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Citations for Cell Signaling Technology :
8501 - 8550 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Tubulin (Cat# 2148), ATG5 (Cat#12994), Actin (Cat # 8456) and anti-p62/SQSTM11(Cat# 5114, #7695) antibodies were purchased from Cell Signaling Technology (Danvers, MA 01923, USA). The mouse anti-FLAG (Cat# TA50011 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight incubation with the primary antibodies (LC3B Cell Signaling 3868S rabbit polyclonal, 1:300; or F4/80 Cell Signaling 70076S rabbit monoclonal, 1:200). Next day ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed four times for 5 min each and treated with 1:1000 diluted Alexa Fluor® conjugated sec antibody (Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were then incubated at 4°C overnight in Odyssey Blocking buffer containing 0.1% Tween 20 and the following antibodies: ERK 1/2 (mouse monoclonal, Cell Signaling Technology no. 4696; 1:1000 dilution), pERK 1/2 (rabbit monoclonal ...
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bioRxiv - Immunology 2020Quote: ... at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich, Oakville, Ontario, and Anti-rabbit HRP-linked Antibody (7074S - Cell Signaling Technology, Danvers, MA) at 1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ERK-type MAP kinases (P~Kss1p, P~Fus3p and P~Slt2p) were detected using α-p44/42 antibodies (Cell Signaling Technology, Danvers, MA, 4370 and 9101) at a 1:5,000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated, Cell Signaling, Cat #: 7076, 1:5,000 dilution) prepared in the WB blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... #2639), anti-GAPDH (clone 14C10, #2118), rabbit anti-HA-tag (clone C29F4, #3724) antibodies for immunoblots were purchased from Cell Signaling Technology (Frankfurt am Main, Germany). Mouse anti-β-actin (clone AC-15 ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... Protein expression of RIG-I mutants in HEK Flp-In™ T-REx™ 293 cells was compared by immunoblot with wild-type RIG-I using an antibody against RIG-I (Cell signaling, Frankfurt am Main, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... CD8 (ab4055), CD4 (EPR6855, ab133616) at a 1:500 dilution and PD-L1 (rabbit monoclonal anit-PD-L1 primary antibody; Cell Signaling Technology, clone: E1L3N, CAT#13684) at a 1:200 dilution on the Leica BOND RX ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2024Quote: ... LC3A/B XP Rabbit mAb (Cat. NO. 12741S; clone name, D3U4C) and β-actin antibody (Cat. NO. 4967) were from Cell Signaling Technology (Danvers, MA, USA). EpCAM monoclonal antibody ...
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bioRxiv - Microbiology 2024Quote: ... blocked in 3% goat serum for 1 hour at room temperature and incubated in mouse anti-Sag1(28) [DG52] and anti-NF-κB (Cell Signaling Technology, 8242S, 1:1000 antibodies) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was incubated with primary antibodies (rabbit anti-EGFR D38B1 Cell Signaling Technologies, rabbit anti-HER2 2242 Cell Signaling Technologies, rabbit anti-PD-L1 E1L3N Cell Signaling Technologies, rabbit anti-CTAL4 E1V6T Cell Signaling Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then reacted with a secondary antibody (Anti-mouse IgG, and Anti-rabbit IgG conjugated to Horseradish Peroxidase (both from Cell signaling, Cat # 7076 and # 7074, respectively), developed using Western BrightTM enhanced chemiluminescence reagent (Advansta) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% Tween-20)) and then incubated with primary antibodies (PC: Proteintech, 16588-1-AP, 1:1000 dilution, or β-actin: Cell Signaling, 8457S, 1:1000 dilution) in TBST supplemented with 5% BSA at 4°C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were transferred onto nitrocellulose membranes for immunoblotting and then probed with antibodies for phospho-AMPKα (Thr172) and β-Actin (Cell Signaling Technology, Danvers, MA, USA). The membrane was probed with appropriate secondary antibodies and imaged on an Odyssey Imaging System (LICOR ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 minutes in TBS-T and incubated overnight in primary antibodies against phosphorylated Akt T308 (Cell Signaling Technologies #2965; diluted 1:1000), phosphorylated Akt S473 (Cell Signaling Technologies #9271 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195; Fibronectin, 1:1000, Cell Signaling Technology, #26836 ...
