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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Fixation for 5 min at room temperature was performed by adding an equal volume of PBS 2% methanol-free formaldehyde (Thermo Scientific, USA ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Genetics 2021Quote: ... and primers specific for target and control regions (in which the mark is expected to be enriched (positive controls) or depleted (negative controls)) (Table 2) using the QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty million cells were diluted in 2 mL of GBSS containing 1% FBS and stained with 5 μg/mL of Hoechst 33342 (Invitrogen H3570) for 1 h at 32 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were subject to local UVC irradiation at 500 J/m2 and incubated with 20 μM 5-ethynyl-2-deoxyuridine (EdU, ThermoFisher Scientific) and 1 μM 5-Fluoro-2’-deoxyuridine (FUdR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Differentiation was stimulated by replacing the proliferation medium with differentiation medium (high glucose DMEM with sodium pyruvate, 5% horse serum, 2% L-glutamine, ThermoFisher Scientific). The cells were maintained at 37°C and 5% CO2 throughout differentiation.
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...
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bioRxiv - Genomics 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 μm × 50 cm ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Biophysics 2020Quote: 106 T-cells were incubated in T-cell media supplemented with 5 μg ml−1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Biochemistry 2020Quote: ... targeting the TLR3 gene (5’-CCUGAUGAUCUUCCCUCUAACAUAA-3’) and Stealth RNAi siRNA negative control med GC Duplex #2 were obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were loaded on to 2cm trap column (C18, 5 μm, 100 Å, 100 μm × 2 cm; PN: 164562, Thermo Scientific) and further loaded on 50 cm Easynano spray analytical column (2 μm ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA at a concentration of 1-2 mg/ml was mixed with 5 µl of Salmon Sperm DNA (10mg/ml) (Thermofisher Scientific) prior to electroporation ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos or ovaries dissected from 5 well-fed females per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μl TURBO DNase (2 U/μl ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap C18, 5 μm, 100 μm × 2 cm, Thermo Fisher Scientific) connected to an analytical column (Acclaim PepMan 100 ...
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bioRxiv - Plant Biology 2020Quote: ... and a final extension of 5 min at 72°C. The target band (approx. 100 bp) was extracted from a 2% E-Gel (Thermo Fisher). For multiplex sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then stained with DAPI (2 ug/mL) for 5 minutes before mounting with ProLong Diamond antifade mountant (Thermo Fisher).
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bioRxiv - Systems Biology 2020Quote: ... Peptides were trapped at 20 μl/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm x 300 μm C18 pepmap cartridge pre-column (Thermo Fisher Scientific) during 5 minutes ...
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bioRxiv - Microbiology 2020Quote: HeLa cells (ATCC CCL-2) were incubated in RPMI (Roswell Park Memorial Institute) medium containing 5% FCS (fetal calf serum, Gibco®) in an incubator with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... digests were reconstituted in 10 μL of 0.3% TFA in 2/98 MeCN/H2O and 5 μL were analyzed by LC-MS/MS using an RSLCnano system (Ultimate 3000, Thermo Scientific) interfaced on-line to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Pregnant female mice received intraperitoneal (i.p.) injections with 50µg of 5-ethynyl-2’-deoxyuridine (EdU)/g body weight (Thermo Fisher Scientific – A10044), 16 hours prior to tissue collection ...
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bioRxiv - Microbiology 2020Quote: ... and purified RNA from 2 ml of cells harvested immediately before and 5 min after stress using the RiboPure™-Bacteria Kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was removed from the antibody by boiling at 100 °C for 5 min in 2 X LDS Sample buffer (Life Technologies). An equal volume of each sample and its corresponding input was loaded onto a gel for electrophoresis and western blot analysis.
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bioRxiv - Cell Biology 2021Quote: ... GFP-associated or immunoprecipitated complexes were eluted (30 μl) at pH 2 for 5 min at 25°C and neutralized according to the manufacturer’s instructions (88805; Thermo Fisher Scientific). Samples were then reduced at 70°C for 5 min by dilution in 5x sample buffer (125 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... incubation with secondary antibody was performed in 0.5% BSA/PBS for 1 hour at room temperature and nuclei were stained with 2 μg/ml Hoechst 33342 (Life Technologies). Images were acquired using the Leica SP8 confocal or Leica DMI6000-SD microscopes ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... blotted for 5 s using blot force 2 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). The data were collected on a Thermo Scientific™ Krios™ G4 Cryo Transmission Electron Microscope (Cryo-TEM ...
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bioRxiv - Molecular Biology 2022Quote: Visualisation of Fasciola proliferative cells from 21 day old juveniles maintained in vitro and in vivo was achieved by labelling nuclei undergoing DNA-synthesis with 5-ethynyl-2-deoxyuridine (EdU; ThermoFisher Scientific) as described by [15] ...
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bioRxiv - Cell Biology 2022Quote: ... cells were passaged at day 5 of differentiation into 2-well chamber slides for the remainder of hepatic directed differentiation (ThermoFisher Scientific). For mitochondrial morphology analysis MitoTracker Deep Red FM (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and a goat anti-mouse antibody conjugated with Alexa Fluor 488 (Life Technologies; 2 μg/ml in ND96 with 5% BSA) was applied for 1h at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU pulse-labelling was performed at the indicated time points by 30 min incubation in the presence of 10 µM 5-ethynyl-2-deoxyuridine (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: Cells were set at a concentration of 1 x105 cells/mL 24 hrs prior to EdU addition (5-ethynl-2’-deoxyuridine; Life Technology, Thermo Scientific) in media free from thymidine prepared using Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were enzymatically dissociated at 37°C for 2 × 20min in the HBSS solution supplemented with calcium (CaCl2 5 mM) and containing collagenase type II (Gibco, France) and dispase (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed in 0.1 M TB (2 ×5 minutes) and counterstained with Hoechst 33342 (1:20000, #62249, Thermo Fisher Scientific) diluted in 0.1 M TB ...
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bioRxiv - Cancer Biology 2021Quote: ... To visualize sister chromatid exchanges (SCE) slides were rehydrated in PBS for 5 min and stained with 2 μg/mL Hoechst 33342 (Thermo Fisher) in 2xSSC (final concentration 300 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
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bioRxiv - Microbiology 2022Quote: ... Amino acids were extracted on ice-cold lysis buffer [5:3:2 ratio of methanol-acetonitrile-water (Fisher Scientific, Pittsburgh, PA)] containing 3 µM of amino acid standards [Cambridge Isotope Laboratories ...
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bioRxiv - Immunology 2022Quote: ... 30 μl of SARS-CoV-2 S-5P or 6P at 5 ng/µl were plated onto 384-well Nunc Maxisorp (Thermo Fisher) plates in PBS and sealed overnight at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Ly6Chi monocytes were labeled by intraperitoneal injection of 4 mg/mL of Edu (5-ethynyl-2’-deoxyuridine) from Life technologies (NY), and mice were euthanized after 5 days to assess baseline recruitment or 21 days to assess for retention ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Scientific) and separated on an in-house packed analytical column (75 μm x 35 cm ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...