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Citations for Thermo Fisher :
5251 - 5300 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1 μl of peptide mixture was loaded onto an Acclaim µ-Precolumn enrichment column (0.5 mm x 3 mm, particle size 5 microns, Thermo Scientific, USA) at a flow rate of 10 µl/min for 5 min in isocratic mode with a 2% acetonitrile ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed 3 × 5 min with TBS-T and proteins detected using Chemiluminescent Substrate (SuperSignal™ West Pico PLUS, Thermo Scientific) by acquiring images with Chemidoc (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... the cells were washed 3×5 min with PBS and incubated with Alexa Fluor® -conjugated secondary antibodies (Life Technologies, Eugene, Oregon) at 1:200 ...
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bioRxiv - Genomics 2022Quote: ... 50,000 of mESC+Cas9 cells were transfected with 500ng of each sgRNAs (5 and 3’ sgRNAs) using Lipofectamine 2000 (Thermo Fisher Scientific), and cells were selected with 1μg/ml puromycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified 5’ NotI-APEX2-XhoI 3’ fragments were digested and ligated into the vector pcDNA5/FRT/TO (Invitrogen, Thermo Fisher Scientific). Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified 5’ NotI-APEX2-XhoI 3’ fragments were digested and ligated into the vector pcDNA5/FRT/TO (Invitrogen, Thermo Fisher Scientific). Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and 1492R (5’-TACGGYTACCTTGTTACGACTT-3’) [29–31] with the Invitrogen Platinum II Taq Hot-Start DNA Polymerase kit (Cat. No. 14966001, Invitrogen, Waltham, MA). The 16S rRNA gene amplicons were sequenced by GENEWIZ (Azenta Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... Gateway entry clones of the purified 5’-flank and 3’-flank PCR fragments were generated as described by the manufacturer (Invitrogen, Carlsbad, CA). The hygromycin resistance cassette (hygB ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated product was amplified with a G-quadruplex-specific common primer (NGS-1290, 5’-GGAGGGGAGGGGAGAGGGG-3’) and an adaptor-specific common primer for 12 cycles with Phusion U (Thermo Fisher, #F555L). One-tenth of the amplification product was subjected to another round of amplification using Illumina P5/P7 primers ...
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bioRxiv - Immunology 2024Quote: ... for 13 hours at 37 °C and 5% CO2 in the presence of Brefeldin A (3 µg/mL; ThermoFisher; Waltham, MA, USA) for the last 10 hours of stimulation ...
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bioRxiv - Genetics 2024Quote: ... flanked by 1,400 bp-long sequences homologous to the mouse Dysf locus flanking the murine Dysf exon 44 (5’ and 3’) were purchased as GeneArt synthetic fragments readily inserted into the pMX vector backbone (Thermo Fisher Scientific) (Supplementary Fig ...
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bioRxiv - Cell Biology 2023Quote: ... were seeded on the top of the membrane and cultured for 3 days in DMEM/F12 (1/1) supplemented with 5 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France), 0.2 ng/ml EGF (Gibco) ...
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bioRxiv - Physiology 2024Quote: ... R-5’-3’ UGGUUCAUCAGCCUUUAACTT) and mock siRNA (General Biosystems, China) were transfected into mouse DCT cells by Lipofectamine™ 3000 (Invitrogen, L3000015) (20 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were separated on an Ultimate 3000 HPLC with loading on a 5 cm C18 trap column Acclaim PepMap 100 (3 µm particle size, 75 µm diameter; Thermo Scientific, 164946) followed by a 15 cm PepMap RSLC C18 EASY-Spray column (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of samples were spotted on a coverslip and immobilized by 3 μl of ProLong Diamond (Life Technologies, Mississauga, ON, Canada). Samples were examined at room temperature with a ×63/1.4 oil objective under structured illumination microscopy using an Elyra PS1 microscope (Carl Zeiss ...
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bioRxiv - Immunology 2022Quote: ... the sample was blotted for 2.5 seconds at a ‘blot force’ of −3 and plunge frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific). Electron micrograph movies were collected on a 300 keV Titan Krios (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: A DNA fragment encoding the MAD7 ORF including regulatory sequences (J23119 promoter and T7-terminator) and 5’ and 3’ overlaps with adjacent sequences of the NcoI site in pKD46 was synthetized by GeneArt (Thermo Fisher Scientific). pRED-MAD7 was constructed by integration of this fragment into NcoI-linearized pKD46 (Datsenko and Wanner ...
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bioRxiv - Developmental Biology 2022Quote: ... A cocktail solution containing 50ng/μl gRNA (Integrated DNA Technologies) (5’-ACACTCGTCTCACCAACAGCCGG-3’) and 100ng/μl Cas9 mRNA (A29378, Thermo Fisher Scientific) was injected into one blastomere of two-cell embryos so that resulting embryos would be mosaic for the Fzd2 mutation with at least 50% of the cells being wild-type ...
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bioRxiv - Cancer Biology 2023Quote: ... Both Brca2+/+ and Brca2-/- cell lines were cultured under low oxygen conditions (3% O2, 5% CO2, 37°C) using DMEM (GIBCO; 41966-029) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... The Nitrocellulose paper was washed 3 times for 5 minutes in PBST and chemiluminescent substrate (SuperSignal West Duro Extended Duration Substrate Thermo Scientific #34075) was applied ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for two hours at room temperature with 5% nonfat dry milk (Lab Scientific bioKEMIX Inc., Danvers, MA) and 3% bovine serum albumin (Thermo Fisher Scientific) in 1X Tris-Buffered Saline (Quality Biological ...