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bioRxiv - Developmental Biology 2023Quote: ... portions of the entire pancreas were sectioned at 5μm thickness and ≥3 non-consecutive sections of each portion were stained with an antibody against insulin (1:100, Cell Signaling Technologies: 4590, Danvers, MA, USA), which was subsequently detected by biotinylated goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were carried out on the remaining sample overnight at 4 °C with rotation with 5 uL of antibody (Cell Signaling Technology D31H2 anti-CTCF, 3418S). 50uL of Protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Bead-bound protein was extracted using the SDS loading buffer and analyzed by SDS-PAGE and western blotting using anti-HA (Primary) antibody (Cell Signaling, Cat#3724S, 1:3000 dilution) and anti-rabbit IgG-HRP (Secondary ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated with primary antibody (H3K4me3, Active motif #39160; H3K27me3, Cell Signaling Technology 9733 C36B11; H2AK119ub, Cell Signaling Technology 8240s; H3K9me3, active motif #39161) at a ratio of 1:100 at 4°C for 2 h on a thermomixer at 400 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Neuroscience 2024Quote: Method and general reagents for Western blots were described previously with a few modifications [10] Adipor1 and Tubulin antibodies utilized were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and rabbit anti-α Tubulin (Cell signaling, Catalog No. 2125, MA), respectively.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
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bioRxiv - Immunology 2021Quote: SA-β-gal activity was detected in IMR-90 cells using a SA-β-gal staining kit (Cell Signaling, #9860). IMR-90 cells (40,000 cells/well in a 12-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... The BrdU assay was performed with the BD Accuri C6 flow cytometer using the APC-BrdU flow kit according to the manufacturer’s instructions (Cell Signaling).
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bioRxiv - Cell Biology 2020Quote: Cryo-embedded sections were stained using the senescence associated β-galactosidase (SA-β-Gal) staining kit (Cell Signaling Technology, 9860) as per the manufacturer’s instructions with the following modifications for tissue sections ...
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bioRxiv - Cancer Biology 2021Quote: ... RAS-GTP pulldown was performed according to the manufacturers’ instructions using the Active Ras Detection Kit (Cell Signaling Technology, #8821). In brief ...
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bioRxiv - Genetics 2022Quote: ... Chromatin from crosslinked cells was fractionated by digestion with micrococcal nuclease using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) according to the manufacturer protocol ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... lysed and active RAS levels were measured by affinity purification using an Active Ras Detection Kit (Cell Signaling Technologies, 8821S). Pulldown samples were loaded on a 4-12% Bis-Tris NuPage Protein gel (NuPage ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular cAMP concentrations were determined using a cyclic AMP XP Assay Kit (Cell Signaling Technology, catalog number 4339S, Danvers, MA) using 50μg total protein per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... K-□-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-□-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described62 ...
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bioRxiv - Cancer Biology 2020Quote: Senescence-associated-β-galactosidase (SA--β-gal) staining was performed using the Senescence β-Galactosidase Staining Kit (Cell Signaling, #9860) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: We subjected freshly fixed samples to β-galactosidase (β-gal) staining by using either β-gal staining kit (Cell Signaling), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Senescence was assessed using the Senescence β-Galactosidase Staining Kit at pH 6 following the manufacturer’s instructions (Cell Signaling Technology). For SAHF staining ...
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bioRxiv - Molecular Biology 2023Quote: ... Senescence-associated (SA) β-galactosidase activity was determined by using the Senescence SA-β-Galactosidase Staining Kit (Cell Signaling, #9860), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... DiGly peptide enrichment was performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cell Biology 2022Quote: S2R+ cells expressing Flag-dwg were subjected to ChIP assays using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ChIP-seq analysis from P35-42 mouse cortex was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003) following manufacturer’s instructions with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Fixation and staining were performed using a β-galactosidase staining kit per the manufacturer’s instructions at pH 6.0 (Cell Signaling). Cells were counterstained with DAPI to enable quantification.
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bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
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bioRxiv - Biochemistry 2023Quote: ... cGMP was measured in duplicate according to manufacturer’s directions using the Cyclic GMP XP® Assay Kit (Cell Signaling #4360) after the supernatant was diluted 1:10 in 1× Cell Lysis Buffer (Cell Signaling #9803) ...