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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Doxycycline (2 g/L, Fisher Scientific, AC446060050) was added on day 0 to induce TetO gene expression and retained in the medium until the end of the experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 2 mM glutamine (Gibco, 25030081), 10% FBS (Avantor Seradigm ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% N-2 supplement (Gibco, Life Technologies), 50 μg/ml bovine serum albumin (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% N-2 supplement (Gibco, Life Technologies), 50 μg/ml bovine serum albumin (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 μg/ml of puromycin (Gibco), depending on the resistance marker present in the lentiviral expression vector ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2 mM L-glutamine (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% MEM amino acid solutions (Gibco, 11140050) and 1mM Sodium pyruvate (Sigma ...
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bioRxiv - Immunology 2024Quote: ... using the iBlot 2 Transfer System (Invitrogen). We blocked the membrane for 1 hour with Intercept Blocking Buffer (LiCor ...
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bioRxiv - Genetics 2024Quote: ... 2 μl of 25mM MgCl2 (Applied Biosystems), 0.4 μl of 20 mg/ml BSA (BioLabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2% Matrigel (CB-40234, Thermo Fisher) (Okae et al ...
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bioRxiv - Developmental Biology 2024Quote: ... FluoZin-3AM (2 µM; F24195, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... was done using the iBlot 2 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 2 nM L-glutamine (Gibco), 10 μg/ml insulin (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 µM 2-Mercaptoethanol (Gibco 31350-010),10 ng/mL Leukemia Inhibitory Factor (LIF ...
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bioRxiv - Cell Biology 2024Quote: ... and 2% B27 (Thermo Fisher, cat. 17504044) (from here on referred to as supplemented NB) ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.3 with 2 parts RPMI (Invitrogen) supplemented with 20% FCS (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
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bioRxiv - Cell Biology 2021Quote: SK-MEL-2 cells from clone Ti13 (hCLTAEN-1 /hDNM2EN-1) were cultured in DMEM/F12 with GlutaMax™ supplement (10565-018, Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... We cultured the mouse embryonic stem cell (ESC) line in 2-inhibitor based medium (Advanced DMEM/F12:Neurobasal (1:1) medium (Thermo Fisher, 12634028, 10888022) supplemented with 2.5% ESC-grade Tet-negative fetal bovine serum (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488, Goat-anti-chicken, Invitrogen A11039, 1:500; Alexa Fluor 594 goat anti-rabbit, Invitrogen A11012, 1:500). A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: Expanded Tregs or Tconvs were activated with IL-2 (100 IU/mL) and 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads T-Expander) for 4 d ...
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bioRxiv - Biophysics 2023Quote: ... cells were resuspended in the appropriate amount of maintaining medium (RPMI Gibco® supplemented with 10% FBS, 2 mM GlutaMAX Sigma®, 1% Penicillin/Streptomycin Sigma®, 1 mM Sodium Pyruvate Gibco) and plated to allow maintenance and amplification ...
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bioRxiv - Genomics 2023Quote: ... ESC were adapted for > 2 weeks to N2B27 serum-free medium consisting of 1:1 mix of neurobasal medium (Gibco, Cat. No 1103049) and DMEM/F12 (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... for 2 days and Neurobasal/B27 medium (Neurobasal Medium, Thermo Fisher Scientific; supplemented with 1× B27, Thermo Fisher Scientific; 1× Glutamax, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: Gravid adult worms were dissected in 1x M9 with a 22G x 1-1/2” needles (Fisher Scientific catalog number 14-840-91) to release embryos ...
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bioRxiv - Immunology 2023Quote: ... fresh media was supplemented with 30 U/mL of IL-2 and a 1:1 ratio of anti-CD3 and -CD28 Dynabeads (Thermo Fisher Cat# 11131D) and similarly treated with aphidicolin ...
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bioRxiv - Neuroscience 2023Quote: ... and maintained in N2B27 media (1:1 of DMEM F12 Glutamax [Thermo Fisher Scientific, 10565018] and Neurobasal media [Thermo Fisher Scientific, 12348017], 1X N-2 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Human endothelial cells in muscles were stained with biotinylated Ulex Europaeus Agglutinin I Lectin (UEA I, B-1065-2, Vectorlab, 1:100 dilution) and FITC-streptavidin (11-4317-87, Invitrogen, 1:250 dilution). Nuclei was stained with DAPI ...
