Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
-
bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: HeLa (ATCC CCL-2) cells were grown at 37 °C and 5% CO2 in Dulbecco’s modified Eagle medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2024Quote: ... Colon was further cut into 5-10 mm pieces and put into a 15 mL falcon tube with 5 mL prewarmed 2 mM EDTA in Hank’s balanced salt solution (HBSS, Gibco) and shaken at 250 rpm for 15 min at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... comprising 2 μl sample DNA and 48 μl reaction mixture containing per reaction 5 μl PCR buffer 10x (Invitrogen), 2 μl MgCl2 (50 mM) ...
-
bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific) using a 120 minute linear gradient for separation with two FAIMS compensation voltages (-45 ...
-
bioRxiv - Synthetic Biology 2023Quote: WT HeLa (ATCC CCL-2) were cultured at 37 °C with 5% CO2 in flasks with Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 1 mM L-glutamine (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... in PBS/5% FBS at 37°C for 10 min or 2 μM H2-DCFDA (Thermo Fisher Scientific, C6827) in PBS/5% FBS at 37°C for 30 min ...
-
bioRxiv - Bioengineering 2023Quote: ... cerevisiae cells (OD 5-10) were pelleted and lysed in 200 µL 0.9% physiologic salt supplied with 2 µL RNAse cocktail (Invitrogen) and 5mg/mL Zymolyase 100T (MP Biomedicals) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2-5 µL of 50 µM siRNA stock was resuspended in 500 µL OptiMEM reduced serum media (ThermoFisher 51985034) and gently inverted to mix ...
-
bioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were adjusted to a total volume of 5 μl (2 μl solution A; 1,5 μl solution B; 0,3 μl RiboLock (40 U/μl, Thermo Scientific); 0,2 μl D-Luciferin (10 mM ...
-
bioRxiv - Microbiology 2024Quote: ... 100 µl of culture supernatant was collected and treated with 2 µl (5 U) DNase solution I (ThermoFisher Scientific) at 37°C for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 2-5 µg of the indicated plasmid using Lipofectamine 3000 as per manufacturer’s instructions (ThermoFisher). Transfection medium was exchanged to fresh medium and cells were moved to 10 cm plates 4-6 hours post-transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
-
bioRxiv - Cell Biology 2022Quote: ... coverslips were transferred to a 2 mL imaging chamber and stained with 5 μM Fura-2AM (Molecular Probes, F1221) for 30-minutes at 37°C in complete RPMI ...
-
bioRxiv - Neuroscience 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) was incubated using Click-iT™ Plus EdU Cell Proliferation Kit (Thermo Fisher Scientific) after DAPI staining ...
-
bioRxiv - Molecular Biology 2022Quote: Fresh tissue samples were washed 2–3 times in PBS and incubated in 5 U/ml dispase (ThermoFisher Scientific) supplemented with antibiotics (penicillin 50U/I and streptomycin 50 mg/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
-
bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
-
bioRxiv - Immunology 2022Quote: ... Cells then were washed twice with FACS buffer and stained for 5 minutes at 4°C with 0.5 mg/mL of 40,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher). PE and DAPI staining were measured with an iQue Screener Plus flow cytometer (Intellicyt ...
-
bioRxiv - Microbiology 2022Quote: HeLa (ATCC CCL-2) cells were grown at 37 ºC and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Genomics 2022Quote: ... using a trap column setup (2 cm length, 5 μm bead size, 75 μm inner diameter; Thermo Fisher Scientific) with a 25 cm separation column (2 μm bead size ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
-
bioRxiv - Biochemistry 2023Quote: ... coverslips were stained for 5 min with 1 μg/ml 300 nM 4′,6-diamidino-2-phenylindole (Life Technologies) to visualize nuclei ...
-
bioRxiv - Developmental Biology 2022Quote: ... followed by addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]). Then ...
-
bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]) to reduce the denaturing effect of SDc ...
-
bioRxiv - Developmental Biology 2022Quote: ... The denaturing effect of SDc was quenched by addition of 0.8 μL of 1:2500,000 diluted ERCC RNA Spike-In Mix 1 and 2.8 μL of quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]), followed by incubation at 72°C for 90 sec ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed 5 times with wash buffer and 2 times with 5x SSC (Gibco ref 15557-044) + 0,1% Tween 20 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... were maintained in a water jacketed 5 % CO2 incubator and passaged every 2-3 days using trypsin/EDTA (Gibco). 1E6 cells were seeded into 6 well plates ...
