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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Cell Biology 2021Quote: ... and the suspension diluted 1/5 by adding FACS buffer (FB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were maintained in culture (37°C, 5% CO2) fed by Neurobasal-A media supplemented with 2 mM L-glutamate (GlutaMAX, Gibco) 2 % B27 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were kept in retrieval solution until reaching room temperature and washed 2 × 5 min with Tris-buffered saline (TBS; ThermoFisher) plus 0.3% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated in DAPI (used at 2 μg/ml in PBS) for 5 min to counterstain nuclei and mounted in Prolong Gold antifade reagent (Invitrogen). Proprietary (Advanced Cell Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm, 5 µM particles, Thermo Scientific) at a flow rate of 5 µl/min using mobile phase A (0.1% formic acid and 1% acetonitrile in water) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then diluted to 0.5-2 mg/ml in the hybridization solution with 5% dextran sulfate (Cat. No. S4030; Thermo Fisher). Fluorescence RNA in situ hybridization procedure was performed following Thisse and Thisse (2008 ...
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β-importins Tnpo-SR and Cadmus and the small GTPase Ran promote ovarian cyst formation in DrosophilabioRxiv - Developmental Biology 2021Quote: ... dissected ovaries were incubated for one hour at room temperature in Grace's media containing 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Life Technologies). The following primary antibodies were diluted in blocking solution and used overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Roughly 2–5 μL of DNA (~80 ng/mL concentrations) were slowly pipetted into ~50 μL of competent cells (Invitrogen). The cells were left on ice for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... then resuspended at 2 × 107 cells/mL in growth medium supplemented with 5 mM biotin-phenol (biotin tyramide, Acros Organics). After a one-hour incubation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
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bioRxiv - Immunology 2023Quote: ... for 2 h at 37 °C and 5% CO2 in the presence of 0.5 mg/ml dextran-rhodamine (Molecular Probes). Untreated Pf sporozoites were used to measure maximum traversal capacity ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: hiPSC-ECTs were then introduced to a Fura-2 loading solution consisting of Krebs–Henseleit buffer supplemented with 5□μM Fura2-AM (Invitrogen) and 1% (vol/vol ...
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bioRxiv - Cancer Biology 2022Quote: The 5-ethynyl-2’-deoxyuridine (EdU) assay was performed following the manufacturer’s instructions (Thermo Fisher Scientific, C10633 Alexa Fluor 488). We used 10μM of Edu for a 2-hour incubation ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was assessed using the 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay (Click-iT EdU Alexa Fluor 488 Imaging Kit, Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were injected on a PepMap 100 C18 trap column (100 µm × 2 cm, 5 µM particles, Thermo Fisher Scientific) and separated with an EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Biochemistry 2023Quote: ... dUTP was added to DNA ends by pelleting stored cells and resuspending in 100 μL of elongation buffer (1X terminal deoxytransferase [TdT] buffer, 2.5 mM CoCl2, 0.3 mM 5-bromo-2’-deoxyuridine [BrdU] [Invitrogen, Waltham, MA]), then adding 5 U of TdT (Thermo-Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... FACS buffer (5% bovine serum albumin [BSA], 2 mM EDTA, and FluoroBrite Dulbecco’s modified Eagle’s medium [Thermo Fisher Scientific, A1896701]) was used for diluting the antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... and human phosphatidylinositol 5-phosphate 4-kinase type-2 beta (hPIP4KB; Uniprot #P78356) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). Gene sequences were subcloned into either bacterial ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 µm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Developmental Biology 2023Quote: Aiptasia or Nematostella polyps were relaxed for 15 min in 0.1M MgCl2 in sea water or Nematostella medium and transferred to sea water or Nematostella medium containing 300 µM EdU (5-ethynyl-2′-deoxyuridine, Invitrogen) 2%DMSO 0.1M MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific] ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated using 50 cm Acclaim PepMap 100 analytical column (75 μm ID, 3 μm C18) in conjunction with a Pepmap trapping column (100μm × 2 cm, 5 μm C18) (Thermo Scientific) analysed with Orbitrap Fusion Tribrid mass spectrometer (Thermo-Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions (5 × 105 - 2 × 106 total cells) were washed with PBS and resuspended in 1X Annexin binding buffer (Thermofisher) and washed once ...
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the full volume of media in each well was pipetted gently 4-5 times and added to 1 mL of FACS buffer containing 3 uM DAPI (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA, 3 uM DAPI (Thermo Scientific #62247)) ...
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bioRxiv - Immunology 2024Quote: ... were grown as a monolayer at 37°C with 5% CO2 in RPMI 1640 supplemented with 2 mM L-glutamine (31870-025, Thermofisher), 1 mM sodium pyruvate (11360-039 ...
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bioRxiv - Microbiology 2023Quote: ... Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Fisher) solution ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, 164199, ThermoFisher) and separated on an in-house packed analytical column i.d ...
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bioRxiv - Genetics 2023Quote: ... 2–5 μg of total RNA was reverse transcribed using a Superscript First-Strand Synthesis system (Invitrogen, Carlsbad, CA, USA). The quantitative RT-PCR was performed using the suh1-4 RT primers (Supplementary Table S2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated in a 5% CO2 environment at 38.5°C in 2 mL RPMI 1640 media (Gibco, UK) with 10% FBS (Gibco One-Shot FBS ...
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bioRxiv - Physiology 2023Quote: ... was washed with wash buffer II (150mM NaCl, 50mM Tris pH 7.5, 5% glycerol) on the DynaMag-2 magnet (ThermoFisher Scientific) and added to each lysate and rotated for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... TEV (2): 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of AcTEVTM protease (10 U/µL) (Invitrogen) in SEC buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...