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Citations for Cell Signaling Technology :
8201 - 8250 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: SA-β-gal activity was determined on coronal brain sections using a SA-β-gal kit (Cell Signaling) according to the manufacturer’s instructions ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ChIP assay was conducted using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, Danvers, Massachusetts, USA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Chromatin samples for ChIP‒qPCR were prepared using a SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, 9003) according to the manufacturer’s instructions ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: CIC-DUX4 immunoprecipitation was performed with NCC_CDS_X1 cells using the SimpleCHIP Enzymatic Chromatin IP Kit (Cell Signaling Technology) with IgG (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... The ChIP assay was performed using a Simple Chip Enzymatic Chromatin IP Kit (Cell Signaling Technology, cat# 9003) as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were washed and incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:3000, Cell Signaling Technology, #7076 or #7074) and followed by detection with ECL Western blot substrate (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were performed as described previously (Ji et al., 2019; He et al. 2021) using antibodies against β-catenin (Cell Signaling, Cat#9581), WDR77 (SCBT ...
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bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen detection was performed by overnight incubation with appropriate antibodies: 1:1000 rabbit monoclonal Plexin-A4 (catalog #C5D1) anti-mouse (Cell Signaling Technology, catalog #3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6 ...
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bioRxiv - Neuroscience 2021Quote: ... Sonicated lysates were cleared once by centrifugation and incubated overnight at 4°C with magnetic beads bound with NFκB antibody (Cell Signaling Technology, Cat. No. 8242s) to enrich for DNA fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were blocked with 3% BSA in PBS-T for 30 min and then incubated for 1 h with the following primary antibodies (diluted in 1% BSA in PBS-T): anti-RPA (Cell Signaling, #2208, 1:200), anti-Rad51 (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked in 4% FBS in 1x PBS + 0.1% Triton X-100 prior to incubation with primary antibodies diluted in blocking buffer: rabbit anti-γ-H2AX (Cell Signaling, 2577, 1:400); mouse anti-p53 (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... and subjected to immunoblotting with the antibodies for phosphorylated Erk-1/2 (Thr202/Tyr 204) or total Erk-1/2 from Cell Signaling Technology (Beverly, MA). Blots were visualized by enhanced chemiluminescence western detection system (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... for 60 min, the membrane was washed once with TBST and incubated with antibodies against DUSP3 (4752, Cell Signaling Technology β-actin (4967, Cell Signaling) diluted in blocking buffer at 4°C for 16 h ...
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bioRxiv - Neuroscience 2019Quote: ... anti-phospho-CaMKII (T286, 1:1000), anti-phospho-S6K (T389, 1:500) and anti-S6K (1:500) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-CaMKII (1:500 ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then washed and stained with a primary monoclonal antibody conjugated to Alexa Fluor 647 recognizing phosphorylated JNK (Cell Signaling Technologies, Cat.#: 9257) overnight at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Total and phosphorylated glycogen synthase kinase 3 (GSK3-β) were detected using rabbit antibodies anti-GSK3-β (9315) and anti-pGSK3α/β-Ser21/9 (9331) (Cell Signaling). Chemiluminescence signals were analyzed using Image Lab software 6.0 (Bio-Rad).
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bioRxiv - Biophysics 2019Quote: ... chemically fixed cells were permeabilized in 0.1% Triton-X 100 then labeled with anti-human phosphotyrosine CD79A (Tyr182) rabbit antibody (Cell Signaling Technology Cat# 5173, RRID:AB_10694763). The primary antibody was detected using one of two a secondary goat anti rabbit polyclonal antibodies ...
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated overnight at 4°C in the primary antibodies against c-Fos (1:6400; catalog #2250S; Cell Signaling Technology, Danvers, MA), and then incubated with the secondary Alexa Fluor® 448 (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000, Cell Signaling Technology, Leiden, The Netherlands) or superoxide dismutase 2 (SOD2 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with the rabbit monoclonal anti-FosB primary antibody (dilution: 1:1000, product number: 2251S, Cell Signaling Technology, Danver, MA, USA) at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... Wing discs were incubated at 4°C for 16 hours in PBS with 0.1% Triton X-100 containing either anti-phospho-Histone H3 (Ser10) antibody (Cell Signaling Technology #9701, 1:1000) for a proliferation marker or anti-cleaved caspase-3 (Asp175 ...
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bioRxiv - Microbiology 2021Quote: ... The extent of protein knockdown was determined by immunoblotting and total loading was detected by probing the blots with an anti-GAPDH antibody (Cell Signaling; #5174, clone D16H11).
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with a 1:500 dilution of Alexa Fluor® 488-labeled rabbit anti-His tag antibody (Cell Signaling Technology, Danvers, MA) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 50μg of chromatin were immunoprecipitated overnight at 4°C with an antibody against Rev-erb-α (13418S, Cell signaling Technology, Danvers, Massachusetts, United States). BSA/yeast tRNA-blocked Protein A/G dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were saturated with the blocking solution followed by a 1hr at 37°C incubation with the primary antibodies: Bcl-xL (Cell Signaling #2764, 1/1000) and IP3R C-terminal (abcam #ab190239 ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were then washed and incubated for 1 h at room temperature with horseradish peroxidase (HRP)-conjugated secondary antibodies (sc-516102/sc-2357, Santa Cruz Biotechnology, USA; or 7077, Cell Signaling Technology, USA). Immunoreactive proteins were detected using the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (34580 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with blocking solution for 90min then with a combination of two primary antibodies: rabbit anti-Fos (1:2000; Cell Signaling Technology, RRID #2250) and chicken anti-mCherry (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then washed 3 times with TBST and the secondary goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated antibody (1:5000; cat. no. 7074, Cell Signaling Technology, The Netherlands) was added and allowed to bind at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... The following primary antibodies were used for western blot in this study: anti-Notch1 (D1E11, Cell Signaling, or mN1A, Novus, or C-20, SCBT) for Notch1 full length and cleaved forms ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis, France) and rabbit polyclonal anti-cleaved caspase 3 antibody was provided by Cell Signaling Technology (Ozyme, Saint Cyr l’ecole, France). All Alexa-conjugated secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Cancer Biology 2021Quote: ... The amplification time was 100 minutes and the primary antibodies pair to detect the interaction was rabbit anti-Aurora-A (14475, Cell Signaling, 1:100 o.n.) and mouse anti-N-Myc (NCM II 100 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed with 1× TBS for 20 min and then incubated with anti-β-actin primary antibody (1:10000 dilution; Cell Signaling Technology, 3700S) in 1× TBST with 5% milk for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...