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Citations for Cell Signaling Technology :
8301 - 8350 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... at 1:1000 and then incubated with the appropriate secondary antibody conjugated with horseradish peroxidase (1:5000, Santa Cruz, sc-2005 or 1:5000, Cell Signaling Technology, 7074S). Signal was detected using the ChemiDoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... GAPDH (1:4000, #2118), Phospho-Akt (Ser473) (1:500, #9271) and Akt antibody (1:1000, #9272) were obtained from Cell Signaling Technology (Beverly, MA). Human VEGFR3/Flt-4 antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C in the following primary antibodies: rabbit anti-cleaved NOTCH1 (Val 1744) at 1:1000 (Cell signaling technology, Cat #: 4147S), rabbit anti-NOTCH1 (D1E11 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074) or goat anti-mouse IgG (1:3,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The membrane was washed four times in 0.1% TBST buffer and incubated for 1 h at room temperature with anti-rabbit IgG conjugated to HRP-linked antibody #7074 (Cell Signaling Technology, Danvers, MA) diluted 1:5,000 with 0.05% TBST containing 5% skimmed milk ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated with the following primary antibodies for 60 min at room temperature: anti-Cas9 (1:1000 dilution; #14697S, Cell Signaling Technology, Danvers, MA), anti-MDM2 (1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG at 1:5000 dilution or anti-mouse IgG at 1:5000 dilution; both from Cell Signaling Technology, Danvers, MA), and the bound proteins were visualized using a chemiluminescence-based detection kit (ImmunoStar Zeta ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 0.5% BSA–PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (30 ng/ml) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Biophysics 2023Quote: ... and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat. 7076; anti-rabbit IgG, Cell Signaling, cat. 7074) diluted 1:5000 in 5% non-fat dried milk in TBS-T solution ...
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bioRxiv - Genetics 2023Quote: ... Cells were blocked with 5 mg/ml BSA in 1X PBS for 30 minutes and then incubated with primary antibody phospho-histone H2AX Ser139 20E3 (Cell Signaling #9718, 1:500) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, Catalog # 7074) or goat anti-mouse IgG (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074). Then the membranes were washed with TBST four times ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween 20 and 5% BSA overnight at 4 °C with anti-Runx2 antibody (RUNX2 D1H7 Rabbit mAb, Cell Signaling Technology, 84866 1:2000) and anti-β-actin antibody (Rabbit Anti-beta Actin ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Tris-buffered saline/0.1% Tween (TBST)/5% milk and probed with antibodies against E-Cadherin (Cell Signaling Technology, 3195S, 1:1000 dilution), Vimentin (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 200 μl of 1XDPBS + 1% BSA + 0.1% Tween-20 containing 4 μl of Alexa Fluor 488-conjugated phospho-histone H3 (Ser10) antibody (Cell Signaling Technologies, 1:50 dilution) for 90 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution), EAAT3 antibody (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&RUN was performed from 500,000 cells per condition in triplicates as previously described in (Skene et al, 2018) using the following antibodies: rabbit anti-HA (Cell Signaling Technology, Cat# 3724); rabbit anti-MLL1 (Epicypher ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... for 1h at RT and then immunolabeled with rabbit primary antibodies, anti-GIRK2 (1:500, Alomone labs, APC-006, RRID: AB_2040115) and anti-actin (1:2000, Cell Signaling Technology, 4970S, RRID: AB_2223172) overnight at 4°C in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilized with 0.5% Triton X-100 in blocking buffer for 15 min and incubated with primary antibodies against internal epitopes (DARPP32 (1:500, Cell Signaling Technology 2306, RRID:AB_823479) or PSD95 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibodies were used for Western blots with the indicated dilution factors: Hexokinase I (C35C4) Rabbit mAb (Cell Signaling Technology, Cat# 2024, RRID:AB_2116996) at 1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated in the following primary antibodies diluted in blocking solution: anti-E-cadherin (24E10, CST 3195, Cell Signaling, dilution 1:200), anti-ezrin (E1281 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with the following primary antibodies for 30 minutes at room temperature: anti-Vimentin (1:100 dilution; Cell Signaling Technology, cat. 5741), anti-Epcam (1:100 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylated MAPK was detected using the phospho-p44/42 MAPK antibody (for monitoring phosphorylated MAPK at Thr202/Tyr204 and marked as pTEpY in figures) (1:2,000 dilution, Cell Signaling Technology, Cat. No. 4370).
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bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The FGFR1-probed membranes were washed 5x for 5min in TBST and then probed with anti-rabbit HRP-conjugated antibody (7074S, Cell Signaling, Danvers, MA, USA) for 1h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then blocked in 2% BSA-PBS solution for 1 hr at room temperature and then incubated with a primary antibody against β-catenin (1:100; Cell signaling technology, #9562) diluted in 2% BSA-PBS ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell lysates were prepared in situ and analysed by western blotting as previously described (14) using antibodies to the following: ASNS (20843, Cell Signaling Technology; 1:1000), phospho-S6 ribosomal protein S235/236 (4858 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% BSA diluted in TBS-Tween0,1% for 1 hour and primary antibodies were incubated overnight (rabbit anti-HER2 #29D8 Cell Signaling; mouse anti-vinculin #A250291). Donkey anti-mouse IgG or goat anti-rabbit IgG secondary antibody conjugated to horse-radish peroxidase (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used for western blotting included rabbit anti-PIEZO1 (custom generated, 1:2,000) and rabbit anti-β-actin (Cell Signaling Technology, 1:3,000).
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bioRxiv - Biochemistry 2023Quote: ... The expression of MBP-FoxP3ΔN was validated by Western blot using Mouse MBP Tag antibody(8G1) (Cell Signaling Technology, Cat#2396, 1:2000 dilution).
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bioRxiv - Immunology 2023Quote: ... and incubated overnight at 4°C with mild agitation in 5% milk in TBST plus indicated antibody: cytochrome c (1:750, rabbit, Cell Signaling Technology, Cat. 11940), GAPDH (1:10000 ...
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bioRxiv - Plant Biology 2024Quote: ... and MAPK activation was assessed by immunoblot analysis using primary anti-Phospho-p44/42 MAPK antibody (Erk1/I 2; Thr-202/Tyr-204) (Cell Signaling, Danvers, MA, USA) and secondary goat anti-rabbit-HRP antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissues were then incubated overnight at 4°C with anti-DeltaFosB primary antibody (DeltaFOSB; rabbit; Cell Signaling Technology, ref. 22509; 1:1000 dilution) or anti-Fos antibody (c-Fos ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed three times for 10 min each with 1x TBST and incubated with a horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology; Cat #7074S) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies, Cat. # 13174) and an anti- rabbit HRP secondary antibody (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Samples probed with rabbit primary antibodies were subsequently probed with horseradish peroxidase (HRP)-linked anti-rabbit IgG (1:2500 dilution; Cell Signaling Technologies; item # 7074) secondary antibodies ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cell pellets were lysed and lysates were prepared and analyzed as described above in “Lysate preparation and immunoblotting,” using anti-α-tubulin antibodies (#2144, Cell Signaling Technology, USA) at a 1:10,000 dilution in TBS-T ...