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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... in Essential 6 medium (Gibco, A1516401) supplemented with two SMAD pathway inhibitors – dorsomorphin (2.5 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... The QuantStudio 6 system (ThermoFisher Scientific) was employed for running the qRT-PCR experiments ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6-well plates (Nunc, Denmark).
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bioRxiv - Neuroscience 2023Quote: ... Hoescht 33342 (6 µg/mL; Invitrogen) was used to identify nuclei ...
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bioRxiv - Genomics 2023Quote: ... and QuantStudio 6 FLEX machine (Thermofisher). Antibodies used for immunoprecipitation were ...
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bioRxiv - Genomics 2023Quote: ... and QuantStudio 6 FLEX machine (Thermofisher). Antibodies used for immunoprecipitation were ...
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bioRxiv - Immunology 2022Quote: ... IL-6 (100 ng/ml, Invitrogen), TNF-α (10 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 (Thermo Fisher Scientific, #P620), P16 (SCBT ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM magnesium chloride (Invitrogen) was assembled ...
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bioRxiv - Microbiology 2024Quote: ... a 6% DNA retardation gel (Thermofisher) was pre-run at 90 V for 5 minutes with 0.5X TBE buffer (prepared from 5X TBE buffer (Thermofisher)) ...
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bioRxiv - Immunology 2024Quote: ... in 6-well plates (Thermo Scientific) at 1.5x106 cells per well for 1 h at 37°C in warm RPMI-1640 medium (GIBCO) ...
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bioRxiv - Cell Biology 2024Quote: ... 6) glucose-free DMEM (Gibco, 11966025) for 24 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM magnesium chloride (Invitrogen) was added ...
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bioRxiv - Neuroscience 2024Quote: ... and QuantStudio 6 Flex system (ThermoFisher). The cDNA amplification cycle number was determined by ∼25% of the peak fluorescence value ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... with 4% sucrose/4% formaldehyde (ThermoFisher Scientific) in 1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 x g for 1 hour, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was incubated with 2 ml Ni-NTA resin (Thermo SCIENTIFIC #88222 ...
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bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific, Scornsby, VIC, AUS), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 3×105 HEK293T cells were seeded into 6-well plates and transfected with 20 nM siRNA using 5 μL lipofectamineTM RNAiMAX reagent (Thermo Fisher Scientific, cat. no. 13778150) for 48 h according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were acid digested on a hot plate using 6 mL Primar Plus grade HNO3 (68%) and 2 mL H2O2 (Thermo Fisher Scientific, Loughborough, UK). Samples were diluted with Milli-Q water (18.2 MΩ cm ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Immunology 2024Quote: ... 80uL of the solution containing the cells were centrifuged on 12 mm cover glasses using a cytospin centrifuge (6 min at 40 g, Cytospin 2, Thermo Fisher Scientific, Schlieren, Switzerland). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... 4 mM 4-acetamido-4’- maleimidylstilbene-2,2’-disulfonate (AMS) (Invitrogen, catalogue no. A485), and then keep in the dark at room temperature for 1.5 hours ...
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bioRxiv - Neuroscience 2022Quote: ... We incubated overnight with the anti-GFP primary antibody at 4 degrees (1:1000, A-6455 Invitrogen, 0.1M PBS 0.3% tx100, 3% NGS). After abundant PBS washes ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted total RNA from 3-4 biological replicates per sex and line with a PureLink RNA purification kit (Thermo Fisher Scientific, USA) and validated the samples with the Bioanalyzer RNA 6000 Nano kit (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and two solid-phase extraction columns (150 µm × 3 cm) packed with MAbPac particles (C8 resin, 4 µm diameter, 300-Å pore size; Thermo Fisher Scientific) were used ...
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bioRxiv - Cell Biology 2023Quote: ... and lentiviral vector expressing GFP-UMOD-WT or GFP-UMOD-H177- R185 del at a ratio of 3:1:4 using Lipofectamine 3000 Reagent (ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the packaging plasmids psPAX2 and pMD2.G at a ratio 4:3:1 in the presence of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The supernatants containing lentiviral particles were collected 48 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated at room-temperature for 3-4 hours with secondary antibody mixture containing Alexa Fluor 568 anti-mouse (1:500; Thermo Fisher Scientific, A11031) and Alexa Fluor 647 anti-chicken (1:500 ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with IgG for 3-4h at 4 degrees before the overnight incubation with human TROP2 antibody (Invitrogen, 14-6024-82) or mouse TROP2 (R&D ...
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bioRxiv - Cancer Biology 2024Quote: ... indicated with “dry” were stored on activated 3 Å (EtOH, MeCN) or 4 Å (other solvents) molecular sieves (8 to 12 mesh, Acros Organics).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were denatured at 95°C for 3 min and 8 µL of sample was loaded into 4-20 % Tris-Glycine 15-well pre-cast gels (Thermo Fisher Scientific, XP04205BOX). In-gel fluorescent images were acquired using an Odyssey LI-COR.
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bioRxiv - Cancer Biology 2021Quote: ... 5’ CAGACAGUGCAGUAUUCACTT 3’) and control siRNA (#4427037) as in (56) and OSR1 siRNA (s19303 Silencer® Select) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and fed with the complete Lifeline medium plus antibiotics and transfected with 50 nM custom mirVana miRNA inhibitor (inhibitor sequence: 5′-GAGCCCGACGAGCUACCAGACUGCUCCA-3′, #4464086, Thermo Fisher Scientific) or 50 nM mirVanna inhibitor negative control#1 (#4464077 ...
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bioRxiv - Genetics 2021Quote: ... and subsequently counterstained with DAPI (1:30,000 of 5 mg/ml) for 3 minutes and mounted using Antifade reagent (ProLong Gold Antifade Reagent, Invitrogen, cat. number P36934). Images were taken and processed using an Airyscan980 SR confocal microscope (Zeiss).
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bioRxiv - Genetics 2021Quote: ... the extracted DNA was directly Sanger sequenced from in 5’ and 3’ directions for each sample with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol with the same primers as for the amplification (Prdm9ZnFA_Bal_R/Prdm9ZnFA_Bal_F) ...