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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of the extracted RNA samples were reverse-transcribed using a Uni-12 primer (5′-AGCRAAAGCAGG-3′) and Superscript III reverse transcriptase (Thermo Fisher Scientific). Ten-fold diluted cDNAs were PCR amplified using KOD FX (Toyobo ...
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bioRxiv - Cell Biology 2021Quote: ... or siGENOME SMARTpool siRNA D-019800-17 DSP (Dharmacon, Lafayette, CO) (siDP1) or stealth siRNA oligonucleotides 5′-CAGGGCUCUGUCUUCUGCCUCUGAA-3′ from Life Technologies (siDP2) using the Amaxa Nucleofector System (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: ... were co-electroporated with the gRNA-5’/3’-expressing construct and a circular donor plasmid carrying the synthesized knock-in template (GeneArt®Invitrogen™).
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
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bioRxiv - Molecular Biology 2020Quote: ... and then resuspended in aliquots of 3-5×106 CD34+ cells in a total volume of 750ul of Opti-MEM (Thermo Fisher Scientific). An aliquot of RNPs were added to the cell suspension and electroporated using the BTX ECM 830 Square Wave Electroporation System (Harvard Apparatus ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Flow settings were adjusted to allow individual cells to become encapsulated with single hydrogel beads presenting cell-specific barcoding oligonucleotide primers inside 3-5 nL encapsulation mixture droplets that contain 20 U μL−1 reverse transcriptase (SuperScript III, Thermo Fisher Scientific), First-Strand Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: A pair of oligomers containing a 20-bp sgRNA (5’-AAAGACGAGTGATGCCATTT-3’) sequence targeting the surrounding genomic segment of HOXA9 stop codon was synthesized (Thermo Fisher Scientific) and cloned into the all-in-one vector ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL per mL of Expi293F culture) were added to separate 5 mL aliquots of 37°C Opti-MEM (Thermo Fisher Scientific), mixed after 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... Sequencing primer 5’-AAGCTGGAGCTCCACCGCGG-3’ was annealed and sequencing was performed with the USB Sequenase Version 2.0 DNA sequencing kit (Thermo Fisher Scientific - 70770).
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bioRxiv - Cancer Biology 2021Quote: ... The amplified linear products were then annealed to a 5’ adaptor with 7bp 3’ random nucleotide overhang and PCR-amplified using adaptor oligos (Sangon, Shanghai, China) and Phusion (Thermo Fisher Scientific), resulting in a library with proper Illumina sequencing adaptor ends ready for NGS ...
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bioRxiv - Cancer Biology 2021Quote: ... 4μl of the purified PCR products were added to 10μM primers and 2μM probe (forward: TACCGGTTAGTAATGAC; reverse: TAAAGCGCATGCTCCAG; probe: 5’-FAM-AAAAGCGCCTCCCCTACCCGGTAGGTA-3’-Eclipse) and 5μl TaqMan™ Fast Advanced Master Mix (Applied Biosystems™). The following thermocycling program was used ...
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bioRxiv - Cancer Biology 2020Quote: ... R: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGCGCCTCGTGCTGGAGTAG-3’) and reacted with the gateway donor vector pDONR223 by using BP clonase II (11789020, ThermoFisher Scientific, Madrid, Spain). The resulting entry clones containing SRRM4 WT and DM were reacted with the gateway destination vector pCW57.1 (a gift from David Root ...
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bioRxiv - Biochemistry 2021Quote: 10 uL of either Caspase-3 or −7 library glycerol stocks was used to inoculate 5 mL of auto-induction media (Invitrogen Magic Media) and allowed to incubate and express for 18 hours at 30 °C ...
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bioRxiv - Systems Biology 2021Quote: ... Tissue was then washed in 1X PBS 3 × 5 min followed by incubation with Alexa Fluor 488 secondary antibody (Invitrogen, Carlsbad, CA) diluted to 1:500 in Antibody Dilution Buffer (DAKO ...
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bioRxiv - Neuroscience 2021Quote: Viral titer was estimated by qPCR with an MGB-NFQ FAM-labeled probe that recognized GFP (Life Technologies, sequence: 5’-CTTCAAGGAGGACGGCAA-3’) and 20 µg of Hsp68-EGFP vector plasmid as a standard ...
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bioRxiv - Plant Biology 2020Quote: ... qPCRs were carried out on an Applied Biosystems QuantStudio 3 and 5 Real-Time PCR Systems (Applied Biosystems, Foster City, California, USA) using 80 - 150 ng of genomic DNA as template ...
