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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... passed through Xylol (2 x 5 minutes) and immediately mounted with Eukitt® (ThermoFisher).
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bioRxiv - Developmental Biology 2020Quote: ... viride were incubated in 100 μM 5-ethynyl-2’-deoxyuridine (EdU; Thermo Fisher Scientific) diluted in ASW from 24 to 48 HPA ...
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bioRxiv - Microbiology 2021Quote: ... All medium were supplemented with 2-5 or 10% fetal bovine serum (FBS; Gibco) according to the experimental setting ...
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bioRxiv - Neuroscience 2021Quote: ... and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT, #I00671G, Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... a ClickiT ® EdU (5-ethynl-2’-deoxyuridine) cell proliferation assay (Invitrogen, Paisley, UK) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... 2×10^5 individualized hESCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 protein+sgRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM DTT and 5 U/mL SUPERase.In RNase Inhibitor(Thermo Fisher Scientific #AM2696)) on ice and post-nuclear extracts placed on top of 10-50% weight/volume sucrose gradients containing the same buffer (apart from no Triton X-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The target cells were treated with 5 mg/ml MMC (#BP2531-2, Fisher Scientific) for 90 minutes at 37°C and then washed twice with RPMI medium before adding to the T cells at a 2:1 ratio (CAR T:MMC treated cells) ...
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bioRxiv - Developmental Biology 2021Quote: Fish were incubated with 0.1 mg/ml of 5-ethynl-2-deoxyuridine (Thermo Fisher) in embryo media for 2 hours prior to harvesting at 98 hpf ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 hours and 5 hours using a Floid Cell Imaging Station (Thermo Fisher Scientist), and then manually counted for quantification ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed experimental evolution using SM/5 media [56] (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Developmental Biology 2023Quote: Cell proliferation assays were conducted employing 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Cat# C10337), a thymidine analog ...
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bioRxiv - Cancer Biology 2022Quote: ... mice underwent intraperitoneal injection (IP) with 5-ethynyl-2`-deoxyuridine (EdU) (component A, Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging ...
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bioRxiv - Developmental Biology 2022Quote: ... Nuclei were stained with 5 µg / µl 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) and slides were mounted with FluoromountG (SouthernBiotech).
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bioRxiv - Cell Biology 2023Quote: ... Cy-2- or Cy-5-conjugated goat anti-mouse secondary antibodies (Thermo Fisher Scientific) were used at a 1:750 dilution in 5% HIGS/PBS ...
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bioRxiv - Biophysics 2023Quote: ... 2’(3’)-O-(2,4,6-trinitrophenyl)-adenosine 5’-triphosphate (TNP-ATP) was purchased from Invitrogen as a trisodium salt and stored in the dark at -20 °C at 10 mg/ml_ ...
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bioRxiv - Genetics 2023Quote: ... 2×10^5 individualized iPSCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 nuclease +sgRNA ...
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bioRxiv - Neuroscience 2023Quote: ... then they were incubated for 2 h in 5% normal donkey serum (NDS, Gibco), 0.2% Triton-X100 in PBS to block nonspecific binding ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in an atmosphere of 5% CO2 in an automatised incubator (Cytomat 2, Thermo Fisher). All experimental replicates were performed on a different day ...
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bioRxiv - Physiology 2024Quote: ... 25 μM Cy5-hydrazide with 10 mM 2-amino-5-methoxybenzoic acid (ThermoFisher Scientific) in 1xPBS as a catalyst was added to the cells for 15 minutes at room temperature to allow complete conjugation with the aldehyde ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were treated with 20 μM 5-ethynyl-2′-deoxyuridine (EdU, Invitrogen, A10044) for 1 hour in the presence or absence of 10 μM mtOX 53 or 6 μM DMBA (Selleck Chemicals ...
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bioRxiv - Microbiology 2024Quote: ... 2 μl of Novex Hi-Density TBE 5 x sample buffer (Thermo Fisher Scientific) was mixed with 8 μl of the EMSA reaction and loaded on an 8.0% agarose gel ...
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bioRxiv - Systems Biology 2024Quote: ... at a ratio of 2:5 into HEK293FT cell with lipofectamine 3000 (ThermoFisher, L3000008) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovaries from ∼2-5 flies were dissected in Schneider’s Drosophila media (ThermoFisher SKU #21720-001) that was also used for ex vivo culturing ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and loaded with 5 μM Rhod-2 AM (R1244, Invitrogen) in HBSS without calcium/magnesium for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached after 2 or 5 days of co-culture with StemPro Accutase (Thermofisher), experimental duplicates were performed for each timepoint and each condition ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Molecular Biology 2022Quote: ... 5 x 107 ESCs were fixed with 2 mM DSG (disuccinimidyl glutarate, Thermo Fisher Scientific) for 50 min at 25⁰C and then 1% formaldehyde (methanol-free ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Microbiology 2022Quote: Larvae were immersed in 100 μg/mL 5-ethynyl-2′-deoxyuridine (EdU) solution (A10044; Invitrogen) for 16 h before termination of the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...