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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... cells at exponential growth phase were incubated with 200 μM of the thymidine analog EdU (5-ethynyl-2′-deoxyuridine - Invitrogen) at 26 °C for 10.2 h (estimated doubling time of L ...
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bioRxiv - Microbiology 2023Quote: ... 4×105 spores and 200µl of a bacterial suspension were spread on SM/5 agar plates ( 2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Developmental Biology 2022Quote: 5-ethynyl-2-deoxyuridine (EdU) pulse labeling was performed with the Click-IT EdU imaging kit (Life Technologies; Cat#:C10340) as described in (Perillo et al. ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in assay medium (RPMI with 10% FBS and 20 IU/mL IL-2), and centrifuged (300xg, 5 mins) (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were washed in PBS with 2% Triton X-100 at 37°C for 5 days and mounted in SlowFade Diamond Antifade Mountant (S36972, Invitrogen). The stained whole mounts were imaged under the two-photon microscope.
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bioRxiv - Cell Biology 2022Quote: ... ATCC® CCL-2) were grown at 37 °C under 5% CO2 in DMEM high glucose glutamax (Gibco, 61965-059) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... and then loaded onto a PepMap C18 trap column (100 μm x 2 cm, 5 μm particle size, Thermo Scientific) followed by separation on an in-house packed column (75 mm x 19 cm ...
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bioRxiv - Neuroscience 2022Quote: ... For in vivo labelling of proliferating SOX10+ cells 5-Ethynyl-2’-deoxyuridine (EdU, 0.3 mg/ml, Thermo Fisher Scientific, E10187) was administered intraperitoneally to pregnant females two hours prior to harvesting of embryos ...
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bioRxiv - Neuroscience 2023Quote: ... To analyze the proliferative capacity of glial cells we injected 5-Ethinyl-2′-deoxyuridine (EdU, 0.05 mg/g, Thermofisher #E10187) intraperitoneally and animals were sacrificed 1hr after injection.
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Developmental Biology 2023Quote: Timed pregnant females were injected intraperitoneally with 13 μg per g body weight of 5-ethynyl-2’-deoxyuridine (Edu; Invitrogen) and sacrificed after 1 hour or 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and an Acclaim PepMap 100 C18 HPLC nanoViper Trap Column (100Å, 100 μm X 2 cm, 5 μm; ThermoFisher, USA). The tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Genomics 2023Quote: ... For the construction of the RNH2A KO clones, RNASEH2A (Chr19, exon 2) gRNA (5’-TAACAGATGGCGTAGACCAT-3’) was cloned into GeneArtTM CRISPR Nuclease Vector with OFP reporter (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Intracellular NO production was assessed during reoxygenation by live-cell staining with the NO-sensitive fluorescent dye 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein (DAF-FM) diacetate (Invitrogen). iPSC-ECs were stained with 1 μM DAF-FM diacetate for 10 minutes at 37°C ...
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bioRxiv - Genetics 2024Quote: ... NaCl and ZnCl2 for 2 hours with agitation at 800 rpm for 30s every 5 min at 37°C in thermomixer (Thermofisher), followed by addition of NH4CO3 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS) and 100 U/ml of rhIL-2 (Gibco). 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) ...
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bioRxiv - Biochemistry 2024Quote: ... 25 μl/ml BioLock biotin blocker (IBA, 2-0205-050) and 5 μl Pierce Universal Nuclease (Thermo Fisher Scientific, 88702), and kept on ice ...
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bioRxiv - Cell Biology 2024Quote: ... We removed the supernatant and gently resuspended cells with 2 ml 1X PBS containing 5 μl SUPERaseIn RNase Inhibitor (Invitrogen) and 2.5 μl RiboLock RNase Inhibitor (Thermo Scientific).
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bioRxiv - Immunology 2024Quote: Frozen PBMCs were thawed in a water bath and resuspended in 5 mL of prewarmed complete RPMI medium (RPMI 1640 [Gibco] supplemented with 10% FBS [Gibco], 2 mM L-glutamine [Gibco] ...
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bioRxiv - Pathology 2024Quote: ... Cells were incubated in a humidified incubator at 37°C with 5% CO2 in 2% DMEM (Thermo Fisher Scientific Inc) supplemented with 1% amoxicillin (Wisent Inc) ...
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bioRxiv - Microbiology 2024Quote: ... for 30 min followed by staining of host cell nuclei with 4′,6-diamidino-2-phenylindole (DAPI, 5 μg/ml final conc.; ThermoFisher) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... all wells were incubated for 5 minutes in PBS containing 0.1% 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, D1306) to counterstain nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... The cultures were maintained at 37°C in a humidified atmosphere of 5% CO2 in Neurobasal medium supplemented with 2% B27 (Invitrogen), 1% Glutamax (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 min at RT and blocked in PBS with 0.1% Triton X-100 and 2% NGS (Fisher Scientific, 11540526) for 30 minutes at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Some mice received a subcutaneous injection of 50 mg/kg 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Waltham, MA) 2 hours before euthanization ...
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bioRxiv - Neuroscience 2024Quote: Duplex of the MEF2C enhancer sequence containing a mutation site [5′-ATGTATTTTTCTGCAATAAGT-3′ (×2)] in human genomic DNA were synthesized (Invitrogen). Additionally ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 1–2 weeks of selection with fresh medium containing 200–500 μg/ml Hygromycin (Invitrogen, ant-hg-5). The surviving cells were released from the well with 0.05% Trypsin–EDTA (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µL of each sample were injected on a C18 PepMap trap column (5 µm, 300 µm I.D. x 2 cm, Thermo Scientific) at 20 µL/min ...
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bioRxiv - Biophysics 2024Quote: C2C12 cells were magnetically labelled by an incubation with a solution of iron oxide nanoparticles at [Fe] = 1 - 2 mM and 5 mM citrate in RPMI 1640 medium (Gibco) for 30 minutes leading to an uptake of about 3 pg of iron per cell (measured by magnetophoresis) ...
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bioRxiv - Bioengineering 2024Quote: PAMAM dendrimer G5 (5 mg in 0.05 ml dimethylformamide) was biotinylated with 2 mol eq of NHS-PEG4-biotin (ThermoFisher, USA) for 6 h and then primary amines were blocked with 150 mol eq of glycidol for 18 h ...
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bioRxiv - Cell Biology 2024Quote: HEK293T (CRL-3216) and HeLa (CCL-2) were purchased from ATCC and cultivated at 37°C and 5% CO2 in DMEM (Gibco) complemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were transfected with 25 nM siRNAs (siControl, siMICAL1, siMICAL1#2, siRab35) for 5 days using Lipofectamine RNAiMAX (Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... or 5-TAMRA (5-Carboxytetramethylrhodamine) (Thermo Scientific) in 0.1M Na2CO3 (pH 9.0 ...
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bioRxiv - Biophysics 2021Quote: 106 T-cells were incubated in T-cell media supplemented with 5 µg ml-1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and HER-2 negative 24JK sarcoma cells were labelled with 5 mM or 0.5 mM CellTrace™ Violet (CTV; Invitrogen-Molecular Probes) respectively in D-PBS supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... and then incubated overnight at 4°C in 2 ml TBST/5% milk with a 1:1,000 dilution of anti-CPY Monoclonal antibody 10A5B5 (Invitrogen, A-6428). The nitrocellulose was then washed 3 times in 20 ml TBST at room temperature for 10 min ...
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...