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bioRxiv - Bioengineering 2022Quote: ... PACE60 nanoparticles were formulated as described previously by double emulsion solvent evaporation using miR200b mimic (Invitrogen mirVana miRNA Mimic, hsa-miR-200b-3p, 5’-UAAUACUGCCUGGUAAUGAUGAC −3’, Cat# 4464066, Ambion, Austin, TX) or a negative control miR (Invitrogen mirVana miRNA Mimic ...
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bioRxiv - Cell Biology 2022Quote: ... YFP+-AT2 or YFP+-βcat ΔExon 3 and lung fibroblasts (5 x 104) in growth media containing the following: αMEM media (Thermo Fisher #41061029) supplemented with 4.5 g/l D-glucose ...
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bioRxiv - Cell Biology 2022Quote: ... synthesized to knockdown human GJA1 mRNA (sequence 5’ to 3’: GGGAGAUGAGCAGUCUGCCUUUCGU; cat. # HSS178257) and Stealth™ RNAi (Thermo Fisher scientific, cat. # 12935112) was used as a negative control ...
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bioRxiv - Cancer Biology 2022Quote: ... The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3, eBioscience) for 3◻days in RPMI-1640 (Gibco) supplemented with 10% FBS (Sigma ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... 5 brains or 10 guts of 3-5 days old males were dissected and immediately transferred into 100 μl of ice-cold TRIzol (Thermo fisher #15596026) one by one ...
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bioRxiv - Cancer Biology 2020Quote: ... for target genes was performed using primaQUANT real-time PCR Master Mix (Steinbrenner Laborsysteme, Wiesenbach, Germany) using QuantStudio™ 3 or 5 Real-Time PCR Systems (Applied Biosystems) and Universal Probe Library probes ...
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bioRxiv - Microbiology 2020Quote: ... and a newly designed 5’ FAM-labelled and 3’-minor groove binder (MGB)-modified probe SVIP-MP_P2-MGB ((Table 1; ThermoFisher Scientific, Eurofins Genomics). The primers encompass a 182 nucleotides-long amplicon ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... A second siRNA oligo (McIdas siRNA_2) was also used for McIdas depletion: 5’-CAACTGCACGTGACATTGATT-3’ (s51151, Silencer® Select siRNA, Thermo Fisher Scientific). The Silencer® Select No ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed with Bond Wash solution 3 × 5 minutes and then wet mounted with ProLong Gold Antifade reagent with DAPI (P36931, Invitrogen, Carlsbad, CA) and allowed to dry overnight in the dark ...
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bioRxiv - Bioengineering 2020Quote: The IGFBP-derived NLS peptides (NLS-3 and NLS-5) were extracted from the bacteria using the B-Per 6xHis Fusion Protein Purification Kit (Thermo Scientific, USA), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen, Thermo Fisher Scientific, Waltham, MA) and magnetically stirring the solution in a glass vial at 45 °C ...
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen, Thermo Fisher Scientific, Waltham, MA) and magnetically stirring the solution in a glass vial at 45 °C ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using OC43-nucleocapsid specific TaqMan primers and a probe 18 fluorescent- labelled with a 5’-FAM reporter dye and 3’-BHQ quencher (IDT) and AgPath-ID™ One-Step RT-PCR kit (AgPath AM1005, Applied Biosystems) on an ABI QuantStudio 3 platform (Thermo Fisher) ...
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bioRxiv - Physiology 2020Quote: ... as well as non-specific negative control oligonucleotides (5′-AAATGTACTGCGCGTGGAGAC-3′) were cloned into pcDNA6.2-GW/EmGFP-miR [“BLOCK-iT™ PolII miR RNAi Expression Vector Kit” (Invitrogen, Darmstadt, DEU). Human Flag- and myc-tagged GPT and GPT2 cDNA was obtained from Origene (RC203756 and RC209119) ...
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bioRxiv - Cancer Biology 2020Quote: ... KTB21 cells were cultured in epithelial cell growth medium (DMEM (low glucose):Ham’s F12 (1:3) medium supplemented with 5% FBS (Thermo Fisher Scientific, USA), 0.4μL/mL hydrocortisone (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 0.2 units Klenow 3′→5′exo DNA polymerase (Enzynomics, Daejeon, ROK) and 1 μL RNaseH (Invitrogen, San Diego, CA). The Klenow reaction mixture was incubated at 37°C for 1 hr and 75°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Both halves of MF filters and entire SF filters were transferred independently to 5 mL Eppendorf tubes and 3 mL of autoclaved PBS pH 7.4 (1X) (Gibco™, Thermo Fisher) was added ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed 3 x 5 minutes in PBS and stained with secondary antibodies donkey anti-rabbit Alexa Fluor 555 (1/1000, Invitrogen, A-31572) and donkey anti-goat Alexa Fluor 488 (1/1000 ...
