PA5-114451). All primary antibodies were diluted in PBST + 5% milk and applied to membranes for ≥16 hours at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Treated serum was then serially diluted 1 in 2 with PBS in 96 well v-bottom plates (Nunc) in 50 μL volumes ...
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bioRxiv - Immunology 2020Quote: ... and lymphocytes were resuspended in complete RPMI (cRPMI- RPMI + 10% FCS + 2% Penicillin-Streptomycin + 1% L-glutamine (Gibco)) ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... incubating for 2 hrs at ambient temperature with Alexa Fluor donkey anti-Rabbit 555 1:200 (A31572, Invitrogen), DAPI for 5 minutes and then cover slipping the tissue sections with Aqua-Poly mount (Polysciences) ...
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bioRxiv - Microbiology 2023Quote: ... parasite nuclei were visualized by incubating samples with 1-2 μg/mL Hoechst 33342 (Thermo Scientific Pierce 62249) for 10-20 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...