Labshake search
Citations for Cell Signaling Technology :
8551 - 8600 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Detection of pSTAT3 was performed using a PathScan® Phospho-Stat3 (pTyr705) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7300). Cell lysates were added to pre-coated ELISA wells and incubated at 4°C overnight ...
-
bioRxiv - Immunology 2023Quote: ... Detection of pSTAT6 was performed using a PathScan® Phospho-Stat6 (pTyr641) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7275). Detection of pSTAT3 was performed using a PathScan® Phospho-Stat3 (pTyr705 ...
-
bioRxiv - Immunology 2023Quote: ... Detection of pSTAT5 was performed using a PathScan® Phospho-Stat5 (pTyr694) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7113). Detection of pSTAT6 was performed using a PathScan® Phospho-Stat6 (pTyr641 ...
-
bioRxiv - Cancer Biology 2023Quote: Samples for ChIP-seq experiments were prepared using the SimpleChIP Plus Enzymatic chromatin IP kit (Cell Signaling Technology, Cat. 9005) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Senescence-associated β-galactosidase staining was performed using a senescence β-galactosidase staining kit (Cell Signaling Technology, cat. no. 9860) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
-
bioRxiv - Pathology 2024Quote: ... was used as secondary for 30 min and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling). For IF ...
-
bioRxiv - Genetics 2019Quote: ... the membranes were incubated for 12 hours or longer at 4°C with rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 1:1000 dilution) or mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
-
bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% skim milk in TBST for 1 h at room temperature and incubated with anti-human p53 antibody (Cell Signaling Technologies, #9282, rabbit polyclonal, 1:1000) in TBST containing 5% skim milk (4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... peroxidase and haematoxylin staining were performed on deparaffinised sections that were sequentially incubated with rabbit anti-insulin antibody (Cell Signaling Technology C27C9, Danvers, MA, USA, 1:400), then with F(ab’)2 conjugates with Biotin-SP (Jackson ImmunoResearch 711-066-152 ...
-
bioRxiv - Cell Biology 2021Quote: ... Sections were processed for Nissl staining (Cresyl violet staining) or immunohistochemistry with antibodies against cleaved caspase-3 (Asp175; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:500) and c-Fos (PC05 ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated respectively with the primary antibodies (Table S1) (Tau5, Cell Signaling Technology, 46687S, 1:200; Tau46, Cell Signaling Technology, 4019S, 1:200; Phospho-Tau (Thr181), Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2022Quote: ... with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505; anti-HA, Cell Signaling Technology, #3724S; anti-MBD3, Cell Signaling Technology, #99169S ...
-
bioRxiv - Zoology 2022Quote: ... The sections were then incubated with anti-phosphorylated-p44/42 mitogen-activated protein kinase (MAPK; extracellular regulated kinase, ERK1/2) rabbit monoclonal antibody (#4370, Cell Signaling Technology, Danvers, MA, USA; 1:500), which was used in zebrafish OE36 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Primary antibody incubation was carried out overnight in OBB 1:1 in PBS with the anti phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit monoclonal antibody (#4370 Cell Signaling technology. Used at 1:2000 dilution), the monoclonal anti GFP antibody ((0.4mg/ml) ...
-
bioRxiv - Pathology 2021Quote: ... the membranes were incubated in a blocking buffer containing the appropriate diluent of primary antibodies against apoA5 (#3335, Cell Signaling Technology, USA; sc-373950, Santa Cruz, USA), tubulin (66031-1-lg ...
-
bioRxiv - Molecular Biology 2021Quote: Western blot membranes were incubated with primary antibodies against Col1 (rabbit polyclonal 1:500, Millipore Cat# AB765P, for fibroblasts and 1:1000 Cell Signaling Technology Cat# 84336, for LX2 cells), hypusinated eIF5A (FabHpu antibody ...
-
bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked for 10 minutes in 10% BSA in TBS-T and then incubated overnight at 4°C with the indicated antibodies obtained from Cell Signaling Technology (phospho-PKC Substrate Motif (#6967), phospho-p42/44 MAPK (#4370) ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated for 48 hr at 4C in 1× PBS containing the primary antibodies (Chicken anti-GFP, AbCam cat. No. #13970. 1:2000; Rabbit anti-NeuN, Cell Signaling Technology, cat. No. #24307, 1:1000). Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488 ...
-
Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Then membranes were incubated for 1 h at RT with secondary anti-mouse or anti-rabbit HRP-linked antibodies (Cell Signaling Technology #7076 or # 7074; 1:5000) diluted in Bullet Blocking One and were washed three times for 15 min in 1×TBST buffer ...
