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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (~1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA (1-2 μg) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR was carried out in the BioRad CFX96 Real-Time System using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... VH and VL plasmids were mixed at 1:2 ratio and were transfected into Expi293F cells (Thermo Fisher Scientific), which were cultured at 37 °C and 8% CO2 for 5 days ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... RNA was treated 1 h at 37 °C with 2 units of Turbo DNAse (ThermoFisher Scientific, Waltham, MA, USA), purified using NucleoSpin RNA Clean-up (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... followed by 2 h staining with secondary donkey anti-mouse Alexa Fluor 546 antibody (Invitrogen, A10036, 1:500 dilution) and 1μg/ml DAPI.
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bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...
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bioRxiv - Immunology 2021Quote: ... The sections were then incubated for 2 hours at room temperature using: rabbit polyclonal antibody-1 for myeloperoxidase (Invitrogen) to examine neutrophils ...
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bioRxiv - Bioengineering 2022Quote: ... Half of the medium was replaced by pre-warmed PBS containing 2 μL Hoechst (1 mg/mL Thermofisher Scientific). After 30 min incubation ...
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bioRxiv - Biophysics 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit Alexa 647, Invitrogen, A-27040 ...
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bioRxiv - Cancer Biology 2022Quote: ... thinly sliced (1-2 mm) tissue samples were fixed overnight at 4°C in neutral-buffered formalin (Fisher Scientific) with PhosSTOP added (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... the mice were perfused for 1 min to 2 min with Hank’s balanced salt solution (14025-050; ThermoFisher Scientific), followed by 5 min perfusion with 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2022Quote: ... was transfected into Lenti-x 293T cells with 2:1 (psPAX2/pMD2G) plasmids using Lipofectamine 2000 (Thermo Fisher # 12566014) at a ratio of 1:1 (Lipofectamine 2000/nucleic acid (μg) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and plated onto coverslips (1:2 split) or Nunc 8 well chamber slides (Thermo Fisher # 155409PK) (20-40k cells per well).
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Human THP-1 cells were cultured in RPMI-1640 medium containing 2 mM L-glutamine (Gibco; Grand Island, NY), 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila Schneider 2 (S2) lines were maintained in 1 X Schneider’s medium supplemented with 10% heat inactivated fetal bovine serum (Gibco) and 50 mg/ml gentamicin antibiotic (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 μl NET-2 and extracted with an equal volume of phenol:chloroform:isoamyl 25:24:1 (Invitrogen 15593031). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with the Ca2+ Calibration Kit #1 (Invitrogen, Waltham, USA).
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bioRxiv - Cell Biology 2022Quote: ... The PAA gel surface was then incubated with a solution of 2 mg ml−1 Sulpho-SANPAH (4822589, ThermoFisher) under ultraviolet light for 5 min (wavelength of 365 nm at 5 cm distance) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM 2-mercaptoethanol and 10 mM imidazole) and then loaded onto a HisTrap HP column (ThermoFisher, Waltham, USA). The eluted fractions containing FHS-OGOX1 were pooled and dialyzed in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 1 μg of RNA per sample (10.6 µL) was incubated with 2 µL TURBO DNase (Thermo Fisher Scientific) and 1.4 µL TURBO DNase buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... cells were dissociated and plated (1:2 ratio) at high density and maintained in maturation media (Neurobasal media (Gibco) + B27 supplement (Gibco ...
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bioRxiv - Plant Biology 2024Quote: DAPI (4′,6-diamidino-2-phenylindole) solution: Dilute 1 mg/ml DAPI (ThermoFisher, Cat. No. 62248, Rockford, IL, USA) to a final concentration of 0.5 µg/ml in 1x PBST (0.1% Tween-20).
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bioRxiv - Microbiology 2023Quote: ... sonicated in 1-2% SDS at 37 kHz and 100% power in a Fisherbrand™ bath sonicator (Fisher Scientific) for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were then kept at 28.5°C with fresh E3 medium supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics). Around 3-4 hours after heat-shock ...
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bioRxiv - Developmental Biology 2024Quote: R-follicles were incubated for 1 hour in Maintenance Media containing freshly prepared 2 µM Calcein-AM (C3100MP, Invitrogen). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in goat anti-rabbit Alexa Fluor 568 secondary antibody (1:200 in 2% BSA and 0.1% Triton; Invitrogen) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The Dynabeads were washed three times with 1 ml of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific), with each wash performed after 1 minute of incubation ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with serum free RPMI 1640 medium and incubated with 1 μM Rhod-2-AM (Invitrogen, R1244) together with antibodies for CD4 (GK1.5 ...
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bioRxiv - Cell Biology 2024Quote: ... for immunofluorescence analysis or were lysed in IGEPAL-C360 lysis buffer (20 mM Tris HCl pH 8.1, 37 mM NaCl, 1% IGEPAL-C360, 10% glycerol, 2 mM EDTA supplemented with protease inhibitor cocktail from ThermoFisher) for immunoblotting.
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with 4’,6-diamidino-2-phenylindole (DAPI; D1306, Thermo Fisher Scientific; 1:1000 in PBS) for 2min at room temperature to counterstain the nucleus before being washed twice in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... About 1-2 μg indicated plasmid was used according to the instruction of lipofectamine 3000 (Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2024Quote: ... activated and unactivated iPSC-Tregs were co-cultured with activated (2:1 beads:cells) Tconv labeled with Cell Trace Violet (Thermofisher) in X-VIVO15 media supplemented with pen/strep ...
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bioRxiv - Cell Biology 2024Quote: ... and RT was performed with 1-2 μg RNA using SuperScript™ IV VILO™ Mastermix (11756050, Thermo Fisher) with the following conditions ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 LipidTOX was added along with 0.25 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: Slides were rehydrated with dPBS for 2 minutes before incubation in 1:500 wisteria floribunda agglutinin (Invitrogen, Waltham, MA) overnight at room temperature in the dark in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% (v/v) sodium deoxycholate) supplemented with 1% protease inhibitor cocktail and 2% RiboLock RNase Inhibitor (#EO0381, Thermo Scientific). The RNA was sheared ...
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bioRxiv - Cancer Biology 2022Quote: ... were incubated with 100 μl of 2 mg ml-1 p-NPP (p-nitrophenyl phosphate) substrate (#34045; Thermo Fisher) diluted in AP-buffer ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNAs were synthesized from total RNA (1 μg) for each sample using SuperScript 2 reverse transcriptase (18064014, Thermo Fisher), diluted ten times with water ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were stained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, 1:1000, Invitrogen, Thermo Fisher Scientific, MA) for 10 mins ...
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bioRxiv - Molecular Biology 2023Quote: 1×105 HeLaK cells expressing GFP or GFP-ZNF512B or treated with Doxorubicin (100 nM, 2 days, Fisher Scientific) were seeded on coverslips in 24-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were stained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, 1:1000, Invitrogen, Thermo Fisher Scientific, MA) for 10 mins ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...