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bioRxiv - Immunology 2024Quote: ... Whole- mounts were then washed 3 times for 5 min at room temperature in PBS followed by incubation with Alexa Fluor 568 anti-rat (Invitrogen, 1:500) for 1 hour at room temperature in PBS with 1% BSA and 0.5% Triton-X-100 ...
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bioRxiv - Genetics 2024Quote: ... cDNA was prepared with 400 ng total RNA using specific vector backbone PT2 primer (5’-AAGCTTGCATCGAATCAGTAG –3’) and Superscript III reverse transcriptase (Thermo Fisher, 18080085). PCR amplification of cDNA samples was performed with GoTaq flexi (PROMEGA ...
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bioRxiv - Biochemistry 2024Quote: ... Chemiluminescence was induced by incubating the membranes for 3-5 minutes in the SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Scientific, 34580). Images were captured through a gel imager (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were again washed with PBS (3×5 mins) and then mounted using ProLong™ Diamond Antifade Mountant without DAPI (Thermo Fisher). The images were scanned using VS120 whole slide scanner (Olympus USA ...
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bioRxiv - Plant Biology 2024Quote: ... The SSU-PaCrtI gene was chemically synthesized and incorporated bordering ’5’ (NcoI) and 3’ (AvrII) restriction sites cloned into the pMat intermediate plasmid (Life Technologies, Germany). The FiLUC gene was excised from pT35enh::FiLUC-NosTMod using NcoI/AvrII and replaced with PaCrtI to create the pT35enh::SSU-PaCrtI binary vector.
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bioRxiv - Plant Biology 2024Quote: ... sequence variants downstream of a min35s domain that incorporated 5’ XhoI and 3’ NcoI restriction sites were synthesised and cloned into the pMat intermediate plasmid (Life Technologies, Germany). Fragments were subsequently removed from pMat and cloned into pT35enh::FiLUC binary vector using NcoI/XhoI restriction endonuclease digestion and T4 ligation as previously described (Cazzonelli and Velten ...
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bioRxiv - Immunology 2024Quote: ... 3-5 µg/mL of mDCIR1-ECD or mDCIR1-ΔECD (pre-conjugated with ¼ molar equivalent of streptavidin-HRP; Thermo Fisher Scientific #21124) diluted in TSM was incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 11 μL of nucleic acid was used in a reverse transcription reaction with 100 pmol of primer FR20RV-12N (5’-GCCGGAGCTCTGCAGATATCNNNNNNNNNNNN-3’) using SuperScript IV reverse transcriptase (Thermo Fisher Scientific), followed by second-strand synthesis using the Klenow Fragment of DNA polymerase (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... denatured at 95°C for 5 min and run on 3–8% NuPAGE Tris-acetate gels in NuPAGE Tris-acetate SDS Running Buffer (20x) (Thermo Fisher Scientific) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... After 3× washing of 5 min in TBS-T antibody detection was performed by HRP-ECL chemiluminescence reaction (#32109, Thermo Fisher Scientific) using the Fusion FX 7 (Vilber Lourmat GmbH ...
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bioRxiv - Developmental Biology 2024Quote: ... An RNA/DNA mix was obtained by retrotranscribing 200pmol of RNA + 200pmol of the Reserve primer (5’-TTGTCGCACGTTCGGTGTCG-3’) with Maxima H Minus Reverse Transcriptase (Thermo Scientific, EP0753) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... JEV-5′UTR-cHP-cCS-FLuc or JEV-5′UTR-cHP-cCS-FLuc-3′UTR was respectively transfected into BHK-21 cells using Lipofectamine MessengerMax reagent (Thermo Fisher Scientific). At 12 hpt ...
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bioRxiv - Biochemistry 2024Quote: ... and membranes were washed 3×5 minutes in 1X TBS-T before being developed using Pierce SuperSignal West Pico PLUS (Thermo Scientific, 34580) or Pierce SuperSignal West Femto PLUS (Thermo Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Cells are washed 3 × 5 min in PBS and finally mounted on microscope slides with mounting medium containing DAPI (P36962; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... TMEM67 Δ342 and N-331 constructs were cloned into the HindIII(5’) and XhoI(3’) restriction enzyme sites of the pSecTag2C vector (Invitrogen, V900-20) following overhang PCR amplification of the human Fl-TMEM67-Myc construct using Phusion High-Fidelity Taq DNA Polymerase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... microRNA was modified for 3’ poly(A) tailing and 5’ ligation of an aptamer sequence by TaqMan Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific) to extend mature microRNAs present in the samples on each end prior to reverse transcription ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... TOPRO-3 (Invitrogen) was added to the staining buffer at a dilution of 1:10000 ...
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bioRxiv - Biophysics 2021Quote: ... followed by three washes with phosphate-buffered saline (PBS; Thermo Fisher Scientific, Germany) for 10 min each.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with Dulbecco’s phosphate-buffered saline (PBS, #14190, Invitrogen, USA) and 2% foetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... which was then diluted in phosphate buffered saline (PBS, Thermo Fisher Scientific, UK) with 2% foetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ACE2 variants were serially diluted in Dulbecco’s Phosphate Buffered Saline (DPBS)(Gibco™) using half-log dilutions starting at 31,579 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... washed in phosphate-buffered saline (PBS) and lysed in RIPA lysis buffer (Invitrogen) added with Benzonase and protease inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed with phosphate buffered saline before addition of DAPI (ThermoFisher Scientific) and were examined under a Leica confocal laser scanning microscope ...