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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) for 45 min in 1X blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 × 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Linear Trap Quadrupole Orbitrap Velos (with CID ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with PBS 0.1% Triton X-100 for 5 minutes and nuclei were counterstained with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS 0.1% Triton X-100 for 5 minutes at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... freshly cultured hESC colonies were dissociated into clumps (2-5 cells) with Versene buffer (with EDTA, Gibco, Cat. No. 15040066). The dissociated cells were seeded on Geltrex-coated 6-well tissue culture plates at 25,000 cells/cm2 (0.25M per well in the 6-well plates ...
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bioRxiv - Immunology 2022Quote: ... The cells were individually collected using an automated micro-manipulating system (CellCelector, ALS) and deposited in nuclease-free microtubes containing 5 μL of 2× CellsDirect buffer and RNase Inhibitor (Invitrogen). Single cell RT-qPCR was then performed using the protocol ADP41 developed by Fluidigm ...
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bioRxiv - Molecular Biology 2021Quote: ... in Minimum essential medium (MEM), glutamine-free based Neuronal growth medium (5% fetal bovine serum, 2% B27, 1% Glutamax (100×, Invitrogen), 2 % 1 mol/L d-glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μm) and a C18 analytical column (PepMapAcclaim C18, 50 cm x 075 mm, 2 μm, Dionex-Thermo-Fisher Scientific), applying a linear gradient from 2 to 35 % solvent B (80 % acetonitrile ...
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... neurons facing down, onto dishes containing the feeder layer of glia and 5 ml of culturing medium (94% Neurobasal, 2% Glutamax (Invitrogen), 2% B27 ...
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bioRxiv - Microbiology 2021Quote: Plasmodium falciparum blood stage parasites were maintained at 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 130 × g for 5 min and the pellet was resuspended in culture medium consisting of Neurobasal medium with 2% B27 (Invitrogen), 1% L-Glutamine (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at RT and were exposed to ECL imager after 5 min incubation with ECL reagent (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cancer Biology 2020Quote: Cells were sorted into 96-well plates at 50 cells/well in 5 µL CellsDirect™ 2× Reaction Buffer (Invitrogen). Cells lysates were reverse transcribed using Superscript™ III (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μm) and a C18 analytical column (PepMapAcclaim C18, 50 cm × 0.75 mm, 2 μm, both Dionex-Thermo Fisher Scientific), applying a linear gradient from 2 to 35% solvent B (80% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... 4390771 ID: s69588 and s69586) or ERK3 siRNA (5 pmol; catalog number: 4390771 ID: s78451) and Lipofectamine 2000 (2 µl) (Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated on an Acclaim PepMap 100 (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) trap column and separated on an Acclaim PepMap RSLC (75 μm × 50 cm ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were washed 2 × 5 min in PBS and mounted in ProLong® Gold antifade reagent (Life Technologies, P36934). Antibodies used for immunohistochemistry and their working concentrations are listed in Table 1.
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were permeabilised in 0.2% triton for 5 min and incubated with Click-it reaction cocktail (Click-it Alexa Fluor 647 C-10424 Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... and suspension diluted 1/5 by adding FACS staining buffer (SB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity 5 and mixed together with 2% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (A3678 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 μm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Bioengineering 2022Quote: ... 721.221 cell lines were kept in a humidified incubator at 37°C with 5% CO2 and cultured in complete RPMI1640 medium supplemented with 2 mM GlutaMAX (Invitrogen), 10% FBS (Sigma),10 mM HEPES pH 8.0 (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The inoculum was removed and cells washed 2× with PBS and 500 μL culture media (high glucose DMEM + 5% FBS (Gibco) + 1% antibiotic-antimycotic ...
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2, ATCC) were maintained in T-75 flasks (Falcon) at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 2 μL of PCR1 products were amplified by nested PCR using 5 μL of 5X Phusion HF buffer (Thermo Fisher), 0.24 μL of Phusion HF DNA polymerase (2 U/μL ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 37°C 5% CO2 in DMEM and then cells were reverse transfected with 2 μg of GFP using lipofectamine2000 (Invitrogen) in 6 well plate.
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bioRxiv - Pathology 2021Quote: ... Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm, 5 µM particles, Thermo Scientific) and separated on an analytical column (PepMap RSLC C18 ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Genetics 2022Quote: ... coli MG1693 Thy-which had been grown for 24 hours at 37C with shaking in minimal media containing 20uM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen). The culture consisted of 100 mL M9 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were harvested for flow cytometry using FACS buffer (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA) or using 0.05% Trypsin-EDTA (Thermo Fisher Scientific #25300120) for 5 min followed by quenching with medium ...
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bioRxiv - Immunology 2020Quote: ... for 2 h at 37°C and 5% CO2 in the presence of dextran-rhodamine (0.5 mg/mL) (Molecular Probes). mAb-untreated Pf sporozoites were used to measure the maximum traversal capacity ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were trapped at 20 μl/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm x 300 μm C18 pepmap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once with PBS and incubated for 2 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing 5 or 2.5 μM remdesivir alone or in combination with 10 μM ramelteon (PubChem ID 208902) ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed 3x 5 minutes in PBST and then incubated for 2 hours at room temperature with Alexa-Fluor secondary antibodies (ThermoFisher) (all 1:1000 in PBST + 5% BSA + 0.02% azide) ...
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bioRxiv - Immunology 2020Quote: ... Mast cells and macrophages were obtained from the peritoneal cavity by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). Cells were resuspended in RPMI medium containing 10% FCS.