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Citations for Cell Signaling Technology :
8651 - 8700 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Cells were rinsed with pre-warmed 1x PBS and fixed with 1x fixative solution provided by senescence beta-galactosidase staining kit (9860, Cell Signaling Technology) for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: Hif2α and ARNT chromatin immunoprecipitation (ChIP) assays were performed using a SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology #56383) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: CUT&RUN assay was conducted following our prior protocol[51] using 200,000 MuSCs or IMR-90 cells with the CUT&RUN assay kit (Cell Signaling Technology, 86652). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunocapture of GG-modified peptides was then performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Five-micron paraffin-embedded sections were labeled with primary antibodies against phosphor-p44/p42 MAPK (Erk1/2) (Thr 202/Tyr 204) (Cell Signaling Technology Cat# 4370, 1:200, Danvers, MA, USA), Ki-67 (ab15580 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-PSA/KLK3 (D6B1) PARP (9542) and cell cycle regulation sampler kits (9932 and 9870) were obtained from Cell signaling technologies (Danvers, MA). The secondary antibodies (IR dye 680 and IR dye 800 ...
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bioRxiv - Cancer Biology 2021Quote: ... or of 35 mg (for mouse tumors) total proteins using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology), according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Cells were treated with doxycycline for 7 days and were fix and stained with a Senescence β-Galactosidase Staining Kit (Cell Signaling, 9860) according the manufacture’s instruction and incubated at 37 °C for overnight.
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bioRxiv - Cancer Biology 2019Quote: ... 25 mg tumour tissue / IP were fixed in 1% formaldehyde (Applichem) and processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Cancer Biology 2020Quote: ... 90% of the pre-cleared lysates were subsequently added to prewashed glutathione agarose beads from Active Ras Detection kit (Cell signaling Technology, #8821) for 1 hour at 4 °C under constant rocking ...
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bioRxiv - Neuroscience 2021Quote: SA-β-gal staining was performed according to the protocol of the Senescence-β-Galactosidase Staining Kit (Cell Signaling Technology, Danvers, MA, USA). At least 200 cells were counted in each treatment group from three independent experiments.
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellet was then resuspended in 1 X PBS and cell fractionation was carried out using the Cell Fractionation Kit (Cell Signaling Technology, 9038) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... The proportion of senescent cells was determined by staining for β-galactosidase activity using Senescence β-Galactosidase Staining Kit (9860, Cell Signaling Technology) as described42.
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... we analyzed acetylation levels of Histone H3 in HDACI treated cells using the PathScan® Acetylated Histone H3 Sandwich ELISA Kit (Cell Signaling Technology) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... and fixed and stained for Senescence Associated β-Galactosidase Activity (SA-β-Gal) using the for Senescence β-Galactosidase Staining Kit (Cell Signaling) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... cells were stained with senescence-associated (SA)-β-galactosidase (gal) according to the manufacturer’s instructions for Senescence β-Galactosidase Staining Kit (Cell signaling, 9860). Cells were counterstained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: SUM159 and MDA-MB-231 cells were stained for SA-β-gal detection using a Senescence β-galactosidase Staining Kit (#9860, Cell Signaling). Staining was performed by following the manufacturer’s instructions and incubating the stained samples for 48hrs at 37°C without CO2 ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen sections were hydrated with PBS for 10min at RT and stained using Senescence β-Galactosidase staining kit according to manufacturer’s instructions (Cell signaling technology, 9860). The sections were incubated at 37℃ in a dry incubator (no CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cellular lysates from FLSs were harvested and a ChIP assay was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). In brief ...
