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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 4 μL of sample was applied and the grid was blotted for 5 seconds at 4 °C and 95% relative humidity then plunge-frozen with liquid ethane using a Vitrobot (ThermoFisher). Samples were clipped and screened by Glacios transmission electron microscope before 5,276 movies were collected on a Titan Krios transmission electron microscope at the Center for Electron Microscopy and Analysis (CEMAS ...
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bioRxiv - Molecular Biology 2024Quote: ... Villi were collected by centrifugation at 300 g for 5 min and single-cell suspension was generated by rotating in 20ml of 4% TrypLE solution (Invitrogen) in DMEM at 37°C for 30 min ...
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bioRxiv - Genetics 2024Quote: ... Samples were boiled at 95°C for 5 min before loading (60 µg) on 4-12% Bis-Tris gel (ThermoFisher) and ran with 1X MES buffer (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... filtered through a 5 µm strainer (04-004-2323, Sysmex) and washed with 4 ml of ice cold 0,01% BSA (AM2616, Thermo Fisher) in DPBS (59331C ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged every 4-5 days up to 70-85% confluency utilizing 0.5 mM EDTA (Thermo Fisher Scientific). On the first day post-subculturing ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Microbiology 2023Quote: ... Matched high and low molecular weight fractions adjusted to supernatant concentration equivalents in 0.9% saline were measured for fluorescence before and after addition (15 min incubation) of 5 μg/mL FM 4-64 (Invitrogen), and the background reading subtracted to obtain the final measurement.
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated by electrophoresis for a short amount of time (5 min) at 200 V on a NuPAGE 4-12% Bis-Tris gel in 1X MES/SDS (Invitrogen) running buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum) for 1 hr at 4°C and then with a primary antibody anti-GFP (Invitrogen, catalog # G10362 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were suspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged (13,000 × g for 5 min at 4°C) and supernatants are precleared with 10 μl protein G beads (Invitrogen; 1004D) at 4°C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated at 95°C for 5 minutes before loading on a 4-12% gradient Bis-Tris gel (Invitrogen). MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged at 500 g for 5 minutes at 4°C and red blood cells were hemolyzed using ACK buffer (Gibco). White blood cells were then incubated with 1:1000 Fc Block (AbLab ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... blocked in 5% donkey serum and incubated overnight at 4°C with a rabbit anti-Rbm20 antibody (PA5-53068, Invitrogen) at 0.5 μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 5 min prior to resolving (40% of the sample) on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The first half was treated with 1x SDS loading dye for protein visualisation and incubated at 70°C for 5 minutes and loaded onto a WedgeWell Tris Glycine 4-12% gradient gel (ThermoFisher). Coomassie Brilliant Blue (CBB ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected (5 minutes, 1,000 rpm, 4°C) and RNA was isolated using the PureLink™ RNA Mini Kit (Ambion). RNA concentration was measured using NanoDrop 8000 (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed in serum-free cRPMI medium and treated with 5 nM Fluo-4 calcium indicator dye (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 4–5 × 104 cells were plated per well in 8-well Lab-Tek II Chamber Slides (Nunc, Rochester, NY, USA) one day before experiments ...
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bioRxiv - Immunology 2024Quote: ... heat-denatured at 95°C for 5 minutes and loaded onto a 4-12% gradient premade 1.0 mM Bis-Tris gel (Life Technologies) with molecular weight standard SeeBlue Plus2 (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Sorted iNKT cells were cultured in cRPMI in 96-well plates that were previously coated for 12 h at 4 °C with 5 μg/mL of anti-CD3ε (clone 145-2C11, Invitrogen) in PBS ...
