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Citations for Cell Signaling Technology :
8401 - 8450 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed 3 times for 10 minutes in TBS-T buffer and then incubated in secondary antibodies made against mouse and rabbit as appropriate in milk at a dilution of 1:4000 (Cell Signaling Technologies, Danvers, MA, USA). Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then washed three times for 5 minutes each with 1X TBST and incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling, Cat # 7074S, 1:2000) in 5% BSA for two hours at room temperature with gentle rocking ...
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bioRxiv - Physiology 2021Quote: ... Phospho-p44/42 MAPK (Thr202/Tyr204 pERK1/2; 9101S) and p44/42 MAPK (ERK1/2; 9102S) antibodies were from Cell Signaling Technologies (Danvers, MA, USA). HRP-conjugated goat anti-rabbit (170–6516 ...
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated in rabbit anti-phospho STAT5 antibody (dilution 1:1000, polyclonal rabbit anti-phospho-STAT5, Tyr 694, #9351, Cell signaling Technology, Beverly, MA, USA) for 48 hours ...
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bioRxiv - Plant Biology 2021Quote: ... MPK phosphorylation was determined in protein extracts obtained from elicitor or control-treated leaf disks using immunoblots probed with antibodies against phosphor-p44/42 ERK (Cell Signaling Technology, Beverly, MA, USA) and horseradish peroxidase (HRP)-labeled secondary antibodies for luminescence detection (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were then blocked with 5% nonfat milk at room temperature for 1 hour and then probed with primary antibodies (phospho-p44/42 MAPK, Cell Signaling 9101, 1:4000, dilution) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... IHC staining with primary antibodies against LC3-I/II (D3U4C) (#12741, 1:2000), Beclin-1 (D40C5) (#3495, 1:400) (Cell Signaling Technology, Danvers, MA, USA) and Ki67 (SP6 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence staining was performed as described previously (Yuan et al., 2020) with primary antibodies to c-Jun (1:1000, Cell Signaling Technology, Danvers, MA, USA) and c-Fos (1:1000 ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was solubilized in RIPA buffer and then subject to SDS-PAGE and immunoblotting with antibodies to total SRC and phospo-Y416 SRC (Cell Signaling, 2110 and 6943 respectively). Membranes were scanned using a Licor Odyssey fluorescent imaging instrument and densitometry conducted using Image Studio Lite ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated in secondary antibody (HRP conjugated anti-rabbit or anti-mouse IgG(H+L) (1:10,000 dilution, Cell Signaling, cat # 7074P2 or 32230, respectively). Subsequently the signal was developed using Super Signal West Femto substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were blocked with non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: anti-P2RX4 (Cell Signaling Technology, Cat# 70659, RRID:AB_2799789) and anti-P2RX7 (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... Membrane was blocked using 1x Roti®Block (Carl Roth) for one hour and incubated over night at 4 °C with the primary antibodies against cGAS (Cell Signaling Technology, #31659, 1:1000), β-actin (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed again 4 times in TBST for 5 m each and blocked again before incubating with primary antibodies: total Akt (1:1000, Cell Signaling Technology, Cat. #9272S, RRID:AB_329827) & total S6RP (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was incubated for 1 h with 5% bovine serum albumin in 1 x TBS/0.1% Tween (TBST) before being incubated with primary antibodies: pAkt (1:2000, Cell Signaling Technology, Cat. #4060, RRID: AB_2315049), and pS6RP (1:2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Microbiology 2020Quote: ... Immunodetection was carried out by using rabbit anti-γ-H2A.X (SPQ) primary antibody (generated using peptide containing the SPQ motif, Cell signaling cat # 9718S, 1:1000), anti-H2A antibody (Abcam cat# ab88770 ...
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bioRxiv - Bioengineering 2019Quote: ... 1:50, Sigma-Aldrich, cat. nr. HPA050007; goat αV5 antibody, 1:350, Abcam cat. nr. ab95038; and, rabbit αHA, 1:800, Cell Signaling cat. nr. C29F4) at 4°C overnight ...
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bioRxiv - Physiology 2020Quote: ... China) at room temperature for 1 h before incubation with a mouse anti-human CD44 monoclonal antibody (Cat. #3570; Cell Signaling Technology, Danvers, MA, USA) and a rabbit anti-human CD24 antibody (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane was washed three times with X1 TBST for 10 min each and incubated with HRP□conjugated rabbit or mouse secondary antibodies (#7074 and #7076, Cell Signaling Technology Inc., Danvers, MA) at 1:5000 dilution in X1 TBST with 5% nonfat dry milk for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were blocked with 10% goat serum plus 1% BSA at room temperature for 30 minutes and then incubated with the primary antibody against Ki67 (Cell signaling, Cat #12202S, 1:800) or c-Myc (Cell signaling ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204; #4370 from Cell Signaling Technology, Danvers, MA, USA). A mouse anti-α-tubulin antibody (#T9026 from Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505; anti-HA, Cell Signaling Technology, #3724S ...