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bioRxiv - Cell Biology 2024Quote: For nuclei and F-actin staining were visualized with 4’,6-diamidino-2-phenylindole (DAPI) (#D523, Dojindo; 1:1000 dilution) and phalloidin Alexa Fluor plus 405-conjugated (#A30104, Invitrogen; 1:400 dilution), phalloidin ATTO 565-conjugated (#94072 ...
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bioRxiv - Genomics 2024Quote: ... This was done by resuspending the cells in 1 mL of 1 X PBS and cross-linked in 2% formaldehyde (Thermo Fisher Scientific, 28908) by inverting the tube 10 times before incubating at RT for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... the media was replaced with 100 µL of fresh 2:1 mixed media supplemented with 100 ng mL−1 recombinant human vascular endothelial growth factor (Thermo Fisher Scientific PHC9394). The samples were cultured for another 2 days before fixation with 40 mg mL−1 formaldehyde in PBS for 40 min and then washed 3 times with PBS ...
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bioRxiv - Physiology 2023Quote: ... followed by 2h incubation in goat anti-chicken 488 (1:400; Molecular Probes) and biotinylated goat anti-rabbit immunoglobulins (1:400 ...
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bioRxiv - Immunology 2022Quote: ... slides were incubated for 2h at room temperature with DAPI (1:1000, Invitrogen) and appropriate secondary antibodies (goat anti Armenian Hamster AF647 and AF555 (1:500 ...
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bioRxiv - Pathology 2021Quote: ... Larvae were injected in the common cardinal vein with 1 nl of Cy3-β-Lactoglobulin (5 mg/ml) and/or Alexa fluor™ 488 10 kDa dextran (D22910, Thermo Fisher, at 2 mg/ml) diluted in sterile H2O and successful injections were screened for on a Leica Mz10F fluorescent stereomicroscope ...
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bioRxiv - Microbiology 2023Quote: ... The TMT10-plex-tagged sample (1 µg) was loaded onto an Acclaim PepMap100 C18 trap column (Thermo Fisher Scientific, 100 µm × 2 cm, 100 Å, 5 µm) at 5 µL/min using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Star635P anti-guinea pig (Abberior, cat. 2-0112-007-1, 1:100 dilution) and Alexa Fluor 594 anti-rabbit (Thermo Fisher, cat. A-21207, 1:100 dilution), Alexa Fluor 488 anti-chicken (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were spiked-in with the ERCC spike-in controls (2 μL/sample, diluted 1:100, Invitrogen #4456740), treated with on-column DNase digestion according to manufacturer’s specifications (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... supernatant was removed and cells were incubated with 1 μg of Fura-2 AM dye (Thermo Scientific, USA) at 1mg/ml DMSO in a total volume of 100µl for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... and probed for 2 h with an FITC-conjugated goat anti-rat antibody (1:300, Thermo Fisher Scientific). VE-cadherin in the superficial vascular plexus and deep capillary plexus was visualized in the retinas by confocal microscopy as illustrated in Fig ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were expressed in 1-2 liters of SF900 III SFM media (Thermo Fisher Scientific, Waltham MA) set at 7 × 105 cells/ml in 2.8L or 5L Optimum Growth Flasks (Thomson Instrument Company Oceanside ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... 2% vol/vol FBS and 1% vol/vol antibiotic-antimycotic (#15240062, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) referred to hereafter as HBSS+ ...
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bioRxiv - Bioengineering 2020Quote: Panc-1 and MiaPaCa-2 cells were homogenized in a radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher Scientific, #89901) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Parasite nuclei were visualized by incubating samples with 1-2 µg/ml Hoechst 33342 (Thermo Scientific Pierce 62249) for 10-20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... at 37°C for 1 minute and further dissociated in digestion medium containing 2 mg/ml Dispase (Invitrogen) and 0.1 mg/ml DNase I (Worthington ...