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bioRxiv - Microbiology 2024Quote: ... Two skin swab samples were taken in a 5 mL centrifuge tube and mixed with 1 mL of pre-cooled methanol-water (V:V=4:1, Thermo Fisher), after brief sonication in ice water for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... 5 or 50 ng of each sample was loaded per lane on NuPAGE 4-12% Bis-Tris SDS-PAGE (Invitrogen) in 1X NuPAGE MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 300 µL of dense rosette cultures were first stained for ECM with fluorescein-jacalin (Eurobio Scientific FL-1151-5) and for membranes with FM 4-64 (Invitrogen T13320 ...
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bioRxiv - Neuroscience 2024Quote: ... pH = 7.6) followed by an overnight incubation at 4°C with 1:1500 anti-mouse Tau-5 antibody (Invitrogen #AHB0042) in superblock blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... they were dissociated with 0.5 mM EDTA in DPBS and split every 4-5 days at a ratio 1:8-1:12 using Revitacell Supplement (Gibco). Fibroblasts and early passage iPSCs were cultured at 37°C in 5% CO2 and frozen at passage 4-5.
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bioRxiv - Biochemistry 2024Quote: ... 6 μg of cross-linked sample was loaded per lane and separated for 5 min on a 4-12% Bis-Tris gel (Invitrogen) using MOPS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were boiled at 95°C for 5 min and separated by SDS-PAGE electrophoresis using 4-12% Bis-Tris 80 NuPage gels (Invitrogen) at 180V for 70 min ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were heated for 5 min at 95°C and loaded on a 4-12% Tris-Glycine gel (XP04122BOX, Invitrogen). The transfer onto a PVDF membrane (IB24001 ...
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bioRxiv - Microbiology 2021Quote: ... using 6 mL of cell suspension and 6 mL of Prolong Gold Antifade mountant (ThermoFisher Scientific) that were mixed on an object glass and then covered with a special STED cover glass ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Plasma IL-6 levels were measured by mouse IL-6 ELISA kit (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Developmental Biology 2022Quote: ... bTSCs were passaged every 6 days at a 1:6 split ratio using Accutase (Gibco, A1110501). Each well of bTSCs was dissociated by 1 mL Accutase for 5 mins at 38.5 °C ...
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bioRxiv - Immunology 2022Quote: ... IL-6 measurement was performed using IL-6 Mouse Uncoated ELISA Kit (88-7064-22; Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 105 – 4 x 105 mESCs were cultured in 60mm Petri dishes in DFNB medium (Neurobasal medium/Gibco, DMEM F12 1:1/Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent indicators (fluo-4 AM, fura-2 AM, fura-FF AM) were purchased from Molecular Probes (Thermo Fisher Scientific). NMDA was from Merck Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... were resolved on precast NuPAGE 4-12% Bis-Tris midi 12+2-well protein gels (cat. no. WG1401BOX, Invitrogen) at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Systems Biology 2021Quote: ... Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco, UK, 20h, +4°C) digestion ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... transfected with respective siRNAs (200 pmol/ well) on day 2 and day 4 using oligofectamine (Invitrogen; 10 μl/ well), and processed on day 6 for IFM or biochemical analyses ...
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bioRxiv - Cell Biology 2021Quote: ... the insoluble pellet fractions (2 A260 units) were separated on NuPAGE 4-12% Bis-Tris 15-well gels (Invitrogen), run in NuPAGE 1x MOPS SDS running buffer (Novex) ...
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bioRxiv - Systems Biology 2021Quote: ... Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco, UK, 20h, +4°C) digestion ...
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bioRxiv - Genetics 2021Quote: ... overnight at 4°C and then for 2 hours with Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen 11201D). After 3 800uL washes in LiCl buffer (100mM Tris HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... loaded with 2-4 μM Fluo-5N and incubated with 200 nM MitoTracker® Red CMXRos – M7512 (Invitrogen, USA). To load Fluo-5N ...
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bioRxiv - Cancer Biology 2022Quote: ... Mesenteries were then stored for up to 48hrs at 4°C into PBS with 2% Antibiotic-Antimycotic solution (ThermoFisher).