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bioRxiv - Immunology 2020Quote: ... slides were washed 3 times for 5 minutes with PBS and mounted with Prolong Gold antifade reagent with DAPI from Invitrogen (Catalog #36935), covered with a coverslip and allowed to cure ...
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bioRxiv - Genomics 2021Quote: ... was used to generate entry clones of the purified 5’-flank and 3’-flank PCR fragments as described by the manufacturer (Invitrogen, Carlsbad, CA). The hygromycin resistance cassette (hygB ...
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bioRxiv - Genomics 2021Quote: ... Each tissue was represented by 2-3 individual replicates that were each derived by pooling tissues from 3-5 mice and FACS sorting cells directly into TRIZOL (Invitrogen, cat. 15596026). In some cases ...
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bioRxiv - Microbiology 2020Quote: ... supernatant discarded and cells washed with 1 ml of Phosphate saline buffer (PBS) and collected again by 3 min centrifugation at 5 000 rpm in a microcentrifuge Heraeus Fresco 21™ (Thermo Scientific). Cells were then counted on a Neubauer chamber to inoculate 106 cells in 1 L of the different media used to obtain the specific morphology.
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bioRxiv - Microbiology 2022Quote: ... 5 μl of samples were spotted on a coverslip and immobilized by 3 μl of ProLong Diamond (Life Technologies, Mississauga, ON, Canada). Samples were examined at room temperature with a ×63/1.4 oil objective under structured illumination microscopy using an Elyra PS1 microscope (Carl Zeiss ...
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bioRxiv - Immunology 2022Quote: ... the sample was blotted for 2.5 seconds at a ‘blot force’ of −3 and plunge frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific). Electron micrograph movies were collected on a 300 keV Titan Krios (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: A DNA fragment encoding the MAD7 ORF including regulatory sequences (J23119 promoter and T7-terminator) and 5’ and 3’ overlaps with adjacent sequences of the NcoI site in pKD46 was synthetized by GeneArt (Thermo Fisher Scientific). pRED-MAD7 was constructed by integration of this fragment into NcoI-linearized pKD46 (Datsenko and Wanner ...
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bioRxiv - Developmental Biology 2022Quote: ... A cocktail solution containing 50ng/μl gRNA (Integrated DNA Technologies) (5’-ACACTCGTCTCACCAACAGCCGG-3’) and 100ng/μl Cas9 mRNA (A29378, Thermo Fisher Scientific) was injected into one blastomere of two-cell embryos so that resulting embryos would be mosaic for the Fzd2 mutation with at least 50% of the cells being wild-type ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed 3 × 5 min with TBS-T and proteins detected using Chemiluminescent Substrate (SuperSignal™ West Pico PLUS, Thermo Scientific) by acquiring images with Chemidoc (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... the cells were washed 3×5 min with PBS and incubated with Alexa Fluor® -conjugated secondary antibodies (Life Technologies, Eugene, Oregon) at 1:200 ...
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bioRxiv - Genomics 2022Quote: ... 50,000 of mESC+Cas9 cells were transfected with 500ng of each sgRNAs (5 and 3’ sgRNAs) using Lipofectamine 2000 (Thermo Fisher Scientific), and cells were selected with 1μg/ml puromycin (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... R-5’-3’ UGGUUCAUCAGCCUUUAACTT) and mock siRNA (General Biosystems, China) were transfected into mouse DCT cells by Lipofectamine™ 3000 (Invitrogen, L3000015) (20 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated product was amplified with a G-quadruplex-specific common primer (NGS-1290, 5’-GGAGGGGAGGGGAGAGGGG-3’) and an adaptor-specific common primer for 12 cycles with Phusion U (Thermo Fisher, #F555L). One-tenth of the amplification product was subjected to another round of amplification using Illumina P5/P7 primers ...
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bioRxiv - Microbiology 2024Quote: ... and 1492R (5’-TACGGYTACCTTGTTACGACTT-3’) [29–31] with the Invitrogen Platinum II Taq Hot-Start DNA Polymerase kit (Cat. No. 14966001, Invitrogen, Waltham, MA). The 16S rRNA gene amplicons were sequenced by GENEWIZ (Azenta Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... Gateway entry clones of the purified 5’-flank and 3’-flank PCR fragments were generated as described by the manufacturer (Invitrogen, Carlsbad, CA). The hygromycin resistance cassette (hygB ...