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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: Dried peptide samples were dissolved in 4.8 μL of 0.25% formic acid with 3% (vol/vol) acetonitrile and 4 μL of each sample was injected into an EasynLC 1000 (Thermo Fisher Scientific). Peptides were separated on a 45 cm in-house packed column (360 μm OD×75 μm ID ...
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bioRxiv - Molecular Biology 2023Quote: ... Uterine tissues were cut into 3- to 4-mm lengths with sterile scissors and incubated in phenol red-free HBSS(−) containing dispase (#17105-041, Thermo Fisher Scientific) and pancreatin (#P3292 ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed in Ca2+-free PBS and incubated for 20 min at room temperature with 3 μM Fluo-4-AM (F14201, Thermo Fisher Scientific) in PBS while being protected from ambient light ...
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... for a 35 mm dish 4 μl of Lipofectamine 2000 and selected plasmids (3 μg) were diluted in Opti-MEM medium (Thermo Fisher Scientific) to a final volume of 250 μl and incubated for 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies were incubated on slides in a the dark for 3 hours at room temperature or overnight at 4°C: GαM 488 (Invitrogen A21121, 1:1000), GαRb 546 (Invitrogen A11010 ...
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bioRxiv - Cell Biology 2023Quote: ... were plated into 12-well plate at a density of 3-4 × 105 cells in 1 mL per well in 1x DMEM (Gibco Cat# 11995065) plus 10% FBS (Gibco Cat# 16000044 ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were incubated for 3 h at 4°C under gentle rotation with Pierce Protein G Plus Agarose (Thermo Fisher Scientific) conjugated to antibodies specific for FUS (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... 17,22,43,44 Early passages (P2-4) of cells from 3 rats were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% non-fat milk for 3 hours and incubated overnight at 4°C with primary antibodies against COL1 (1:1000, Invitrogen, PA5-95137), COL3 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Grids were either blotted for 3 or 4 s before being plunged into liquid nitrogen-cooled liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... 1 x 109 SupT1 CD4+ T cells were infected with 4 055 ng of VSV-G-HIVNL4-3 XCS HIV-Gagp24 for 3h in IMDM plus 10mM HEPES (Gibco/ThermoFisher Scientific) supplemented with 2 µg/ml of DEAE-dextran (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples were resolved on NuPAGE 4–12% Bis-Tris gels with MOPS buffer or 3–8% Tris-acetate gels with Tris-acetate buffer (Life Technologies, #LA0041) and then transferred to 0.45 μm nitrocellulose membrane (Amersham) ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was then probed with specific primary antibodies diluted 1:1000 in antibody dilution buffer (3% BSA in TBS-T) and incubated overnight at 4°C - either anti-TNFR1 rabbit monoclonal antibody (Invitrogen, #MA5-14899) or anti-β-Actin mouse monoclonal antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at room temperature (RT) prior to overnight incubation at 4 °C with one of the following primary antibodies: Caspase 3 (Invitrogen, Cat# 700182), Insulin (SCBT ...
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bioRxiv - Cell Biology 2024Quote: ... proteolytic peptides (500 ng for Experiment 3 and 400 ng for Experiment 4) were loaded on Acclaim PepMap 100 C18 trap column (75 µm x 20 mm, 3 µm particle size; Thermo Fisher Scientific) for 10 min at 2 µL/min with 100% solvent A ...
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bioRxiv - Neuroscience 2022Quote: ... A Neuropixels probe (option 3 phase A) with 374 recording sites was briefly (∼2 minutes) dipped into the diI cell-labeling solution (ThermoFisher) to visualize probe tracks ...
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bioRxiv - Molecular Biology 2020Quote: ... medium from the basolateral compartment was renewed every 2–3 days and the apical surface was washed once a week with PBS (Invitrogen).
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Genetics 2021Quote: 2-3 × 106 mESCs were transfected with 14.5 μg of TALE plasmid and 26 μL Lipofectamine 3000 Reagent (Invitrogen L3000015) and seeded onto 0.1% gelatin-coated 10 cm dish containing an autoclaved SuperFrost Plus Adhesion glass slide ...
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bioRxiv - Bioengineering 2020Quote: ... proteins and the Canadian clinical isolate of SARS-CoV-2 (V-2/Canada/VIDO-01/2027 Feb 2020) were mixed in fresh media supplemented with 3% fetal bovine serum (Gibco) and preincubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS prior to loading with 3 μM of the Ca2+ indicator Fura-2 AM (Invitrogen) diluted in a modified Krebs-Ringer buffer [KRBH ...
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bioRxiv - Pathology 2021Quote: ... The tissue was homogenized with a Pasteur pipette in Growth medium [3 ml DMEM-F12 with 2% of B27 without vitamin A (Gibco), 20 ng.mL-1 Epidermal Growth Factor (EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018) using a dT primer and Superscript IV (Invitrogen) reverse transcriptase ...
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bioRxiv - Cell Biology 2021Quote: ... cells were loaded with 3 μM Fura-2-AM and 0.01% (v/v) Pluronic F-129 (both from Molecular Probes) in DMEM medium at 37 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL of total RNA was subjected to One-Step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Bioengineering 2022Quote: ... The medium was replaced every 2-3 days and cells were split once they reached 75-80% confluence using TrypLE Express (Gibco). LNCaP and U87 cells were gifted by Professor Lisa Butler and Professor Stuart Pitson ...
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bioRxiv - Molecular Biology 2020Quote: ... Per2Luc mice and littermate wild-type control mice were euthanized by isoflurane 2-3 hours before lights off (ZT 9–ZT10) and tissues were immediately removed into ice-cold HBSS (Gibco). Coronal brain slices containing SCN (400-µm thickness ...
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bioRxiv - Bioengineering 2021Quote: ... scaffold sequences and 3’ extension sequences were amplified with indicated primers (Supplementary table 2) using Phusion master mix (ThermoFisher Scientifc) or Q5 High-Fidelity enzyme (NewEnglandBioLabs) ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... the media was replaced entirely with fresh retinal differentiation media (RDM) (DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX)) ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2020Quote: ... the media was changed to retinal differentiation medium (RDM;DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ZO-2 pAb (38-9100) and rabbit anti-ZO-3 pAb (36-4100) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were regularly monitored for bacterial contamination by checking for bacterial growth in Zobell marine broth 2216 (HiMedia) (71) in addition to filtering 2-3 mL of exponential-phase growing culture and using Sybr Green I (Invitrogen) staining and epifluorescence microscopy (Nikon Eclipse 80i ...
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bioRxiv - Molecular Biology 2021Quote: ... For cell collection and/amplification ESCs were trypsinized for 2 to 3 minutes with trypsin-EDTA (Invitrogen, Cat#25200-072) and the digestion was stopped by the addition of pre-warmed 15% FCS ...
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bioRxiv - Immunology 2020Quote: ... 2 and 3 cDNA were amplified by real-time PCR using the SYBR Green PCR kit (Thermo Scientific, Braunschweig, Germany). The primer sequences used for the real-time PCR were listed in Table 1.
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bioRxiv - Molecular Biology 2022Quote: 4 L exponentially growing Sf9 cells at a density of 2-3 × 106 cells/mL in Sf900-III SFM media (Invitrogen) was infected with the high-titer baculovirus stock ...
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bioRxiv - Microbiology 2023Quote: ... Five microliters were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nL/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Systems Biology 2023Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Molecular Biology 2023Quote: ... hESCs were differentiated into cardiac mesoderm by culturing for 3 days in RPMI B27-medium (RPMI 1640 GlutaMAX + 2% B27 supplement minus insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma) ...