-
bioRxiv - Plant Biology 2022Quote: ... MPK phosphorylation was determined using protein extracts obtained from elicitor or control-treated leaf disks using immunoblots probed with antibodies against phosphor-p44/42 ERK (Cell Signaling Technology, Beverly, MA, USA; Ozyme S4370S) and horseradish peroxidase (HRP)-labelled secondary antibodies for luminescence detection (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
-
bioRxiv - Neuroscience 2022Quote: ... At least 3 independent controls and treated samples were probed with the following primary antibodies (in 5% milk, overnight at 4°C): YAP (1:500, Cell signaling Tec., Cat. No. 14074, RRID: AB_2650491), p YAP (1:2000 ...
-
bioRxiv - Biochemistry 2024Quote: ... for 1 hr at room temperature followed by 48 hr incubation with primary antibodies (syntenin #ab133267 from Abcam, flotillin-1 #610821 from BD Biosciences; ApoA-1 #3350 from Cell Signaling Technology and ApoB #139401 from Abcam) at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 0.1% Tween PBS supplemented with 5% (w/v) skimmed milk and subjected to immunoblotting with the following primary antibodies at the indicated dilutions: TBK1 (Cell Signaling [CS-3013], rabbit polyclonal, 1:500); GAPDH (Invitrogen [AM4300] ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were blotted on Nitrocellulose membrane (Cytiva) and revealed using following antibodies: mouse anti-AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000, rabbit anti-XBP1s (Cell Signaling Technologies, clone E9V3E, 1/1000), rabbit β-Tubulin-HRP (Abcam ...
-
bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked in 5% evaporated milk in Tris-based saline with Tween 20 (0.05% TBST) for 1 hour and followed by incubating with primary antibodies overnight at 4 °C (Anti-HA: Cell Signaling Technology, 3724S; anti-GAPDH: Thermo, MA5-15738). After three rinses with TBST ...
-
bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology, A21247 Life Tech). F-actin was stained with Alexa Fluor-conjugated Phalloidin (1:40 ...
-
bioRxiv - Microbiology 2023Quote: ... membranes were incubated in primary antibody solutions consisting of fresh blocking solution containing CCNT1 antisera (Santa Cruz Biosciences, Dallas, TX, USA, sc-398695; Cell Signaling Technologies, Danvers, MA, USA D1B6G), CDK9 antisera (Bethyl Laboratories ...
-
bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
-
bioRxiv - Developmental Biology 2020Quote: ... and incubated with annexin V and propidium iodide (PI) as per the manufacturer’s instructions (Early Apoptosis Detection Kit, Cell Signaling Technology). Cells were analyzed by flow cytometry using a BD FACSCanto cell analyzer (BD Biosciences) ...
-
bioRxiv - Physiology 2022Quote: The acetyl peptide enrichment was performed on 3 mg of digested peptides using PTMscan Acetyl-Lysine Motif kit (Cell Signaling #13416). The peptides were mixed with 100 µL of 10x IP buffer (500 mM MOPS ...
-
bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal activity staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
-
bioRxiv - Biochemistry 2020Quote: ... and measured IR phosphorylation by using the PathScan® Phospho-Insulin Receptor β (panTyr) Sandwich ELISA Kit (Cell Signaling Technology; #7082).
-
bioRxiv - Systems Biology 2019Quote: ... and monomethyl arginine peptides were immunoprecipitated by addition of 40 uL of PTMScan Symmetric Di-Methyl Arginine Motif Kit (13563, Cell Signaling), PTMScan Asymmetric Di-Methyl Arginine Motif Kit (13474 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
-
bioRxiv - Cell Biology 2021Quote: Chromatin immunoprecipitation assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit - Magnetic Beads (Cell Signaling Technology, Inc., Danvers, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... Senescence-Associated β-galactosidase activity was assessed using a SA-β-galactosidase staining kit according to the manufacturer’s instructions (Cell Signaling). Positive cells were counted manually out of more than 100 cells total.
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of the HRP/ peroxidase enzyme was performed with SignalStain® DAB Substrate Kit from Cell Signaling (cat. no. 8059; www.cellsignal.com), per the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: CUT&RUN experiments were conducted using 200,000 PS or PSP cells using the CUT&RUN assay kit (Cell Signaling Technology, 86652). Briefly ...
-
bioRxiv - Cancer Biology 2019Quote: MEFs were prepared from E13.5 embryos and senescence of MEFs was determined by using the Senescence β-Galactosidase Staining Kit (Cell Signaling) according to the manufacturer’s protocol57 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... All ChIP was performed using the SimpleChIP Enzymatic Chromatin IP kit with magnetic beads according to the manufacturer’s instructions (Cell Signaling Technologies). DNA levels were quantified by qPCR amplification using Power SYBR Green Mastermix (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2021Quote: GTP-bound GTPase pull-down assays were performed using the Active Rac1/CDC42 Detection Kit (Cell Signaling Technology, Cat. #8815, 8819). After transfection or verteporfin treatment ...
-
bioRxiv - Neuroscience 2020Quote: ... or WT N2aC plated in chamber slides using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology, Danvers, MA). As a positive control for apoptosis and necrosis ...
-
bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
-
bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.