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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Embryos were incubated for 1hr at 28ºC with 400 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen Cat# A5040) and 5% DMSO in E3/PTU ...
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bioRxiv - Immunology 2024Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.5-0.75 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen). Lipofection was done using small volumes of Opti-MEM (200 μL ...
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bioRxiv - Systems Biology 2024Quote: ... followed by reduction and alkylation in 5 mM tris(2 - carboxyethyl)phosphine (TCEP) (Thermo Fisher, Cat No. 77720) and 50 mM 2-Chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2024Quote: ... The same test was done in parallel to assess the cell viability using MTT at 5 ug/mL (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Invitrogen). Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517 ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2-5 mg of minced tissue was loaded into a cuvette containing Amplex Red (Invitrogen, Waltham, MA, USA), horseradish peroxidase (5U/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... and then nutated in Grace’s Insect Medium containing 0.25 mg/ml 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) for 30 min at room temperature to label S-phase cells ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... One liter of Sf9 cells (2 × 106/mL confluence) was infected with 5 mL P3 baculoviral stock obtained following the manufacturer’s instructions (Invitrogen). The medium containing secreted protein was collected on the 4th day (about 72-hour post-infection ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... #2) Sterilize the oxygen sensor by submerging the sensor tip in 70% ethanol (Thermo Fisher Scientific, 64-17-5) in an eppendorf tube for 10 sec ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Bioengineering 2020Quote: ... The marrow was flushed out of the bones via 2 - 5 mL 4 °C DPBS (Dulbecco’s Phosphate-Buffered Saline; Cat. No. 21-031-CV; Gibco) injection ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Microbiology 2021Quote: ... A trap column (5 μm, 100 Å, 100 μm × 2 cm, Acclaim PepMap 100 nanoViper C18; Thermo Fisher Scientific) and an analytical column (2 μm ...
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bioRxiv - Neuroscience 2020Quote: Time plug-mated Pbx3−/- mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU; 0,1mg/g animal; Invitrogen) at E15.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical astrocytes were plated at a density of 2.5 × 104 to 5 × 104 cells.cm−2 in Minimum Essential Media (Gibco, MEM) supplemented with 10% Horse Serum (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 µL of 20 µM siRNA and 5 µL RNAiMAX were diluted in 400 µL OptiMEM (Thermo Fisher Scientific), mixed gently and incubated for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Cells were then counterstained with DAPI (4,6-diamidino-2-2phenylindole) in 5% BSA with 0.1% Triton X-100 and mounted with Prolong Gold Antifade mounting medium (Invitrogen). Images were then acquired with Olympus Fluoview FV1000 confocal microscope system with PLAPON 60X O SC NA 1.40 ...
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bioRxiv - Immunology 2022Quote: ... IgG was eluted from the resin in 5 × 2 mL fractions using IgG elution buffer (Pierce ThermoFisher, catalog #21004). Fractions were buffer exchanged into PBS ...
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bioRxiv - Cell Biology 2022Quote: Isolated colonic crypts or organoids were loaded at RT with Fura-2/AM (5 μm, 2h; Thermo Fisher Scientific) in HBS ...
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bioRxiv - Cell Biology 2022Quote: ... non-IR and IR animals were systemically injected with 0.9% saline containing 0.25 mg per 25 g of body weight of 5-ethynyl-2’-deoxyuridine (EdU, Thermofisher Scientific) followed by a 2 hour or 7 days chase before sacrifice ...
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bioRxiv - Microbiology 2022Quote: ... A total of 5×105 naïve HEp-2 cells were stained with Vybran DIO Cell-Labeling Solution (0.005 μg/ml, Thermo fisher) for 20 min at 37 °C and washed with PBS ...
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bioRxiv - Biophysics 2022Quote: ... Blocking was performed for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Bovine Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was rinsed in PBS (2 × 5 min) and placed in a 9 mm wide × 0.5 mm deep gasket (Invitrogen) on a glass slide that was previously coated with Poly-L-Lysine (1 µl ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% ACN / 0.1% formic acid at a flow of 250 nl/min for 120 min and analyzed with a QExactive Plus mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Biochemistry 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile / 0.1% formic acid at a flow of 250 nL/min for 120 minutes and analyzed with a QExactive Plus mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Samples of 5 μL were loaded onto a trapping column (Acclaim PepMap100 C18, 75 μm × 2 cm, Thermo Scientific) using 96% eluent A (0.1% formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μg of each vector were used to transfect 5×105 MC38 cell line with Lipofectamine 2000 (ThermoFisher Scientific) in Opti-MEM medium (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and 2 μL of each culture were spotted onto a TSA plate containing 5% sheep’s blood (Thermo Fisher, R060312). The plates were incubated at 30°C for 72 h and imaged above a white light ...
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bioRxiv - Immunology 2020Quote: ... The SYBR green qPCR reactions contained 5 µl of 2× Maxima SYBR green/Rox qPCR Master Mix (K0221; ThermoFisher), 5 µl of diluted cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... incubated 5 minutes with at room temperature with 500 ng/ml 4’,6-Diamidino-2-Phenylindole Dilactate (DAPI; ThermoFisher) in DPBS and mounted in ProLong®Gold (ThermoScientific).
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bioRxiv - Microbiology 2021Quote: ... Samples for DNA and RNA analyses were filtered (∼2 to 5 L) through triplicate 0.2 and 0.1 μm filter towers (Thermo Scientific™ Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with CN Membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Genomics 2020Quote: The eluted cDNA:mRNA hybrids (15 μl) were combined with second strand mix [2 μl 5× First Strand Buffer (Invitrogen), 2.31 μl Second Strand Buffer (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: mRNA from 2-5 x 104 sorted cells was captured with 12 μl of Dynabeads oligo(dT) (Life Technologies), washed ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspendend in 1 mL M9 before 5 µL of DiI stock solution (2 mg/mL DiI (Molecular Probes, #D282) in dimethyl formamide ...
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bioRxiv - Cancer Biology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% ACN/0.1% formic acid at a flow of 250 nL/min for 90 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 5-ethynyl-2’-deoxyuridine in sterile saline (EdU, Thermo Fisher Scientific, USA, catalog #A10044), at a dose of 50mg/kg mouse on post-MCAO day 3-7 in single daily injections ...
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bioRxiv - Neuroscience 2023Quote: EdU (5-ethylnyl-2’-deoxyuridine) was prepared according to the manufacturer’s recommendations (10mM stock solution in DMSO, ThermoFisher #C10337). This solution was then diluted in sterile PBS to achieve a final concentration of 5mg/kg body weight of the pup when injected ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to ultraviolet light (UV) for 5 minutes with a 2% of sulfa SANPAH solution (Thermo scientific, 22589). The hydrogels were washed several times under sterile conditions and incubated with 3.3% of Rat Tail Collagen 1 (Gibco A10483 ...
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bioRxiv - Physiology 2022Quote: Adipocyte differentiation was induced by treating confluent cells for 2 days with 5 mg/ml insulin (Thermo Fisher Scientific) and 10μM ETYA (Santa Cruz Biotech ...
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bioRxiv - Bioengineering 2023Quote: ... 5 × 105 HSkMC cells were incubated with 2 μg of eGFP-mRNA packaged in LPP or lipofectamine (Thermo Scientific) for 24 h in 6-well plate ...