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bioRxiv - Cancer Biology 2023Quote: Cleaved PARP as an apoptotic marker in cell lysates was measured using PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit (Cell Signaling Technology) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were dried by lyophilization and resuspended in IAP buffer from PTMScan HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). K-GG containing heavy reference peptides were pooled and diluted into the IAP buffer resuspension fat a final concentration of 1 fmol per peptide ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were incubated with primary antibodies (PHOX2B: 1:1000, ab183741, abcam; n-Myc: 1:500, 51705 Cell Signaling Technology; CD45: 1:1000, 70257 Cell Signaling Technology; CD3: 1:2000 A0452 Dako/Agilent) diluted in TBST+5% goat serum in a humid chamber overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and myotube (4 days differentiation in low serum differentiation medium) and ChIP was performed using the Simple ChIP Enzymatic Chromatin IP Kit (Cat# 9003; Cell Signaling Technology; Danvers, MA) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2021Quote: Approximately 1.2 million microglia were exposed to the indicated treatments followed by cell preparation according to the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003). Samples were cross-linked for 10 min at room temperature with 1% formaldehyde solution ...
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bioRxiv - Cell Biology 2022Quote: ... PathScan® Phospho-Akt2 (Ser474) and Total Akt2 Sandwich ELISA kits were used to quantify AKT2 levels (Cell Signaling Technology #7048 and #7046 respectively) as per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The PathScan® Phospho-S6Kinase (Thr389) Sandwich ELISA and PathScan® Total S6K Sandwich ELISA Kits were purchased from Cell Signaling Technologies (Danvers, MA). 200,000 FL83B cells/well were seeded onto a 24 well plates and allowed to grow for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Specificity of C12FDG detecting senescent cells was determined in comparison with results obtained with the senescence β-galactosidase staining kit (Cell Signaling Technology #9860) on IMR90 cells that were treated with 100 ng/mL doxorubicin (Sigma ...
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bioRxiv - Genetics 2019Quote: ChIP followed by allele-specific quantitative PCR (ChIP AS-qPCR) [38] were performed in U2OS cell line by using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalog# 9003) (Cell Signaling Technology, USA) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The following steps were performed according to the manual of the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). In brief ...
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bioRxiv - Cell Biology 2023Quote: SA-β-gal staining was performed using a SA-β-gal staining kit according to the manufacturer’s protocol (Cell Signaling Technology, Danvers, MA, USA). In short ...
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bioRxiv - Cancer Biology 2023Quote: IMR90 cells seeded on the plate were fixed and stained for SA-β-gal activity with the senescence β-Galactosidase Staining Kit according to the protocol (Cell Signaling, #9860S).
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Cell Biology 2024Quote: ... that were treated with PBS or Dox or transduced with adenovirus harboring shRNA scramble (shCtrl) or shRNA Atg7 (shAtg7) were stained with the SA-β-gal staining kit (Cell Signaling Technologies, #9860), according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest was split into 8 aliquots dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784) as described in the manual and peptides were eluted twice with 200µL of 50% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2024Quote: RAS activity was assessed by affinity pull-down of endogenous levels of GTP-bound Active-Ras as described by Active Ras detection kit (Cell Signaling Technology, cat# 8821).
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005; GRK6: Cell signaling technology #5878, Actin: Sigma-Aldrich A5441). For experiments using the MOP phospho-specific antibodies see below ...
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bioRxiv - Microbiology 2019Quote: ... 1:10,000 dilution]) and then with secondary antibody (horseradish peroxidase–conjugated anti-rabbit IgG [Cell Signaling Technologies, 7074S] or anti-mouse IgG [Cell Signaling Technologies, 7076S], both at a 1:5,000 dilution]).
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585, 1:1000, overnight at 4°C / 24E10, Cell Signaling Technology, #3195, 1:1000, overnight at 4°C) and horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000 ...
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bioRxiv - Genomics 2024Quote: ... dissolved in in 1x TBS with 1% Tween-20 (TBS-T) for 60 min and separately incubated with primary antibodies: anti-HA (Cell Signaling Technology 2367S, 1:2000 dilution in 5% BSA/TBS-T) and anti-beta-tubulin (Invitrogen 32-2600 ...
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bioRxiv - Cancer Biology 2020Quote: Cells treated for 48 h with NaB were lysed with a lysis solution buffer and acetylation of H3 was measured with PathScan® Acetylated Histone H3 Sandwich enzyme-linked immunosorbent assay (ELISA) Kit (Cell Signaling Technology, Danvers, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). For chromatin preparation from tissue ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...