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bioRxiv - Bioengineering 2022Quote: ... Cells on coverslips were incubated with diluted primary antibodies in PBST against myosin light chain-2 (MLC-2, Cell Signaling Technology, 3672S, Rabbit, 1:100), RAR-β (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotting was performed as described above and biotinylated (internalized) β1-integrin was detected by immunoblotting with horseradish peroxidase (HRP)-conjugated anti-biotin antibody (Cell Signaling Technology, 7075, 1:15000) and imaging with the chemiluminescence film-based system ...
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bioRxiv - Biochemistry 2019Quote: ... and phosphorylated serines were detected with PKA phosphosubstrate antibody directed against the consensus site RRX p(S/T) (Cell Signaling Technology, 100G7E, rabbit mAB #9624) in blocking buffer overnight at 4°C(Christian et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were transferred to a PVDF membrane and blocked for one hour in 5% dry milk before incubation with KIF3A (D7G3) rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, #8507) in TBS containing 0.1% Tween-20 and 5% bovine serum albumin (BSA ...
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Mitochondrial ROS1 increases mitochondrial fission and respiration in oral squamous cancer carcinomabioRxiv - Cancer Biology 2020Quote: ... 1:1000 for immunoblotting, 1:100 for immunofluorescence staining) and anti-DRP1 (#8570, 1:1000 for immunoblotting) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH (#G8795 ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunoblots were probed with different antibodies: #3371 anti-pERK5 (only works for immunoblotting after immunoprecipitation) and #3372 anti-ERK5 from Cell Signaling Technology (Danvers, MA, USA); clone 07-507 anti-pERK5 (Millipore) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1:1000) and Santa Cruz Biotechnology (Actin-HRP, #sc-47778 HRP, 1:5,000) and secondary HRP-conjugated antibodies from Cell Signaling (#7074 and #7076, 1:10,000). HRP was detected using ECL substrate purchased from Thermo Scientific (#34076 ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemical stainings of pERK1/2 and pS6 were performed on 5 μm thick coronal sections of paraffin-embedded mouse brains using primary antibodies raised against pERK1/2 (1:300, Cell Signaling Technology Cat# 4370, RRID:AB_2315112) and pS6 (1:300 ...
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bioRxiv - Biophysics 2020Quote: ... M-phase was discriminated with the phospho-Histone H3 (Ser-10) primary antibody (1:25 in PBS with 1% of NGS, Cell Signaling Technology, USA [RRID:AB 331748]) and the secondary anti-mouse IgG antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... 2% normal goat serum and 0.2% Triton-X100 in PBS) for 30 minutes and hybridized with polyclonal rabbit anti-phospho-Histone H3 (Ser10) antibody (Cell Signaling Technology, #9701; 1:1000) at overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... incubated with primary antibody (mouse anti-HK2, Novus Biologicals, #NBP2-02272; rabbit anti-PARP1/cPARP1, Cell Signaling Technology, #9542; rabbit anti-Caspase3/cCaspase3, Cell Signaling Technology, #9662 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue sections of GFP+ tendon cells in each group were observed through a fluorescent microscope and confirmed by immunostaining with rabbit anti-GFP primary antibody (Cat # 2956S, Cell Signaling Technology Inc, Danvers, MA), followed by Cy3-conjugated goat anti-rabbit IgG as the secondary antibody (Cat # AP132C ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck, or #14793 Cell signaling, Leiden, the Netherlands), for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x; ERG, Cell Signaling Technologies # 97249). Subsequent chromatin capture ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were incubated overnight at 4°C in a primary antibody solution consisting of rabbit-anti-pSMAD1/5/9 (1:600, Cell Signaling Technology #13820 Lot 3) and goat-anti-SOX2 (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... stored as free-floating sections in phosphate buffered saline at 4°C and then incubated with a rabbit antibody against cFos (1:1000, Cell signaling #2250, Danvers, MA, USA) for 48 h ...
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bioRxiv - Pathology 2022Quote: ... 4 µm sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using an anti-nucleocapsid rabbit monoclonal antibody (HL344, Cell Signaling Technology, Danvers, Massachusetts). IHC was performed using the automated BOND-RXm platform and the Polymer Refine Red Detection kit (Leica Biosystems ...
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bioRxiv - Systems Biology 2022Quote: ... an Odyssey blocking buffer solution containing 10% OptiPrep™ and 1:2000 Alexa 488-conjugated anti-phospho-histone H3 (S10) antibody (pH3) (clone D2C8, Cell Signaling Technology, Danvers, MA) to label M-phase cells and 2 µg/ml Hoechst 33342 to stain all nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies (PGRN R&D Systems af2557, 1: 400; GPNMB Abcam, ab188222 1:2500; MITF Cell Signaling Technologies 97800S, 1:1000; H3 Cell Signaling Technologies 9715S ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Cancer Biology 2024Quote: ... sonicated chromatin was supplemented to final concentration of 1.1% Triton-X and 5% glycerol and subsequently set up for immunoprecipitation with the following antibodies: Rabbit Anti-POU2F3 (Cell Signaling E5N2D, #36135, 1:50), mouse anti-Rpb1 (Cell Signaling 4H8 ...