-
bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged for 5 min at 1200 rpm and washed in 1X PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
-
bioRxiv - Physiology 2024Quote: FAP proliferation was detected using 5-ethynyl-2’-deoxyuridine (EdU)/click-it assay (cat. no.: C10337 and C10340, Invitrogen). Experiments were carried out in ECM coated 96-well half-area plates ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were washed 2× with PBSTw for 5 min each and mounted in VectaShield Antifade (Fisher Scientific, Cat# NC9532821) mounting solution and stored at 4 ℃.
-
bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 5-20 nM siRNA oligos (Table 2) using Lipofecatmine RNAiMAX transfecting agent (Invitrogen; #13778-075) according to the manufacturer’s instructions for 72 hours ...
-
bioRxiv - Pathology 2024Quote: ... Cells were incubated with 5 µM Fura-2-AM in 1 mL RPMI B27 + Insulin (Thermo Fisher Scientific 17504044) at 37°C for 20 minutes ...
-
bioRxiv - Immunology 2024Quote: ... murine BMDCs at 2 × 106 cells/mL were stained with 5 μM carboxy-H2DCFDA (Invitrogen™, Carlsbad, CA, USA) and pre-incubated with prepared fatty acids (1 mM ...
-
bioRxiv - Microbiology 2024Quote: ... Cultures were maintained on blood agar base #2 (Oxoid) supplemented with 5 % (v/v) defibrinated horse blood (Thermo Scientific) and incubated under microaerobic conditions (5 % O2 ...
-
bioRxiv - Neuroscience 2024Quote: ... Time-lapse videos were recorded at 25 Hz for 5□min at 37□°C and 5% CO2 in culture media (Neurobasal media, 2% B27, 0.5□mM Glutamate, and 1% Penicillin/Streptomycin (Gibco)) ...
-
bioRxiv - Biochemistry 2024Quote: Samples were subjected to reduction and denaturation with 5 mM tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) followed by alkylation with 15 mM chloroacetamide ...
-
bioRxiv - Microbiology 2024Quote: ... synchronized iRBCs at the trophozoite stage (5-10% parasitemia, 1% hematocrit) were resuspended in binding medium (RPMI 1640 medium, 2% glucose, pH 7.2; Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... using a pre-column for sample loading (Acclaim PepMap C18, 2 cm × 0.1 mm, 5 μm, Thermo Fisher Scientific), and a C18 analytical column (Acclaim PepMap C18 ...
-
bioRxiv - Molecular Biology 2024Quote: ... using a pre-column for sample loading (Acclaim PepMap C18, 2 cm × 0.1 mm, 5 μm, Thermo Fisher Scientific), and a C18 analytical column (Acclaim PepMap C18 ...
-
bioRxiv - Molecular Biology 2024Quote: ... using a pre-column for sample loading (Acclaim PepMap C18, 2 cm × 0.1 mm, 5 μm, Thermo-Fisher Scientific), and a C18 analytical column (Acclaim PepMap C18 ...
-
bioRxiv - Bioengineering 2024Quote: ... peptides were first trapped on a trap column (PepMap 100 µm × 2 cm, 5 µm particles, Thermo Fisher Scientific) using a flow rate of 5 µL min-1 and 0.1% formic acid ...
-
bioRxiv - Cell Biology 2024Quote: ... Young (feeding) L3 larvae were collected and fed overnight with standard flyfood containing EdU (5-ethynyl-2’-deoxyuridine) (Invitrogen) and Bromophenol Blue sodium salt (Sigma) ...
-
bioRxiv - Developmental Biology 2024Quote: ... then each blastocyst was added to 5 µl of 2 × Reaction Mix (Invitrogen, CellsDirect One-Step qRT-PCR Kit) and snap-frozen on dry ice and stored at -80°C until processing.
-
bioRxiv - Developmental Biology 2024Quote: The 5-ethynyl-2’-deoxyuridine (EdU) assay was performed using a click it-488 (C10337 and Click-it, Invitrogen) by soaking the dissected CNS for 1 hr ...
-
bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (50 mM sodium phosphate, 500 mM NaCl, 20 mM imidazole, 2 mM MgCl2, 10% glycerol [vol/vol, Fisher Scientific], 0.5 mM ATP, 5 mM 2-ME, pH 7.4) supplemented with 20 µg ml-1 DNAse I ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peptides were trapped at 20 μL/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
-
bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) in 1X blocking buffer for 45 min ...